ID PALB_NEUCR Reviewed; 932 AA.
AC Q7RZP7; U9W3D3;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-FEB-2014, sequence version 3.
DT 24-JAN-2024, entry version 93.
DE RecName: Full=Calpain-like protease palB/cpr-8;
DE EC=3.4.22.-;
DE AltName: Full=Cysteine protease 8;
GN Name=cpr-8; Synonyms=rim13; ORFNames=NCU00317;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC factor pacc-1 in response to alkaline ambient pH. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase C2 family. PalB/RIM13 subfamily.
CC {ECO:0000305}.
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DR EMBL; CM002238; ESA43337.1; -; Genomic_DNA.
DR RefSeq; XP_011393811.1; XM_011395509.1.
DR AlphaFoldDB; Q7RZP7; -.
DR SMR; Q7RZP7; -.
DR STRING; 367110.Q7RZP7; -.
DR MEROPS; C02.008; -.
DR PaxDb; 5141-EFNCRP00000000354; -.
DR EnsemblFungi; ESA43337; ESA43337; NCU00317.
DR GeneID; 3873926; -.
DR KEGG; ncr:NCU00317; -.
DR VEuPathDB; FungiDB:NCU00317; -.
DR HOGENOM; CLU_006770_1_0_1; -.
DR InParanoid; Q7RZP7; -.
DR OrthoDB; 230045at2759; -.
DR Proteomes; UP000001805; Chromosome 3, Linkage Group III.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00044; CysPc; 1.
DR Gene3D; 2.60.120.380; -; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR022682; Calpain_domain_III.
DR InterPro; IPR022683; Calpain_III.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR PANTHER; PTHR46143; CALPAIN-7; 1.
DR PANTHER; PTHR46143:SF1; CALPAIN-7; 1.
DR Pfam; PF01067; Calpain_III; 1.
DR Pfam; PF00648; Peptidase_C2; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00720; calpain_III; 1.
DR SMART; SM00230; CysPc; 1.
DR SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 2.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
PE 3: Inferred from homology;
KW Hydrolase; Protease; Reference proteome; Thiol protease.
FT CHAIN 1..932
FT /note="Calpain-like protease palB/cpr-8"
FT /id="PRO_0000207744"
FT DOMAIN 96..419
FT /note="Calpain catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT REGION 890..932
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT ACT_SITE 346
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT ACT_SITE 366
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
SQ SEQUENCE 932 AA; 101464 MW; 70F6A8297F129747 CRC64;
MEARALEHER RLAESHGQEA LKHAIAAAEI YMRAAEKAAN PKDRNRLQRK CSDLIALGER
LKANAKSAAT SARSPVPEST RTLTIAEKTL LLKSSKLHGN IFPPWEKTPA SDEFAASKAA
DGCFTDHSPF TLSPEQQDIF AGWKRPHEIF MDVPERGVDV FMTATESIDL GQDLATDCSV
VASLCAAVRQ FGPRTGSLLS SLMYPYDDDV KRPAVSQNGK YIFRMYFNGC WRKVLIDDRL
PTSSSERTLY VVDRRNPYLI WPALIEKAYL KIRGGYDFPG SNSGTDLHAL TGWIPEQIFL
QTDDIELNET WSRIKTAYEQ GNALVTLGTG KFSREEERTL GLVREHDYAV LDLRNDGNNR
LFLVKNPWRD SLVWTGVGST ATSSTDRSGS PEESMSNTFW MTFEDVLQHF DSLYVNWSPS
LFRFRQDHHF TWTIPPKAEE LVFTQNPQYS ILSHTGSPVW ILLNRHWQDS ELDILRERKQ
EHDFHQPLKS LGYMSLSLFA SHPPGTRIPL SEGSHHALHQ GPFVDSPNTL LRYSPTPGVA
QTLVIAQSDL PLPSYSFTLS FFSNSPLTIS PASDPLPYNE TITGAWTRRT AGGSTVYPSY
VTNPQYALTL TRPSPLSLVL STERADNLPV HIAVLYSNGG QRVTAVVGRD LICSSAEYQR
GCTFASTLPS SNTSNVTSSV ASNNHGHTSS SLIDPGTYTI VLSTYEPGQT GRYSLRVSAA
CPFTIEPILS DAAGRLRTPA PSPATFRQGE GRVRARVDVA RLTRASVLAR SVKTGSTTQK
TAMVRVALEL GTIEGRKRVL ASTASGGGGE LAASLSNLSL SERLGGIGGI GGGHIHGGQG
TTEGDGYSAK GEFADASLGL RTREVDLDPD VIRVYGGLWL VVEQIGGGGQ GHVTEGSDDD
GGGGGGGGGG VHVEISSDGV VSIGEWEVAD ED
//
