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Database: UniProt
Entry: P69787
LinkDB: P69787
Original site: P69787 
ID   PTGCB_ECOL6             Reviewed;         477 AA.
AC   P69787; P05053;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   03-SEP-2014, entry version 72.
DE   RecName: Full=PTS system glucose-specific EIICB component;
DE   AltName: Full=EIICB-Glc;
DE            Short=EII-Glc;
DE   Includes:
DE     RecName: Full=Glucose permease IIC component;
DE     AltName: Full=PTS system glucose-specific EIIC component;
DE   Includes:
DE     RecName: Full=Glucose-specific phosphotransferase enzyme IIB component;
DE              EC=2.7.1.69;
DE     AltName: Full=PTS system glucose-specific EIIB component;
GN   Name=ptsG; OrderedLocusNames=c1373;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P.,
RA   Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D.,
RA   Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T.,
RA   Mobley H.L.T., Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence
RT   of uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (sugar PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitantly with their translocation across the cell
CC       membrane. This system is involved in glucose transport. This
CC       enzyme is also a chemoreceptor monitoring the environment for
CC       changes in sugar concentration (By similarity).
CC   -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC       protein (By similarity).
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC       and contains the specific substrate-binding site.
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIB type-1 domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIC type-1 domain.
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DR   EMBL; AE014075; AAN79843.1; -; Genomic_DNA.
DR   RefSeq; NP_753283.1; NC_004431.1.
DR   ProteinModelPortal; P69787; -.
DR   SMR; P69787; 396-476.
DR   STRING; 199310.c1373; -.
DR   PRIDE; P69787; -.
DR   EnsemblBacteria; AAN79843; AAN79843; c1373.
DR   GeneID; 1035072; -.
DR   KEGG; ecc:c1373; -.
DR   PATRIC; 18280755; VBIEscCol75197_1302.
DR   HOGENOM; HOG000250993; -.
DR   KO; K02778; -.
DR   KO; K02779; -.
DR   OMA; MQIGEFT; -.
DR   OrthoDB; EOG6FFS9V; -.
DR   BioCyc; ECOL199310:C1373-MONOMER; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005355; F:glucose transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1360.60; -; 1.
DR   InterPro; IPR018113; PTrfase_EIIB_Cys.
DR   InterPro; IPR003352; PTS_EIIC.
DR   InterPro; IPR013013; PTS_EIIC_1.
DR   InterPro; IPR001996; PTS_IIB_1.
DR   InterPro; IPR011299; PTS_IIBC_glc.
DR   InterPro; IPR004719; PTS_maltose/Glc_sub_IIC.
DR   Pfam; PF00367; PTS_EIIB; 1.
DR   Pfam; PF02378; PTS_EIIC; 1.
DR   SUPFAM; SSF55604; SSF55604; 1.
DR   TIGRFAMs; TIGR00826; EIIB_glc; 1.
DR   TIGRFAMs; TIGR00852; pts-Glc; 1.
DR   TIGRFAMs; TIGR02002; PTS-II-BC-glcB; 1.
DR   PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR   PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR   PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Complete proteome; Kinase;
KW   Membrane; Phosphotransferase system; Sugar transport; Transferase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    477       PTS system glucose-specific EIICB
FT                                component.
FT                                /FTId=PRO_0000186529.
FT   TOPO_DOM      1     14       Cytoplasmic (Potential).
FT   TRANSMEM     15     35       Helical; (Potential).
FT   TOPO_DOM     36     50       Periplasmic (Potential).
FT   TRANSMEM     51     71       Helical; (Potential).
FT   TOPO_DOM     72     79       Cytoplasmic (Potential).
FT   TRANSMEM     80    100       Helical; (Potential).
FT   TOPO_DOM    101    111       Periplasmic (Potential).
FT   TRANSMEM    112    132       Helical; (Potential).
FT   TOPO_DOM    133    151       Cytoplasmic (Potential).
FT   TRANSMEM    152    172       Helical; (Potential).
FT   TOPO_DOM    173    190       Periplasmic (Potential).
FT   TRANSMEM    191    211       Helical; (Potential).
FT   TOPO_DOM    212    249       Cytoplasmic (Potential).
FT   TRANSMEM    250    270       Helical; (Potential).
FT   TOPO_DOM    271    279       Periplasmic (Potential).
FT   TRANSMEM    280    300       Helical; (Potential).
FT   TOPO_DOM    301    309       Cytoplasmic (Potential).
FT   TRANSMEM    310    330       Helical; (Potential).
FT   TOPO_DOM    331    355       Periplasmic (Potential).
FT   TRANSMEM    356    376       Helical; (Potential).
FT   TOPO_DOM    377    477       Cytoplasmic (Potential).
FT   DOMAIN        1    388       PTS EIIC type-1.
FT   DOMAIN      399    477       PTS EIIB type-1.
FT   ACT_SITE    421    421       Phosphocysteine intermediate; for EIIB
FT                                activity (By similarity).
SQ   SEQUENCE   477 AA;  50677 MW;  D97A80FD64B74F73 CRC64;
     MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE AGGSVFANMP
     LIFAIGVALG FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV LHLPAEEIAS KHLADTGVLG
     GIISGAIAAY MFNRFYRIKL PEYLGFFAGK RFVPIISGLA AIFTGVVLSF IWPPIGSAIQ
     TFSQWAAYQN PVVAFGIYGF IERCLVPFGL HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY
     MAGDPTAGKL SGGFLFKMYG LPAAAIAIWH SAKPENRAKV GGIMISAALT SFLTGITEPI
     EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN SSKLWLFPIV
     GIGYAIVYYT IFRVLIKALD LKTPGREDAT EDAKATGTSE MAPALVAAFG GKENITNLDA
     CITRLRVSVA DVSKVDQAGL KKLGAAGVVV AGSGVQAIFG TKSDNLKTEM DEYIRNH
//
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