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Database: UniProt
Entry: P65906
LinkDB: P65906
Original site: P65906 
ID   PYRC_STAAN              Reviewed;         424 AA.
AC   P65906; Q99UR7;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   26-NOV-2014, entry version 74.
DE   RecName: Full=Dihydroorotase {ECO:0000255|HAMAP-Rule:MF_00220};
DE            Short=DHOase {ECO:0000255|HAMAP-Rule:MF_00220};
DE            EC=3.5.2.3 {ECO:0000255|HAMAP-Rule:MF_00220};
GN   Name=pyrC {ECO:0000255|HAMAP-Rule:MF_00220}; OrderedLocusNames=SA1044;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/S0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I.,
RA   Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K.,
RA   Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M.,
RA   Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M.,
RA   Ogasawara N., Hayashi H., Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus
RT   aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RA   Stenz L.;
RT   "Shotgun proteomic analysis of total protein extract of S. aureus S30
RT   versus N315.";
RL   Submitted (NOV-2005) to UniProtKB.
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of
RT   S. aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate. {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00220};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_00220};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00220}.
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DR   EMBL; BA000018; BAB42296.1; -; Genomic_DNA.
DR   PIR; D89892; D89892.
DR   RefSeq; NP_374317.1; NC_002745.2.
DR   ProteinModelPortal; P65906; -.
DR   SMR; P65906; 1-423.
DR   STRING; 158879.SA1044; -.
DR   PRIDE; P65906; -.
DR   EnsemblBacteria; BAB42296; BAB42296; BAB42296.
DR   GeneID; 1123875; -.
DR   KEGG; sau:SA1044; -.
DR   PATRIC; 19574306; VBIStaAur116463_1122.
DR   eggNOG; COG0044; -.
DR   HOGENOM; HOG000219142; -.
DR   KO; K01465; -.
DR   OMA; EDIHTGT; -.
DR   OrthoDB; EOG6KHFW6; -.
DR   BioCyc; SAUR158879:GJCB-1104-MONOMER; -.
DR   UniPathway; UPA00070; UER00117.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_00220_B; PyrC_type2_B; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004722; DHOase.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   TIGRFAMs; TIGR00857; pyrC_multi; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    424       Dihydroorotase.
FT                                /FTId=PRO_0000147248.
FT   METAL        58     58       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL        60     60       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       177    177       Zinc 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       230    230       Zinc 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       303    303       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
SQ   SEQUENCE   424 AA;  46372 MW;  8EC9B615ED8FFAB2 CRC64;
     MKLIKNGKVL QNGELQQADI LIDGKVIKQI APAIEPSNGV DIIDAKGHFV SPGFVDVHVH
     LREPGGEYKE TIETGTKAAA RGGFTTVCPM PNTRPVPDSV EHFEALQKLI DDNAQVRVLP
     YASITTRQLG KELVDFPALV KEGAFAFTDD GVGVQTASMM YEGMIEAAKV NKAIVAHCED
     NSLIYGGAMH EGKRSKELGI PGIPNICESV QIARDVLLAE AAGCHYHVCH VSTKESVRVI
     RDAKRAGIHV TAEVTPHHLL LTEDDIPGNN AIYKMNPPLR STEDREALLE GLLDGTIDCI
     ATDHAPHARD EKAQPMEKAP FGIVGSETAF PLLYTHFVKN GDWTLQQLVD YLTIKPCETF
     NLEYGTLKEN GYADLTIIDL DSEQEIKGED FLSKADNTPF IGYKVYGNPI LTMVEGEVKF
     EGDK
//
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