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Database: UniProt
Entry: P73493
LinkDB: P73493
Original site: P73493 
ID   GSHB_SYNY3              Reviewed;         320 AA.
AC   P73493;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   29-OCT-2014, entry version 98.
DE   RecName: Full=Glutathione synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            EC=6.3.2.3 {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=GSH synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSH-S {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSHase {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=Glutathione synthase {ECO:0000255|HAMAP-Rule:MF_00162};
GN   Name=gshB {ECO:0000255|HAMAP-Rule:MF_00162};
GN   OrderedLocusNames=slr1238;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA   Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA   Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA   Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT   entire genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione. {ECO:0000255|HAMAP-Rule:MF_00162}.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit.
CC       {ECO:0000250}.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00162}.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
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DR   EMBL; BA000022; BAA17533.1; -; Genomic_DNA.
DR   PIR; S77430; S77430.
DR   RefSeq; NP_440853.1; NC_000911.1.
DR   RefSeq; YP_005650912.1; NC_017277.1.
DR   RefSeq; YP_007450736.1; NC_020286.1.
DR   ProteinModelPortal; P73493; -.
DR   IntAct; P73493; 1.
DR   STRING; 1148.slr1238; -.
DR   PaxDb; P73493; -.
DR   EnsemblBacteria; BAA17533; BAA17533; BAA17533.
DR   GeneID; 954156; -.
DR   KEGG; syn:slr1238; -.
DR   KEGG; syy:SYNGTS_0959; -.
DR   KEGG; syz:MYO_19660; -.
DR   PATRIC; 23838946; VBISynSp132158_1038.
DR   eggNOG; COG0189; -.
DR   HOGENOM; HOG000265022; -.
DR   InParanoid; P73493; -.
DR   KO; K01920; -.
DR   OMA; ERERQIC; -.
DR   OrthoDB; EOG6WMHWB; -.
DR   PhylomeDB; P73493; -.
DR   UniPathway; UPA00142; UER00210.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1    320       Glutathione synthetase.
FT                                /FTId=PRO_0000197490.
FT   DOMAIN      133    317       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_00162}.
FT   NP_BIND     159    215       ATP. {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       288    288       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       290    290       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
SQ   SEQUENCE   320 AA;  35069 MW;  6C7BD439A23D185A CRC64;
     MKLAFIIDPL EKLDPGHDST VAIMEAAQKL GHEVFVTSVG DLAVINGQAW AKLAAVRLQP
     VILVDGHWQV SQPWSELSKS QWMALTECQA VFMRKDPPVT VQYLYATFIL ELLAPTKTMV
     INSPQGLREA NEKMYTLQFA AVMPPTVVSL DKGLIRQFLE EHGAAVLKPL GGKAGEGILF
     LDPGDRNFNS LVEISTQHGK EPVMVQRFLP EAKEGDKRII LLDGDPIGAV NRIPSGAEFR
     GNMAVGGQVA PTTITSREIE ICALLKPKLQ ADGLYFVGID VIGGYLTEVN VTSPTGIREI
     DRLEGVRLGE KVICWLEKQF
//
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