ID LHX4_MOUSE Reviewed; 390 AA.
AC P53776; O08916; Q810K7; Q9R280;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 4.
DT 27-MAR-2024, entry version 175.
DE RecName: Full=LIM/homeobox protein Lhx4;
DE Short=LIM homeobox protein 4;
GN Name=Lhx4; Synonyms=Gsh-4, Gsh4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-390.
RA Sakai T., Kawaguchi A., Nagashima M.;
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 30-219.
RC STRAIN=FVB/N; TISSUE=Embryo;
RX PubMed=9286712; DOI=10.1006/geno.1997.4852;
RA Yamashita T., Moriyama K., Sheng H.Z., Westphal H.;
RT "Lhx4, a LIM homeobox gene.";
RL Genomics 44:144-146(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 161-390.
RX PubMed=7913017; DOI=10.1002/j.1460-2075.1994.tb06582.x;
RA Li H., Witte D.P., Branford W.W., Aronow B.J., Weinstein M., Kaur S.,
RA Wert S., Singh G., Schreiner C.M., Whitsett J.A., Scott W.J. Jr.,
RA Potter S.S.;
RT "Gsh-4 encodes a LIM-type homeodomain, is expressed in the developing
RT central nervous system and is required for early postnatal survival.";
RL EMBO J. 13:2876-2885(1994).
CC -!- FUNCTION: May play a critical role in the development of respiratory
CC control mechanisms and in the normal growth and maturation of the lung.
CC Binds preferentially to methylated DNA (By similarity).
CC {ECO:0000250|UniProtKB:Q969G2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- TISSUE SPECIFICITY: Transient expression in ventrolateral regions of
CC the developing neural tube and hindbrain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD30125.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC049834; AAH49834.1; -; mRNA.
DR EMBL; AF135415; AAD30125.1; ALT_INIT; mRNA.
DR EMBL; U89343; AAC53336.1; -; mRNA.
DR EMBL; S71659; AAB31260.1; -; mRNA.
DR CCDS; CCDS48399.1; -.
DR PIR; S46332; S46332.
DR RefSeq; NP_034842.2; NM_010712.2.
DR PDB; 3MMK; X-ray; 2.16 A; A/B=24-149.
DR PDB; 6CME; X-ray; 1.92 A; A/B=24-149.
DR PDBsum; 3MMK; -.
DR PDBsum; 6CME; -.
DR AlphaFoldDB; P53776; -.
DR SASBDB; P53776; -.
DR SMR; P53776; -.
DR BioGRID; 201157; 3.
DR IntAct; P53776; 3.
DR STRING; 10090.ENSMUSP00000027740; -.
DR iPTMnet; P53776; -.
DR PhosphoSitePlus; P53776; -.
DR PaxDb; 10090-ENSMUSP00000027740; -.
DR Antibodypedia; 20587; 406 antibodies from 28 providers.
DR DNASU; 16872; -.
DR Ensembl; ENSMUST00000027740.14; ENSMUSP00000027740.8; ENSMUSG00000026468.15.
DR GeneID; 16872; -.
DR KEGG; mmu:16872; -.
DR UCSC; uc007dbn.2; mouse.
DR AGR; MGI:101776; -.
DR CTD; 89884; -.
DR MGI; MGI:101776; Lhx4.
DR VEuPathDB; HostDB:ENSMUSG00000026468; -.
DR eggNOG; KOG4577; Eukaryota.
DR GeneTree; ENSGT00940000157906; -.
DR HOGENOM; CLU_027802_5_0_1; -.
DR InParanoid; P53776; -.
DR OMA; GLDYTMD; -.
DR OrthoDB; 932158at2759; -.
DR PhylomeDB; P53776; -.
DR TreeFam; TF315442; -.
DR BioGRID-ORCS; 16872; 2 hits in 78 CRISPR screens.
DR PRO; PR:P53776; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P53776; Protein.
DR Bgee; ENSMUSG00000026468; Expressed in pineal body and 75 other cell types or tissues.
DR ExpressionAtlas; P53776; baseline and differential.
DR Genevisible; P53776; MM.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI.
DR GO; GO:0006915; P:apoptotic process; IMP:MGI.
DR GO; GO:0021526; P:medial motor column neuron differentiation; IGI:MGI.
DR GO; GO:0008045; P:motor neuron axon guidance; IGI:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0001890; P:placenta development; IGI:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd09468; LIM1_Lhx4; 1.
DR CDD; cd09376; LIM2_Lhx3_Lhx4; 1.
DR Gene3D; 2.10.110.10; Cysteine Rich Protein; 2.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR049594; Lhx3/4-like_LIM2.
DR InterPro; IPR047956; LHX4_LIM1.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24208; LIM/HOMEOBOX PROTEIN LHX; 1.
DR PANTHER; PTHR24208:SF116; LIM_HOMEOBOX PROTEIN LHX4; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 2.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..390
FT /note="LIM/homeobox protein Lhx4"
FT /id="PRO_0000075788"
FT DOMAIN 28..87
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 88..150
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 157..216
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 161..181
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:Q969G2"
FT REGION 199..211
FT /note="Interaction with 5-mCpG DNA"
FT /evidence="ECO:0000250|UniProtKB:Q969G2"
FT REGION 231..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 355..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 234..253
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 377
FT /note="S -> T (in Ref. 1; AAH49834)"
FT /evidence="ECO:0000305"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 39..45
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 48..50
FT /evidence="ECO:0007829|PDB:6CME"
FT TURN 52..54
FT /evidence="ECO:0007829|PDB:6CME"
FT TURN 58..60
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 65..71
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:6CME"
FT HELIX 78..84
FT /evidence="ECO:0007829|PDB:6CME"
FT TURN 90..92
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 102..105
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:6CME"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:6CME"
FT TURN 118..120
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:6CME"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:6CME"
FT HELIX 141..147
FT /evidence="ECO:0007829|PDB:6CME"
SQ SEQUENCE 390 AA; 43078 MW; FF70967D30C724C4 CRC64;
MMQSAAVPAE GAVKGLPEML GVPMQQIPQC AGCNQHILDK FILKVLDRHW HSSCLKCADC
QMQLADRCFS RAGSVYCKED FFKRFGTKCT ACQQGIPPTQ VVRKAQDFVY HLHCFACIIC
NRQLATGDEF YLMEDGRLVC KEDYETAKQN DDSEAGAKRP RTTITAKQLE TLKNAYKNSP
KPARHVREQL SSETGLDMRV VQVWFQNRRA KEKRLKKDAG RHRWGQFYKS VKRSRGGSKQ
EKESSAEDCG VSDSELSFRE DQILSELGHT NRIYGNVGDV TGGQLMNGSF SMDGTGQSYQ
DLRDGSPYGI PQSPSSISSL PSHAPLLNGL DYTVDSNLGI IAHAGQGVSQ TLRAMAGGPT
SDLSTGSSVG YPDFPTSPAS WLDEMDHPPF
//
