ID PI3K3_DICDI Reviewed; 1697 AA.
AC P54675; Q553Z1; Q869X9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 01-MAY-2013, entry version 90.
DE RecName: Full=Phosphatidylinositol 3-kinase 3;
DE Short=PI3-kinase;
DE Short=PI3K;
DE Short=PtdIns-3-kinase;
DE EC=2.7.1.137;
GN Name=pikC; Synonyms=pik3; ORFNames=DDB_G0275011;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A.,
RA Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G.,
RA Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A.,
RA Platzer M., Rosenthal A., Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 113-1697.
RC STRAIN=AX3;
RX PubMed=7565716;
RA Zhou K., Takegawa K., Emr S.D., Firtel R.A.;
RT "A phosphatidylinositol (PI) kinase gene family in Dictyostelium
RT discoideum: biological roles of putative mammalian p110 and yeast
RT Vps34p PI 3-kinase homologs during growth and development.";
RL Mol. Cell. Biol. 15:5645-5656(1995).
CC -!- CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol = ADP +
CC 1-phosphatidyl-1D-myo-inositol 3-phosphate.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC -!- SIMILARITY: Contains 1 C2 PI3K-type domain.
CC -!- SIMILARITY: Contains 1 PI3K-RBD domain.
CC -!- SIMILARITY: Contains 1 PI3K/PI4K domain.
CC -!- SIMILARITY: Contains 1 PIK helical domain.
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DR EMBL; AAFI02000013; EAL69786.1; -; Genomic_DNA.
DR EMBL; U23478; AAA85723.1; -; mRNA.
DR PIR; T18274; T18274.
DR RefSeq; XP_643820.1; XM_638728.1.
DR ProteinModelPortal; P54675; -.
DR STRING; 44689.DDB_0185203; -.
DR EnsemblProtists; DDB0185203; DDB0185203; DDB_G0275011.
DR GeneID; 8619867; -.
DR KEGG; ddi:DDB_G0275011; -.
DR dictyBase; DDB_G0275011; pikC.
DR eggNOG; COG5032; -.
DR KO; K00922; -.
DR OMA; VEIREYA; -.
DR ProtClustDB; CLSZ2846827; -.
DR BRENDA; 2.7.1.137; 1939.
DR GO; GO:0005942; C:phosphatidylinositol 3-kinase complex; IBA:RefGenome.
DR GO; GO:0005886; C:plasma membrane; IBA:RefGenome.
DR GO; GO:0016303; F:1-phosphatidylinositol-3-kinase activity; TAS:dictyBase.
DR GO; GO:0035005; F:1-phosphatidylinositol-4-phosphate 3-kinase activity; IBA:RefGenome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006928; P:cellular component movement; IGI:dictyBase.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:InterPro.
DR GO; GO:0009617; P:response to bacterium; IMP:dictyBase.
DR Gene3D; 1.10.1070.11; -; 1.
DR Gene3D; 1.25.40.70; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR008973; C2_Ca/lipid-bd_dom_CaLB.
DR InterPro; IPR011009; Kinase-like_dom.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR002420; PI3K_C2_dom.
DR InterPro; IPR000341; PI3K_Ras-bd_dom.
DR InterPro; IPR015433; PI_Kinase.
DR InterPro; IPR001263; PInositide-3_kin_accessory_dom.
DR PANTHER; PTHR10048; PTHR10048; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF00792; PI3K_C2; 1.
DR Pfam; PF00794; PI3K_rbd; 1.
DR Pfam; PF00613; PI3Ka; 1.
DR SMART; SM00142; PI3K_C2; 1.
DR SMART; SM00144; PI3K_rbd; 1.
DR SMART; SM00145; PI3Ka; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM-type_fold; 1.
DR SUPFAM; SSF49562; C2_CaLB; 1.
DR SUPFAM; SSF56112; Kinase_like; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
DR PROSITE; PS51547; PI3K_C2; 1.
DR PROSITE; PS51546; PI3K_RBD; 1.
DR PROSITE; PS51545; PIK_HELICAL; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Complete proteome; Kinase; Nucleotide-binding;
KW Reference proteome; Repeat; Transferase.
FT CHAIN 1 1697 Phosphatidylinositol 3-kinase 3.
FT /FTId=PRO_0000088824.
FT DOMAIN 737 823 PI3K-RBD.
FT DOMAIN 888 1036 C2 PI3K-type.
FT DOMAIN 1060 1238 PIK helical.
FT DOMAIN 1333 1596 PI3K/PI4K.
FT REPEAT 1622 1626 1.
FT REPEAT 1627 1631 2.
FT REPEAT 1632 1636 3.
FT REPEAT 1642 1646 4.
FT REPEAT 1647 1651 5.
FT REGION 1622 1651 5 X 5 AA approximate repeats.
FT REGION 1659 1672 7 X 2 AA tandem repeats of K-E.
FT COMPBIAS 170 196 Poly-Asn.
FT COMPBIAS 210 222 Poly-Asn.
FT COMPBIAS 312 338 Poly-Asn.
FT COMPBIAS 351 366 Poly-Asn.
FT COMPBIAS 457 490 Poly-Asn.
FT COMPBIAS 495 502 Poly-Asn.
FT COMPBIAS 596 600 Poly-Ser.
FT COMPBIAS 832 849 Poly-Gln.
FT COMPBIAS 1681 1687 Poly-Asn.
FT CONFLICT 1159 1159 A -> T (in Ref. 3; AAA85723).
FT CONFLICT 1175 1175 M -> L (in Ref. 3; AAA85723).
SQ SEQUENCE 1697 AA; 192942 MW; C5442DC2C3202E32 CRC64;
MRQIVTGVIH QTTQSQQIPN VINSNQIQFS NEPMVVGSIE DFDIDSEVPP LAINLQRSIN
NNNNNNNNNN NNNNNNNNNN NNNNNNNTQP CTTVFLDRDS CVNVKATIDL LKEQLEFTIK
DLIDFKENYD KLESTEQFKQ WSNLIKNIKE NSLNNSNIYL TIPTTQNLIN NNNNNNNNNN
NNNNNNNNNN NNNNNNVIIP SASTENKEEN DNNNSNNNNN INLSPDSSIT KDINITENKI
TEIKTTETKE TSTGTSPLEK SPSKGFIISP KKPEEENEIE GETINNIAIT NYTQGPSMLT
LMKKKLENIK KNNNNNNNNG NGNNNSNNNN SNSNNNNNGI SPSSSPPSHL NGNNNNNNSN
NTNSNNTTNA TTNSVGFSIT MTNSNSLSVS KRMNKFKSWT SSKPTSSSIG FASSPQNNGK
PLNISGSSRF FTSRQDSKID LLKSPSSSPP TQSDIFNENN NNNNNNNNNN NNNNNNNNNN
NNNNNNNNNN EELINNNNNN NNDENYKIEE TEESLKELLE KEKLENEERE KILKERNEID
NLKKKNHLSK GYFMRACNAS NDDGLEEEDI PLQDEHWETN VIVLLPCRHH VKVPGSSSSS
IDSIRQLAWA SGKMQGHLNL EKDEKFFTLR WCNKDVVFDQ DTPLGHLIQY NLNYNNPTQK
PTNIKLELVL EDELCKERLV DLQSLEINNG RPSIWKSHID DVLSFNRKLR ELAMLAKPQS
NVPAARLTPY PPPKTIPEFF VIRVHLFKNQ TKSLRCANNH TAFSLMTILS EKLKNTTPFD
PTQYRFLITG INQYVDPNVP LLSVEYIVEK IKRKGEIDLT MVELLSLGLI IQQQQQQQQQ
QQQQQQQQQI ENIDDENILK LNNGILNVLS KIEKPIREKD NCISSLTVTE NLQVRLLHAH
EIFASKASEI IGTDSPSIQL FIEAAVYFGG ELLATQSSKL VSFQDTVVWN EWVNIPLAVS
NIPNGARMCL GLNARYRGDI FNIGWVGHRL FDSKGILNTF APFSLLLWPG KINPIGTCVD
NLESKDQAII IAFEFKDYVV PKTIHYEDDL IELISKDENG NELPVVTMEE MDRVEQIILQ
DPLYSLNKEE RLLIWKSRYF CHTKPQALSK LLQSVEWTNY KQVGEAFQLL KIWPTLSAVD
ALELLDPKFA DCVEIREYAV KCLDQMSDYE LEIYMLQLVQ AIKHDVFHNS VLSLFLIGRV
WQNMQVLGHP FFWHLRADID NQEVCERFRV LSSGFLRYAP TQLMESFKRE ITTLRILENL
AKRVKEVPYE KRKQYVENNL REEQSFPTEL FVPFDPSIRI LNIIPEKCKS MDSAKVPLWV
TFKNADPFAP PLQMIAKTGD DLRQDILTLQ LLRLMDHMWK SQDLDLHMTI YRCIATGMGT
GLIEVVPNSE TAARIQAGAG GVSGAFKQTP IANWLKNHNQ TENSYQKAVS KFTLSCAGYC
VATYVLGIGD RHNDNIMVDI HGHLFHIDFG HFLGNFKTFA GFQREKAPFV LTPDFVYVIG
GKDSPNFAFF VDICCKAFNI IRSNAHVFIN MFELMLSTGI PELRSENDIV YLRDKFRLDL
TDAEASEYFK KLIHESIGTL TTTINFAIHI MAHRKNLVSG NSAPKIGSAS SLNLNKNKPS
SQSKLDLSRS DLSRSDSSRS DSSRLDLSRS DKKNNKDNKE KEKEKEKEKE KENNDNNDKD
NNNNSNNDTE KENSIDK
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