ID PTMCB_MYCPN Reviewed; 488 AA.
AC P75146;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 29-MAY-2013, entry version 91.
DE RecName: Full=PTS system mannitol-specific EIICB component;
DE AltName: Full=EIICB-Mtl;
DE Short=EII-Mtl;
DE Includes:
DE RecName: Full=Mannitol permease IIC component;
DE AltName: Full=PTS system mannitol-specific EIIC component;
DE Includes:
DE RecName: Full=Mannitol-specific phosphotransferase enzyme IIB component;
DE EC=2.7.1.69;
DE AltName: Full=PTS system mannitol-specific EIIB component;
GN Name=mtlA; OrderedLocusNames=MPN_651; ORFNames=MP191;
OS Mycoplasma pneumoniae (strain ATCC 29342 / M129).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=272634;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29342 / M129;
RX PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C.,
RA Herrmann R.;
RT "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT pneumoniae.";
RL Nucleic Acids Res. 24:4420-4449(1996).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC phosphotransferase system (sugar PTS), a major carbohydrate active
CC -transport system, catalyzes the phosphorylation of incoming sugar
CC substrates concomitantly with their translocation across the cell
CC membrane. This system is involved in mannitol transport (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein
CC (Potential).
CC -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC and contains the specific substrate-binding site.
CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC cysteinyl or histidyl residue, depending on the transported sugar.
CC Then, it transfers the phosphoryl group to the sugar substrate
CC concomitantly with the sugar uptake processed by the EIIC domain.
CC -!- SIMILARITY: Contains 1 PTS EIIB type-2 domain.
CC -!- SIMILARITY: Contains 1 PTS EIIC type-2 domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB95839.1; Type=Frameshift; Positions=55, 68;
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DR EMBL; U00089; AAB95839.1; ALT_FRAME; Genomic_DNA.
DR PIR; S73517; S73517.
DR RefSeq; NP_110340.1; NC_000912.1.
DR ProteinModelPortal; P75146; -.
DR STRING; 272634.MPN651; -.
DR EnsemblBacteria; AAB95839; AAB95839; MPN_651.
DR GeneID; 877002; -.
DR KEGG; mpn:MPN651; -.
DR PATRIC; 20022785; VBIMycPne110_0715.
DR eggNOG; COG2213; -.
DR KO; K02799; -.
DR KO; K02800; -.
DR OMA; ITHKDLT; -.
DR ProtClustDB; CLSK2459739; -.
DR BioCyc; MPNE272634:GJ6Z-697-MONOMER; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:EC.
DR GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.10370; -; 1.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013014; PTS_EIIC_2.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
DR PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Complete proteome; Kinase; Membrane;
KW Phosphotransferase system; Reference proteome; Sugar transport;
KW Transferase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 488 PTS system mannitol-specific EIICB
FT component.
FT /FTId=PRO_0000186619.
FT TRANSMEM 22 42 Helical; (Potential).
FT TRANSMEM 51 71 Helical; (Potential).
FT TRANSMEM 80 100 Helical; (Potential).
FT TRANSMEM 103 123 Helical; (Potential).
FT TRANSMEM 151 171 Helical; (Potential).
FT TRANSMEM 187 207 Helical; (Potential).
FT TRANSMEM 227 247 Helical; (Potential).
FT TRANSMEM 277 297 Helical; (Potential).
FT TRANSMEM 299 319 Helical; (Potential).
FT TRANSMEM 335 355 Helical; (Potential).
FT DOMAIN 15 362 PTS EIIC type-2.
FT DOMAIN 397 488 PTS EIIB type-2.
FT ACT_SITE 403 403 Phosphocysteine intermediate; for EIIB
FT activity (By similarity).
SQ SEQUENCE 488 AA; 53440 MW; E4CDE1411110C9C4 CRC64;
MRKKLAKVKV HIQSLDSLLS SMTMPIIGIF IAWGLLASFF IPSGWTPDKN LALMVGIGIQ
YVIPTIIXFF GGKKIYEIRG GVIAVIIAIA VIAAGQTEAF TKIVGQKSVM FLGVMIFGPI
AALILKHTEK FWIHRIKSGF EMLVNNFYLG FLGFALIFPS FYLSIYLIGY IQLGLKLLVE
IMQQYKLYPI AAIVIEPAKV LFLNNAINHG VLTPLGLQQV RDSGKSILFL LESNPGPGLG
LLVAFLIFFF KRDKKLSSNA ASSSPIHLFG GIHEVYFPFV LLKPVLILAT IAVGVVGNGI
LQIFNAGTIA PVSPGSVIAG FLQINKTPLD VAGYALALVL SAVTSLLISL LLLSLTRKKQ
LKTLQEAQAQ VAEMKQTPAK KPRQKDTPAI ATKIDFSQVT FVCDAGMGSS TMGAAIFRKE
LKNQNIEDIT VINKAIVDLK DEKVIITISQ LYDRVKAKRA DATIYTINQF LDKQGYLTII
EKIKNEKN
//
