ID ALDH3_YEAST Reviewed; 506 AA.
AC P54114; D6VZZ1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 27-MAR-2024, entry version 176.
DE RecName: Full=Aldehyde dehydrogenase [NAD(P)+] 2 {ECO:0000305};
DE EC=1.2.1.3 {ECO:0000269|PubMed:10407263, ECO:0000269|PubMed:12586697};
GN Name=ALD3; Synonyms=ALD4; OrderedLocusNames=YMR169C; ORFNames=YM8520.18C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND INDUCTION.
RX PubMed=10407263;
RX DOI=10.1002/(sici)1097-0061(199907)15:10a<829::aid-yea423>3.0.co;2-9;
RA Navarro-Avino J.P., Prasad R., Miralles V.J., Benito R.M., Serrano R.;
RT "A proposal for nomenclature of aldehyde dehydrogenases in Saccharomyces
RT cerevisiae and characterization of the stress-inducible ALD2 and ALD3
RT genes.";
RL Yeast 15:829-842(1999).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=12586697; DOI=10.1093/genetics/163.1.69;
RA White W.H., Skatrud P.L., Xue Z., Toyn J.H.;
RT "Specialization of function among aldehyde dehydrogenases: the ALD2 and
RT ALD3 genes are required for beta-alanine biosynthesis in Saccharomyces
RT cerevisiae.";
RL Genetics 163:69-77(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Cytoplasmic aldehyde dehydrogenase involved in ethanol
CC oxidation. Involved in pantothenic acid production through the
CC conversion of 3-aminopropanal to beta-alanine, an intermediate in
CC pantothenic acid (vitamin B5) and coenzyme A (CoA) biosynthesis.
CC {ECO:0000269|PubMed:10407263, ECO:0000269|PubMed:12586697}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH;
CC Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.2.1.3; Evidence={ECO:0000269|PubMed:10407263,
CC ECO:0000269|PubMed:12586697};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-aminopropanal + H2O + NAD(+) = beta-alanine + 2 H(+) + NADH;
CC Xref=Rhea:RHEA:30695, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:57966,
CC ChEBI:CHEBI:58374; Evidence={ECO:0000269|PubMed:10407263,
CC ECO:0000269|PubMed:12586697};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30696;
CC Evidence={ECO:0000305|PubMed:12586697};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Expression is under the control of MSN2 and MSN4, and is
CC induced during diauxic shift and osmotic stress.
CC {ECO:0000269|PubMed:10407263}.
CC -!- MISCELLANEOUS: Present with 172 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; Z49705; CAA89805.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10065.1; -; Genomic_DNA.
DR PIR; S54527; S54527.
DR RefSeq; NP_013892.1; NM_001182673.1.
DR AlphaFoldDB; P54114; -.
DR SMR; P54114; -.
DR BioGRID; 35347; 47.
DR MINT; P54114; -.
DR STRING; 4932.YMR169C; -.
DR iPTMnet; P54114; -.
DR MaxQB; P54114; -.
DR PaxDb; 4932-YMR169C; -.
DR PeptideAtlas; P54114; -.
DR EnsemblFungi; YMR169C_mRNA; YMR169C; YMR169C.
DR GeneID; 855205; -.
DR KEGG; sce:YMR169C; -.
DR AGR; SGD:S000004779; -.
DR SGD; S000004779; ALD3.
DR VEuPathDB; FungiDB:YMR169C; -.
DR eggNOG; KOG2450; Eukaryota.
DR GeneTree; ENSGT00940000176434; -.
DR HOGENOM; CLU_005391_0_1_1; -.
DR InParanoid; P54114; -.
DR OMA; GTYAINW; -.
DR OrthoDB; 216092at2759; -.
DR BioCyc; MetaCyc:YMR169C-MONOMER; -.
DR BioCyc; YEAST:YMR169C-MONOMER; -.
DR BioGRID-ORCS; 855205; 0 hits in 10 CRISPR screens.
DR PRO; PR:P54114; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P54114; Protein.
DR GO; GO:0005737; C:cytoplasm; ISS:SGD.
DR GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IDA:SGD.
DR GO; GO:0043878; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0019483; P:beta-alanine biosynthetic process; IMP:SGD.
DR GO; GO:0015940; P:pantothenate biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006598; P:polyamine catabolic process; IMP:SGD.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR016160; Ald_DH_CS_CYS.
DR InterPro; IPR029510; Ald_DH_CS_GLU.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR PANTHER; PTHR43720; 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE; 1.
DR PANTHER; PTHR43720:SF2; 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE; 1.
DR Pfam; PF00171; Aldedh; 1.
DR SUPFAM; SSF53720; ALDH-like; 1.
DR PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; NAD; Oxidoreductase; Pantothenate biosynthesis;
KW Reference proteome.
FT CHAIN 1..506
FT /note="Aldehyde dehydrogenase [NAD(P)+] 2"
FT /id="PRO_0000056440"
FT ACT_SITE 268
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10007,
FT ECO:0000255|PROSITE-ProRule:PRU10008"
FT ACT_SITE 302
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10007,
FT ECO:0000255|PROSITE-ProRule:PRU10008"
FT SITE 169
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
SQ SEQUENCE 506 AA; 55385 MW; 11687E520B7E97DD CRC64;
MPTLYTDIEI PQLKISLKQP LGLFINNEFC PSSDGKTIET VNPATGEPIT SFQAANEKDV
DKAVKAARAA FDNVWSKTSS EQRGIYLSNL LKLIEEEQDT LAALETLDAG KPFHSNAKQD
LAQIIELTRY YAGAVDKFNM GETIPLTFNK FAYTLKVPFG VVAQIVPWNY PLAMACRKMQ
GALAAGNTVI IKPAENTSLS LLYFATLIKK AGFPPGVVNV IPGYGSVVGK ALGTHMDIDK
ISFTGSTKVG GSVLEASGQS NLKDITLECG GKSPALVFED ADLDKAIEWV ANGIFFNSGQ
ICTANSRVYV QSSIYDKFVE KFKETAKKEW DVAGKFDPFD EKCIVGPVIS STQYDRIKSY
IERGKKEEKL DMFQTSEFPI GGAKGYFIPP TIFTDVPETS KLLRDEIFGP VVVVSKFTNY
DDALKLANDT CYGLASAVFT KDVKKAHMFA RDIKAGTVWI NQTNQEEAKV PFGGFKMSGI
GRESGDTGVD NYLQIKSVHV DLSLDK
//
