GenomeNet

Database: UniProt
Entry: P60438
LinkDB: P60438
Original site: P60438 
ID   RL3_ECOLI               Reviewed;         209 AA.
AC   P60438; P02386; Q2M6Y5;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-SEP-2014, entry version 113.
DE   RecName: Full=50S ribosomal protein L3;
GN   Name=rplC; OrderedLocusNames=b3320, JW3282;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   PROTEIN SEQUENCE, AND METHYLATION AT GLN-150.
RC   STRAIN=MRE-600;
RX   PubMed=365579; DOI=10.1016/0014-5793(78)80423-2;
RA   Muranova T.A., Muranov A.V., Markova L.F., Ovchinnikov Y.A.;
RT   "The primary structure of ribosomal protein L3 from Escherichia coli
RT   70 S ribosomes.";
RL   FEBS Lett. 96:301-305(1978).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3892488; DOI=10.1093/nar/13.12.4521;
RA   Zurawski G., Zurawski S.M.;
RT   "Structure of the Escherichia coli S10 ribosomal protein operon.";
RL   Nucleic Acids Res. 13:4521-4526(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
RX   PubMed=7037196; DOI=10.1016/0092-8674(81)90303-2;
RA   Olins P.O., Nomura M.;
RT   "Regulation of the S10 ribosomal protein operon in E. coli: nucleotide
RT   sequence at the start of the operon.";
RL   Cell 26:205-211(1981).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-12.
RC   STRAIN=K12 / EMG2;
RX   PubMed=9298646; DOI=10.1002/elps.1150180807;
RA   Link A.J., Robison K., Church G.M.;
RT   "Comparing the predicted and observed properties of proteins encoded
RT   in the genome of Escherichia coli K-12.";
RL   Electrophoresis 18:1259-1313(1997).
RN   [7]
RP   IDENTIFICATION AS AN ASSEMBLY INITIATOR PROTEIN.
RX   PubMed=6760192; DOI=10.1073/pnas.79.23.7238;
RA   Nowotny V., Nierhaus K.H.;
RT   "Initiator proteins for the assembly of the 50S subunit from
RT   Escherichia coli ribosomes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7238-7242(1982).
RN   [8]
RP   ASSEMBLY MAP OF THE 50S SUBUNIT.
RC   STRAIN=K12;
RX   PubMed=3298242;
RA   Herold M., Nierhaus K.H.;
RT   "Incorporation of six additional proteins to complete the assembly map
RT   of the 50 S subunit from Escherichia coli ribosomes.";
RL   J. Biol. Chem. 262:8826-8833(1987).
RN   [9]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R.,
RA   Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
RN   [10]
RP   MASS SPECTROMETRY.
RC   STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX   PubMed=10094780; DOI=10.1006/abio.1998.3077;
RA   Arnold R.J., Reilly J.P.;
RT   "Observation of Escherichia coli ribosomal proteins and their
RT   posttranslational modifications by mass spectrometry.";
RL   Anal. Biochem. 269:105-112(1999).
RN   [11]
RP   METHYLATION BY PRMB.
RC   STRAIN=K12;
RX   PubMed=11847124; DOI=10.1093/emboj/21.4.769;
RA   Heurgue-Hamard V., Champ S., Engstroem A., Ehrenberg M.,
RA   Buckingham R.H.;
RT   "The hemK gene in Escherichia coli encodes the N(5)-glutamine
RT   methyltransferase that modifies peptide release factors.";
RL   EMBO J. 21:769-778(2002).
RN   [12]
RP   SUCCINYLATION AT LYS-38.
RC   STRAIN=K12;
RX   PubMed=21151122; DOI=10.1038/nchembio.495;
RA   Zhang Z., Tan M., Xie Z., Dai L., Chen Y., Zhao Y.;
RT   "Identification of lysine succinylation as a new post-translational
RT   modification.";
RL   Nat. Chem. Biol. 7:58-63(2011).
RN   [13]
RP   STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
RC   STRAIN=MRE-600;
RX   PubMed=12809609; DOI=10.1016/S0092-8674(03)00427-6;
RA   Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M.,
RA   Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S.,
RA   Frank J.;
RT   "Study of the structural dynamics of the E. coli 70S ribosome using
RT   real-space refinement.";
RL   Cell 113:789-801(2003).
RN   [14]
RP   X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME
RP   STRUCTURES.
RC   STRAIN=MRE-600;
RX   PubMed=16272117; DOI=10.1126/science.1117230;
RA   Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W.,
RA   Vila-Sanjurjo A., Holton J.M., Cate J.H.D.;
RT   "Structures of the bacterial ribosome at 3.5 A resolution.";
RL   Science 310:827-834(2005).
CC   -!- FUNCTION: One of two assembly initiator proteins, it binds
CC       directly near the 3'-end of the 23S rRNA, where it nucleates
CC       assembly of the 50S subunit.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit. Forms a cluster with
CC       proteins L14 and L19.
CC   -!- PTM: Methylated by PrmB (By similarity).
CC   -!- MASS SPECTROMETRY: Mass=22257.2; Method=MALDI; Range=1-209;
CC       Source=PubMed:10094780;
CC   -!- SIMILARITY: Belongs to the ribosomal protein L3P family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X02613; CAA26460.1; -; Genomic_DNA.
DR   EMBL; U18997; AAA58117.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76345.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77971.1; -; Genomic_DNA.
DR   EMBL; V00344; CAA23634.1; -; Genomic_DNA.
DR   PIR; A02757; R5EC3.
DR   RefSeq; NP_417779.1; NC_000913.3.
DR   RefSeq; YP_492112.1; NC_007779.1.
DR   PDB; 1ML5; EM; 14.00 A; e=2-209.
DR   PDB; 1P85; EM; 12.30 A; B=1-209.
DR   PDB; 1P86; EM; 11.50 A; B=1-209.
DR   PDB; 1VS6; X-ray; 3.46 A; D=1-209.
DR   PDB; 1VS8; X-ray; 3.46 A; D=1-209.
DR   PDB; 1VT2; X-ray; 3.30 A; D=1-209.
DR   PDB; 2AW4; X-ray; 3.46 A; D=1-209.
DR   PDB; 2AWB; X-ray; 3.46 A; D=1-209.
DR   PDB; 2GYA; EM; 15.00 A; B=1-209.
DR   PDB; 2GYC; EM; 15.00 A; B=1-209.
DR   PDB; 2I2T; X-ray; 3.22 A; D=1-209.
DR   PDB; 2I2V; X-ray; 3.22 A; D=1-209.
DR   PDB; 2J28; EM; 8.00 A; D=1-209.
DR   PDB; 2QAM; X-ray; 3.21 A; D=1-209.
DR   PDB; 2QAO; X-ray; 3.21 A; D=1-209.
DR   PDB; 2QBA; X-ray; 3.54 A; D=1-209.
DR   PDB; 2QBC; X-ray; 3.54 A; D=1-209.
DR   PDB; 2QBE; X-ray; 3.30 A; D=1-209.
DR   PDB; 2QBG; X-ray; 3.30 A; D=1-209.
DR   PDB; 2QBI; X-ray; 4.00 A; D=1-209.
DR   PDB; 2QBK; X-ray; 4.00 A; D=1-209.
DR   PDB; 2QOV; X-ray; 3.93 A; D=1-209.
DR   PDB; 2QOX; X-ray; 3.93 A; D=1-209.
DR   PDB; 2QOZ; X-ray; 3.50 A; D=1-209.
DR   PDB; 2QP1; X-ray; 3.50 A; D=1-209.
DR   PDB; 2RDO; EM; 9.10 A; D=1-209.
DR   PDB; 2VHM; X-ray; 3.74 A; D=1-209.
DR   PDB; 2VHN; X-ray; 3.74 A; D=1-209.
DR   PDB; 2WWQ; EM; 5.80 A; D=1-209.
DR   PDB; 2Z4L; X-ray; 4.45 A; D=1-209.
DR   PDB; 2Z4N; X-ray; 4.45 A; D=1-209.
DR   PDB; 3BBX; EM; 10.00 A; D=1-209.
DR   PDB; 3DF2; X-ray; 3.50 A; D=1-209.
DR   PDB; 3DF4; X-ray; 3.50 A; D=1-209.
DR   PDB; 3E1B; EM; -; Y=1-209.
DR   PDB; 3E1D; EM; -; Y=1-209.
DR   PDB; 3FIK; EM; 6.70 A; D=1-209.
DR   PDB; 3I1N; X-ray; 3.19 A; D=1-209.
DR   PDB; 3I1P; X-ray; 3.19 A; D=1-209.
DR   PDB; 3I1R; X-ray; 3.81 A; D=1-209.
DR   PDB; 3I1T; X-ray; 3.81 A; D=1-209.
DR   PDB; 3I20; X-ray; 3.71 A; D=1-209.
DR   PDB; 3I22; X-ray; 3.71 A; D=1-209.
DR   PDB; 3IZT; EM; -; E=1-209.
DR   PDB; 3IZU; EM; -; E=1-209.
DR   PDB; 3J01; EM; -; D=1-209.
DR   PDB; 3J0T; EM; 12.10 A; E=1-209.
DR   PDB; 3J0W; EM; 14.70 A; E=1-209.
DR   PDB; 3J0Y; EM; 13.50 A; E=1-209.
DR   PDB; 3J11; EM; 13.10 A; E=1-209.
DR   PDB; 3J12; EM; 11.50 A; E=1-209.
DR   PDB; 3J14; EM; 11.50 A; E=1-209.
DR   PDB; 3J19; EM; 8.30 A; D=1-209.
DR   PDB; 3J37; EM; 9.80 A; E=1-209.
DR   PDB; 3J4X; EM; 12.00 A; D=1-209.
DR   PDB; 3J50; EM; 20.00 A; D=1-209.
DR   PDB; 3J51; EM; 17.00 A; D=1-209.
DR   PDB; 3J52; EM; 12.00 A; D=1-209.
DR   PDB; 3J54; EM; 13.00 A; D=1-209.
DR   PDB; 3J56; EM; 15.00 A; D=1-209.
DR   PDB; 3J58; EM; 17.00 A; D=1-209.
DR   PDB; 3J5A; EM; 12.00 A; D=1-209.
DR   PDB; 3J5C; EM; 17.00 A; D=1-209.
DR   PDB; 3J5E; EM; 17.00 A; D=1-209.
DR   PDB; 3J5G; EM; 20.00 A; D=1-209.
DR   PDB; 3J5I; EM; 15.00 A; D=1-209.
DR   PDB; 3J5K; EM; 9.00 A; D=1-209.
DR   PDB; 3J5L; EM; 6.60 A; D=1-209.
DR   PDB; 3J5O; EM; 6.80 A; D=1-209.
DR   PDB; 3J5U; EM; 7.60 A; E=1-209.
DR   PDB; 3J5W; EM; 7.60 A; E=1-209.
DR   PDB; 3KCR; EM; -; D=1-209.
DR   PDB; 3OAS; X-ray; 3.25 A; D=1-209.
DR   PDB; 3OAT; X-ray; 3.25 A; D=1-209.
DR   PDB; 3OFC; X-ray; 3.19 A; D=1-209.
DR   PDB; 3OFD; X-ray; 3.19 A; D=1-209.
DR   PDB; 3OFQ; X-ray; 3.10 A; D=1-209.
DR   PDB; 3OFR; X-ray; 3.10 A; D=1-209.
DR   PDB; 3OFZ; X-ray; 3.29 A; D=1-209.
DR   PDB; 3OG0; X-ray; 3.29 A; D=1-209.
DR   PDB; 3ORB; X-ray; 3.30 A; D=1-209.
DR   PDB; 3R8S; X-ray; 3.00 A; D=1-209.
DR   PDB; 3R8T; X-ray; 3.00 A; D=1-209.
DR   PDB; 3SGF; X-ray; 3.20 A; D=1-209.
DR   PDB; 3UOS; X-ray; 3.70 A; D=1-209.
DR   PDB; 4CSU; EM; 5.50 A; D=1-209.
DR   PDB; 4GAR; X-ray; 3.30 A; D=1-209.
DR   PDB; 4GAU; X-ray; 3.30 A; D=1-209.
DR   PDB; 4KIX; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KIZ; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KJ1; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KJ3; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KJ5; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KJ7; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KJ9; X-ray; 2.90 A; D=1-209.
DR   PDB; 4KJB; X-ray; 2.90 A; D=1-209.
DR   PDBsum; 1ML5; -.
DR   PDBsum; 1P85; -.
DR   PDBsum; 1P86; -.
DR   PDBsum; 1VS6; -.
DR   PDBsum; 1VS8; -.
DR   PDBsum; 1VT2; -.
DR   PDBsum; 2AW4; -.
DR   PDBsum; 2AWB; -.
DR   PDBsum; 2GYA; -.
DR   PDBsum; 2GYC; -.
DR   PDBsum; 2I2T; -.
DR   PDBsum; 2I2V; -.
DR   PDBsum; 2J28; -.
DR   PDBsum; 2QAM; -.
DR   PDBsum; 2QAO; -.
DR   PDBsum; 2QBA; -.
DR   PDBsum; 2QBC; -.
DR   PDBsum; 2QBE; -.
DR   PDBsum; 2QBG; -.
DR   PDBsum; 2QBI; -.
DR   PDBsum; 2QBK; -.
DR   PDBsum; 2QOV; -.
DR   PDBsum; 2QOX; -.
DR   PDBsum; 2QOZ; -.
DR   PDBsum; 2QP1; -.
DR   PDBsum; 2RDO; -.
DR   PDBsum; 2VHM; -.
DR   PDBsum; 2VHN; -.
DR   PDBsum; 2WWQ; -.
DR   PDBsum; 2Z4L; -.
DR   PDBsum; 2Z4N; -.
DR   PDBsum; 3BBX; -.
DR   PDBsum; 3DF2; -.
DR   PDBsum; 3DF4; -.
DR   PDBsum; 3E1B; -.
DR   PDBsum; 3E1D; -.
DR   PDBsum; 3FIK; -.
DR   PDBsum; 3I1N; -.
DR   PDBsum; 3I1P; -.
DR   PDBsum; 3I1R; -.
DR   PDBsum; 3I1T; -.
DR   PDBsum; 3I20; -.
DR   PDBsum; 3I22; -.
DR   PDBsum; 3IZT; -.
DR   PDBsum; 3IZU; -.
DR   PDBsum; 3J01; -.
DR   PDBsum; 3J0T; -.
DR   PDBsum; 3J0W; -.
DR   PDBsum; 3J0Y; -.
DR   PDBsum; 3J11; -.
DR   PDBsum; 3J12; -.
DR   PDBsum; 3J14; -.
DR   PDBsum; 3J19; -.
DR   PDBsum; 3J37; -.
DR   PDBsum; 3J4X; -.
DR   PDBsum; 3J50; -.
DR   PDBsum; 3J51; -.
DR   PDBsum; 3J52; -.
DR   PDBsum; 3J54; -.
DR   PDBsum; 3J56; -.
DR   PDBsum; 3J58; -.
DR   PDBsum; 3J5A; -.
DR   PDBsum; 3J5C; -.
DR   PDBsum; 3J5E; -.
DR   PDBsum; 3J5G; -.
DR   PDBsum; 3J5I; -.
DR   PDBsum; 3J5K; -.
DR   PDBsum; 3J5L; -.
DR   PDBsum; 3J5O; -.
DR   PDBsum; 3J5U; -.
DR   PDBsum; 3J5W; -.
DR   PDBsum; 3KCR; -.
DR   PDBsum; 3OAS; -.
DR   PDBsum; 3OAT; -.
DR   PDBsum; 3OFC; -.
DR   PDBsum; 3OFD; -.
DR   PDBsum; 3OFQ; -.
DR   PDBsum; 3OFR; -.
DR   PDBsum; 3OFZ; -.
DR   PDBsum; 3OG0; -.
DR   PDBsum; 3ORB; -.
DR   PDBsum; 3R8S; -.
DR   PDBsum; 3R8T; -.
DR   PDBsum; 3SGF; -.
DR   PDBsum; 3UOS; -.
DR   PDBsum; 4CSU; -.
DR   PDBsum; 4GAR; -.
DR   PDBsum; 4GAU; -.
DR   PDBsum; 4KIX; -.
DR   PDBsum; 4KIZ; -.
DR   PDBsum; 4KJ1; -.
DR   PDBsum; 4KJ3; -.
DR   PDBsum; 4KJ5; -.
DR   PDBsum; 4KJ7; -.
DR   PDBsum; 4KJ9; -.
DR   PDBsum; 4KJB; -.
DR   ProteinModelPortal; P60438; -.
DR   SMR; P60438; 1-209.
DR   DIP; DIP-10744N; -.
DR   IntAct; P60438; 144.
DR   MINT; MINT-1302843; -.
DR   STRING; 511145.b3320; -.
DR   ChEMBL; CHEMBL2363135; -.
DR   PaxDb; P60438; -.
DR   PRIDE; P60438; -.
DR   EnsemblBacteria; AAC76345; AAC76345; b3320.
DR   EnsemblBacteria; BAE77971; BAE77971; BAE77971.
DR   GeneID; 12932298; -.
DR   GeneID; 947817; -.
DR   KEGG; ecj:Y75_p3856; -.
DR   KEGG; eco:b3320; -.
DR   PATRIC; 32122072; VBIEscCol129921_3413.
DR   EchoBASE; EB0859; -.
DR   EcoGene; EG10866; rplC.
DR   eggNOG; COG0087; -.
DR   HOGENOM; HOG000100368; -.
DR   KO; K02906; -.
DR   OMA; SMQDATH; -.
DR   OrthoDB; EOG6WDSMH; -.
DR   PhylomeDB; P60438; -.
DR   BioCyc; EcoCyc:EG10866-MONOMER; -.
DR   BioCyc; ECOL316407:JW3282-MONOMER; -.
DR   EvolutionaryTrace; P60438; -.
DR   PRO; PR:P60438; -.
DR   Genevestigator; P60438; -.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IDA:EcoCyc.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_01325_B; Ribosomal_L3_B; 1.
DR   InterPro; IPR000597; Ribosomal_L3.
DR   InterPro; IPR019927; Ribosomal_L3_bac/org-type.
DR   InterPro; IPR019926; Ribosomal_L3_CS.
DR   InterPro; IPR009000; Transl_B-barrel.
DR   PANTHER; PTHR11229; PTHR11229; 1.
DR   Pfam; PF00297; Ribosomal_L3; 1.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   TIGRFAMs; TIGR03625; L3_bact; 1.
DR   PROSITE; PS00474; RIBOSOMAL_L3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Direct protein sequencing;
KW   Methylation; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW   RNA-binding; rRNA-binding.
FT   CHAIN         1    209       50S ribosomal protein L3.
FT                                /FTId=PRO_0000077098.
FT   MOD_RES      38     38       N6-succinyllysine.
FT   MOD_RES     150    150       N5-methylglutamine.
FT   STRAND        4      8
FT   STRAND       12     15
FT   TURN         17     19
FT   STRAND       21     28
FT   STRAND       32     38
FT   TURN         41     43
FT   STRAND       47     51
FT   HELIX        57     59
FT   HELIX        62     69
FT   STRAND       70     72
FT   STRAND       80     83
FT   TURN         91     93
FT   HELIX        98    101
FT   STRAND      103    105
FT   STRAND      106    112
FT   STRAND      115    118
FT   TURN        121    125
FT   STRAND      132    135
FT   STRAND      136    138
FT   STRAND      140    142
FT   TURN        150    152
FT   HELIX       155    157
FT   STRAND      162    164
FT   STRAND      166    168
FT   STRAND      177    181
FT   TURN        182    185
FT   STRAND      186    191
FT   STRAND      194    196
FT   STRAND      200    203
SQ   SEQUENCE   209 AA;  22244 MW;  EA8E10EDD2C0A8FD CRC64;
     MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV
     TKPEAGHFAK AGVEAGRGLW EFRLAEGEEF TVGQSISVEL FADVKKVDVT GTSKGKGFAG
     TVKRWNFRTQ DATHGNSLSH RVPGSIGQNQ TPGKVFKGKK MAGQMGNERV TVQSLDVVRV
     DAERNLLLVK GAVPGATGSD LIVKPAVKA
//
DBGET integrated database retrieval system