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Database: UniProt
Entry: P69827
LinkDB: P69827
Original site: P69827 
ID   PTMCB_ECO57             Reviewed;         462 AA.
AC   P69827; P32059;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   29-OCT-2014, entry version 66.
DE   RecName: Full=PTS system mannitol-specific cryptic EIICB component;
DE   AltName: Full=EIICB-Mtl;
DE            Short=EII-Mtl;
DE   Includes:
DE     RecName: Full=Mannitol permease IIC component;
DE     AltName: Full=PTS system mannitol-specific EIIC component;
DE   Includes:
DE     RecName: Full=Mannitol-specific phosphotransferase enzyme IIB component;
DE              EC=2.7.1.69;
DE     AltName: Full=PTS system mannitol-specific EIIB component;
GN   Name=cmtA; OrderedLocusNames=Z4277, ECs3808;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (sugar PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitantly with their translocation across the cell
CC       membrane. This system is involved in mannitol transport (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC       {ECO:0000255|PROSITE-ProRule:PRU00422}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00427}; Multi-pass membrane protein
CC       {ECO:0000255|PROSITE-ProRule:PRU00427}.
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC       and contains the specific substrate-binding site.
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIB type-2 domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00422}.
CC   -!- SIMILARITY: Contains 1 PTS EIIC type-2 domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00427}.
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DR   EMBL; AE005174; AAG58063.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37231.1; -; Genomic_DNA.
DR   PIR; H91104; H91104.
DR   RefSeq; NP_289504.1; NC_002655.2.
DR   RefSeq; NP_311835.1; NC_002695.1.
DR   ProteinModelPortal; P69827; -.
DR   SMR; P69827; 368-462.
DR   STRING; 155864.Z4277; -.
DR   EnsemblBacteria; AAG58063; AAG58063; Z4277.
DR   EnsemblBacteria; BAB37231; BAB37231; BAB37231.
DR   GeneID; 916373; -.
DR   GeneID; 958491; -.
DR   KEGG; ece:Z4277; -.
DR   KEGG; ecs:ECs3808; -.
DR   PATRIC; 18357107; VBIEscCol44059_3732.
DR   eggNOG; COG2213; -.
DR   HOGENOM; HOG000252814; -.
DR   KO; K02799; -.
DR   KO; K02800; -.
DR   OMA; DIVVTHE; -.
DR   OrthoDB; EOG6FBWWQ; -.
DR   BioCyc; ECOL386585:GJFA-3775-MONOMER; -.
DR   BioCyc; ECOO157:CMTA-MONOMER; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0022872; F:protein-N(PI)-phosphohistidine-mannitol phosphotransferase system transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.10370; -; 1.
DR   InterPro; IPR013011; PTS_EIIB_2.
DR   InterPro; IPR003501; PTS_EIIB_2/3.
DR   InterPro; IPR029503; PTS_EIIB_mannitol.
DR   InterPro; IPR003352; PTS_EIIC.
DR   InterPro; IPR013014; PTS_EIIC_2.
DR   InterPro; IPR004718; PTS_IIC_mtl.
DR   Pfam; PF02378; PTS_EIIC; 1.
DR   Pfam; PF02302; PTS_IIB; 1.
DR   SUPFAM; SSF52794; SSF52794; 1.
DR   TIGRFAMs; TIGR00851; mtlA; 1.
DR   PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
DR   PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Complete proteome; Kinase;
KW   Membrane; Phosphotransferase system; Sugar transport; Transferase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    462       PTS system mannitol-specific cryptic
FT                                EIICB component.
FT                                /FTId=PRO_0000186680.
FT   TOPO_DOM      1     25       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM     26     45       Helical. {ECO:0000305}.
FT   TOPO_DOM     46     51       Periplasmic. {ECO:0000255}.
FT   TRANSMEM     52     70       Helical. {ECO:0000305}.
FT   TOPO_DOM     71    135       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    136    155       Helical. {ECO:0000305}.
FT   TOPO_DOM    156    166       Periplasmic. {ECO:0000255}.
FT   TRANSMEM    167    185       Helical. {ECO:0000305}.
FT   TOPO_DOM    186    273       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    274    294       Helical. {ECO:0000305}.
FT   TOPO_DOM    295    316       Periplasmic. {ECO:0000255}.
FT   TRANSMEM    317    336       Helical. {ECO:0000305}.
FT   TOPO_DOM    337    462       Cytoplasmic. {ECO:0000255}.
FT   DOMAIN       13    344       PTS EIIC type-2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00427}.
FT   DOMAIN      371    461       PTS EIIB type-2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00422}.
FT   ACT_SITE    377    377       Phosphocysteine intermediate; for EIIB
FT                                activity. {ECO:0000250}.
SQ   SEQUENCE   462 AA;  48971 MW;  83F2D203D9E5A4C4 CRC64;
     MENKSARAKV QAFGGFLTAM VIPNIGAFIA WGFITALFIP TGWLPNEHFA KIVGPMITYL
     LPVMIGSTGG HLVGGKRGAV MGGIGTIGVI VGAEIPMFLG SMIMGPLGGL VIKYVDKALE
     KRIPAGFEMV INNFSLGIAG MLLCLLGFEV IGPAVLIANT FVKECIEALV HAGYLPLLSV
     INEPAKVLFL NNAIDQGVYY PLGMQQASVN GKSIFFMVAS NPGPGLGLLL AFTLFGKGMS
     KRSAPGAMII HFLGGIHELY FPYVLMKPLT IIAMIAGGMS GTWMFNLLDG GLVAGPSPGS
     IFAYLALTPK GSFLATIAGV TVGTLVSFAI TSLILKMEKT VETESEDEFA QSANAVKAMK
     QEGAFSLSRV KRIAFVCDAG MGSSAMGATT FRKRLEKAGL AIEVKHYAIE NVPADADIVV
     THASLEGRVK RVTDKPLILI NNYIGDPKLD TLFNQLTAEH KH
//
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