ID PAFA_CAEEL Reviewed; 388 AA.
AC Q22943;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 27-MAR-2024, entry version 142.
DE RecName: Full=Platelet-activating factor acetylhydrolase homolog 2;
DE EC=3.1.1.47;
GN Name=paf-2; ORFNames=C52B9.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RA Inoue T., Suzuki Y., Aoki J., Sugimoto A., Yamamoto M., Tsujimoto M.,
RA Inoue K., Arai H.;
RT "A short-chain acyl hydrolase is required for early epithelial
RT morphogenesis in Caenorhabditis elegans.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-O-
CC alkyl-sn-glycero-3-phosphocholine + acetate + H(+);
CC Xref=Rhea:RHEA:17777, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30089, ChEBI:CHEBI:30909, ChEBI:CHEBI:36707; EC=3.1.1.47;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q22943-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q22943-2; Sequence=VSP_003132, VSP_003133;
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
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DR EMBL; AF386745; AAL57290.1; -; mRNA.
DR EMBL; FO080644; CCD65415.1; -; Genomic_DNA.
DR EMBL; FO080644; CCD65416.1; -; Genomic_DNA.
DR RefSeq; NP_741768.1; NM_171668.6. [Q22943-1]
DR RefSeq; NP_741769.1; NM_171669.3.
DR AlphaFoldDB; Q22943; -.
DR SMR; Q22943; -.
DR STRING; 6239.C52B9.7a.1; -.
DR ESTHER; caeel-pafa; PAF-Acetylhydrolase.
DR EPD; Q22943; -.
DR PaxDb; 6239-C52B9-7a; -.
DR PeptideAtlas; Q22943; -.
DR EnsemblMetazoa; C52B9.7a.1; C52B9.7a.1; WBGene00003907. [Q22943-1]
DR EnsemblMetazoa; C52B9.7a.2; C52B9.7a.2; WBGene00003907. [Q22943-1]
DR EnsemblMetazoa; C52B9.7b.1; C52B9.7b.1; WBGene00003907. [Q22943-2]
DR GeneID; 180704; -.
DR KEGG; cel:CELE_C52B9.7; -.
DR UCSC; C52B9.7a; c. elegans. [Q22943-1]
DR AGR; WB:WBGene00003907; -.
DR WormBase; C52B9.7a; CE30264; WBGene00003907; paf-2. [Q22943-1]
DR WormBase; C52B9.7b; CE30265; WBGene00003907; paf-2. [Q22943-2]
DR eggNOG; KOG3847; Eukaryota.
DR GeneTree; ENSGT00390000005233; -.
DR HOGENOM; CLU_022501_0_0_1; -.
DR InParanoid; Q22943; -.
DR OMA; DKWPIVV; -.
DR OrthoDB; 3079661at2759; -.
DR PhylomeDB; Q22943; -.
DR BRENDA; 3.1.1.47; 1045.
DR Reactome; R-CEL-418346; Platelet homeostasis.
DR PRO; PR:Q22943; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003907; Expressed in material anatomical entity and 5 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; ISS:WormBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:WormBase.
DR GO; GO:0003847; F:1-alkyl-2-acetylglycerophosphocholine esterase activity; IDA:WormBase.
DR GO; GO:0016042; P:lipid catabolic process; IDA:WormBase.
DR GO; GO:0016331; P:morphogenesis of embryonic epithelium; IMP:WormBase.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR016715; PAF_acetylhydro_eukaryote.
DR PANTHER; PTHR10272; PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE; 1.
DR PANTHER; PTHR10272:SF0; PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE; 1.
DR Pfam; PF03403; PAF-AH_p_II; 1.
DR PIRSF; PIRSF018169; PAF_acetylhydrolase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome.
FT CHAIN 1..388
FT /note="Platelet-activating factor acetylhydrolase homolog
FT 2"
FT /id="PRO_0000090381"
FT ACT_SITE 238
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 261
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 316
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT VAR_SEQ 187..190
FT /note="GKRV -> SVKV (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_003132"
FT VAR_SEQ 191..388
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_003133"
SQ SEQUENCE 388 AA; 43418 MW; 98B71A8ADA43E701 CRC64;
MGSYISSPQV LTRQVSGQFQ VGCKDLMIDG TVLGDRGLFM RLYFPTDSQA ADISSYPLWL
PKPQYAHGLG EYLGQSSQKM NVITSTVVGE KREDCIENAQ MSTKCDKWPI VVFSHGLGGS
RTFYSTYCTS LASHGYVVAA VEHKDHSACW TYQLTEKNGE LVEQPIKIKL IEKNEKNEFK
IRNQQVGKRV TECVKALNVL EQLNLGTVPE KVLIGNDYNW AQFKNKLVMS SASVIGHSFG
GATSLASSAY TTDFQKAIVF DGWMYPLDST QQEQAKQPTL FLNVGDWQWN ENLDVMKKII
SHNDGNLALT LNGAVHQCFS DFPFIFPSWL AKKFGVQGRT EPSLCMQAAI ELSLAFLENG
KDGAQKLKDE KFSSFISNEI YGREKYKL
//
