ID CANB1_HUMAN Reviewed; 170 AA.
AC P63098; B2RC10; B5MDU4; P06705; P15117; Q08044; Q53SL0;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 01-MAY-2013, entry version 102.
DE RecName: Full=Calcineurin subunit B type 1;
DE AltName: Full=Protein phosphatase 2B regulatory subunit 1;
DE AltName: Full=Protein phosphatase 3 regulatory subunit B alpha isoform 1;
GN Name=PPP3R1; Synonyms=CNA2, CNB;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2558868;
RA Guerini D., Krinks M.H., Sikela J.M., Hahn W.E., Klee C.B.;
RT "Isolation and sequence of a cDNA clone for human calcineurin B, the
RT Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein
RT phosphatase.";
RL DNA 8:675-682(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Blood;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 58-85; 98-117; 126-135 AND 148-164, AND MASS
RP SPECTROMETRY.
RC TISSUE=Fetal brain cortex;
RA Lubec G., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
RX PubMed=8524402; DOI=10.1038/378641a0;
RA Kissinger C.R., Parge H.E., Knighton D.R., Lewis C.T., Pelletier L.A.,
RA Tempczyk A., Kalish V.J., Tucker K.D., Showalter R.E., Moomaw E.W.,
RA Gastinel L.N., Habuka N., Chen X., Maldonado F., Barker J.E.,
RA Bacquet R., Villafranca J.E.;
RT "Crystal structures of human calcineurin and the human FKBP12-FK506-
RT calcineurin complex.";
RL Nature 378:641-644(1995).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH PPIA.
RX PubMed=12218175; DOI=10.1073/pnas.192206699;
RA Huai Q., Kim H.Y., Liu Y., Zhao Y., Mondragon A., Liu J.O., Ke H.;
RT "Crystal structure of calcineurin-cyclophilin-cyclosporin shows common
RT but distinct recognition of immunophilin-drug complexes.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:12037-12042(2002).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH PPIA.
RX PubMed=12357034; DOI=10.1073/pnas.212504399;
RA Jin L., Harrison S.C.;
RT "Crystal structure of human calcineurin complexed with cyclosporin A
RT and human cyclophilin.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13522-13526(2002).
CC -!- FUNCTION: Regulatory subunit of calcineurin, a calcium-dependent,
CC calmodulin stimulated protein phosphatase. Confers calcium
CC sensitivity.
CC -!- SUBUNIT: Composed of a catalytic subunit (A) and a regulatory
CC subunit (B).
CC -!- MISCELLANEOUS: This protein has four functional calcium-binding
CC sites.
CC -!- SIMILARITY: Belongs to the calcineurin regulatory subunit family.
CC -!- SIMILARITY: Contains 4 EF-hand domains.
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DR EMBL; M30773; AAB08721.1; -; mRNA.
DR EMBL; CR456938; CAG33219.1; -; mRNA.
DR EMBL; AK314893; BAG37407.1; -; mRNA.
DR EMBL; AC017083; AAY14715.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99878.1; -; Genomic_DNA.
DR EMBL; BC027913; AAH27913.1; -; mRNA.
DR IPI; IPI00027464; -.
DR PIR; A33391; A33391.
DR RefSeq; NP_000936.1; NM_000945.3.
DR UniGene; Hs.280604; -.
DR PDB; 1AUI; X-ray; 2.10 A; B=2-170.
DR PDB; 1M63; X-ray; 2.80 A; B/F=2-170.
DR PDB; 1MF8; X-ray; 3.10 A; B=1-170.
DR PDB; 2P6B; X-ray; 2.30 A; B/D=16-170.
DR PDB; 3LL8; X-ray; 2.00 A; B/D=16-170.
DR PDB; 4F0Z; X-ray; 1.70 A; B=1-170.
DR PDBsum; 1AUI; -.
DR PDBsum; 1M63; -.
DR PDBsum; 1MF8; -.
DR PDBsum; 2P6B; -.
DR PDBsum; 3LL8; -.
DR PDBsum; 4F0Z; -.
DR DisProt; DP00092; -.
DR ProteinModelPortal; P63098; -.
DR DIP; DIP-6096N; -.
DR IntAct; P63098; 3.
DR MINT; MINT-1339650; -.
DR STRING; 9606.ENSP00000234310; -.
DR PhosphoSite; P63098; -.
DR DMDM; 52000904; -.
DR OGP; P63098; -.
DR PaxDb; P63098; -.
DR PRIDE; P63098; -.
DR Ensembl; ENST00000234310; ENSP00000234310; ENSG00000221823.
DR GeneID; 5534; -.
DR KEGG; hsa:5534; -.
DR UCSC; uc002sei.1; human.
DR CTD; 5534; -.
DR GeneCards; GC02M068358; -.
DR HGNC; HGNC:9317; PPP3R1.
DR HPA; CAB005610; -.
DR MIM; 601302; gene.
DR neXtProt; NX_P63098; -.
DR PharmGKB; PA33681; -.
DR eggNOG; COG5126; -.
DR HOVERGEN; HBG105307; -.
DR KO; K06268; -.
DR Pathway_Interaction_DB; tcrcalciumpathway; Calcium signaling in the CD4+ TCR pathway.
DR Pathway_Interaction_DB; cd8tcrdownstreampathway; Downstream signaling in naive CD8+ T cells.
DR Pathway_Interaction_DB; il12_stat4pathway; IL12 signaling mediated by STAT4.
DR Pathway_Interaction_DB; il12_2pathway; IL12-mediated signaling events.
DR Pathway_Interaction_DB; nfat_3pathway; Role of Calcineurin-dependent NFAT signaling in lymphocytes.
DR Reactome; REACT_111102; Signal Transduction.
DR Reactome; REACT_118664; Calcineurin Dephosphorylates NFATC1/2/3.
DR Reactome; REACT_578; Apoptosis.
DR BindingDB; P63098; -.
DR ChEMBL; CHEMBL2082; -.
DR ChiTaRS; PPP3R1; human.
DR DrugBank; DB00337; Pimecrolimus.
DR EvolutionaryTrace; P63098; -.
DR GenomeRNAi; 5534; -.
DR NextBio; 21438; -.
DR ArrayExpress; P63098; -.
DR Bgee; P63098; -.
DR CleanEx; HS_PPP3R1; -.
DR Genevestigator; P63098; -.
DR GermOnline; ENSG00000115953; Homo sapiens.
DR GO; GO:0005955; C:calcineurin complex; NAS:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005509; F:calcium ion binding; NAS:UniProtKB.
DR GO; GO:0004723; F:calcium-dependent protein serine/threonine phosphatase activity; NAS:UniProtKB.
DR GO; GO:0005516; F:calmodulin binding; NAS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; TAS:Reactome.
DR Gene3D; 1.10.238.10; -; 1.
DR InterPro; IPR011992; EF-hand-like_dom.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF_hand_5; 1.
DR Pfam; PF00036; efhand; 2.
DR SMART; SM00054; EFh; 4.
DR PROSITE; PS00018; EF_HAND_1; 4.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Complete proteome; Direct protein sequencing;
KW Lipoprotein; Metal-binding; Myristate; Phosphoprotein;
KW Reference proteome; Repeat.
FT INIT_MET 1 1 Removed (By similarity).
FT CHAIN 2 170 Calcineurin subunit B type 1.
FT /FTId=PRO_0000073484.
FT DOMAIN 18 51 EF-hand 1.
FT DOMAIN 50 85 EF-hand 2.
FT DOMAIN 87 122 EF-hand 3.
FT DOMAIN 128 163 EF-hand 4.
FT CA_BIND 31 42 1.
FT CA_BIND 63 74 2.
FT CA_BIND 100 111 3.
FT CA_BIND 141 152 4.
FT MOD_RES 106 106 Phosphotyrosine (By similarity).
FT LIPID 2 2 N-myristoyl glycine (By similarity).
FT HELIX 18 30
FT STRAND 35 38
FT HELIX 40 43
FT HELIX 47 49
FT HELIX 55 62
FT STRAND 67 70
FT HELIX 72 79
FT HELIX 80 82
FT HELIX 88 99
FT STRAND 104 107
FT HELIX 109 120
FT HELIX 121 123
FT HELIX 126 140
FT STRAND 144 148
FT HELIX 150 157
FT HELIX 158 160
FT HELIX 162 165
SQ SEQUENCE 170 AA; 19300 MW; C904715DC0386056 CRC64;
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID
IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV
GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
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