ID PKG1_ADEM1 Reviewed; 449 AA.
AC P12539;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 2.
DT 14-DEC-2022, entry version 74.
DE RecName: Full=Packaging protein 1 {ECO:0000255|HAMAP-Rule:MF_04057};
DE AltName: Full=Packaging protein IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
GN Name=IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
OS Murine adenovirus A serotype 1 (MAdV-1) (Murine adenovirus 1).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus; Murine mastadenovirus A.
OX NCBI_TaxID=10530;
OH NCBI_TaxID=10090; Mus musculus (Mouse).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-90.
RX PubMed=2374728; DOI=10.1093/nar/18.13.4003;
RA Kring S.C., Spindler K.R.;
RT "Sequence of mouse adenovirus type 1 DNA encoding the amino terminus of
RT protein IVa2.";
RL Nucleic Acids Res. 18:4003-4003(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 91-449.
RX PubMed=2543128; DOI=10.1016/0042-6822(89)90444-3;
RA Ball A.O., Beard C.W., Redick S.D., Spindler K.R.;
RT "Genome organization of mouse adenovirus type 1 early region 1: a novel
RT transcription map.";
RL Virology 170:523-536(1989).
CC -!- FUNCTION: Component of the packaging machinery which encapsidates the
CC viral DNA into preformed capsids and transcriptional activator of the
CC viral major late promoter (MLP). Binds, along with packaging proteins 2
CC and 3, to the specific packaging sequence on the left end of viral
CC genomic DNA and displays ATPase activity thereby providing the power
CC stroke of the packaging machinery. The activity of packaging protein
CC IVa2 is stimulated by protein 33K which acts as a terminase. May be the
CC protein that pumps DNA into the capsid powered by ATP hydrolysis.
CC Specifically binds to the 5'-CG-3' nucleotides of the repeats making up
CC the packaging sequence. Component of the DEF-A and DEF-B transcription
CC factors that bind downstream elements of the major late promoter (MLP),
CC and stimulate transcription from the MLP after initiation of viral DNA
CC replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-
CC B is a homodimer of packaging protein 1. {ECO:0000255|HAMAP-
CC Rule:MF_04057}.
CC -!- SUBUNIT: Homodimer. Part of a genome packaging complex composed of
CC packaging proteins 1, 2 and 3; this complex specifically binds to the
CC packaging sequence on the left end of viral genomic DNA and performs
CC packaging of the viral genome. Interacts with protein 33K.
CC {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC nucleus, host nucleoplasm {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC nucleus, host nucleolus {ECO:0000255|HAMAP-Rule:MF_04057}. Note=Located
CC at a unique vertex of the capsid. Present in about 6-8 copies per
CC virion. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- INDUCTION: Expressed in the intermediate phase of the viral replicative
CC cycle. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- SIMILARITY: Belongs to the adenoviridae packaging protein 1 family.
CC {ECO:0000255|HAMAP-Rule:MF_04057}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M22245; AAA42431.1; -; Genomic_DNA.
DR PIR; S12848; S12848.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0044095; C:host cell nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0039708; P:nuclear capsid assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0098035; P:viral DNA genome packaging via site-specific sequence recognition; IEA:UniProtKB-UniRule.
DR GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04057; ADV_PKG1; 1.
DR InterPro; IPR003389; Adeno_IVa2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02456; Adeno_IVa2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; DNA-binding; Host nucleus; Nucleotide-binding;
KW Phosphoprotein; Transcription; Transcription regulation;
KW Viral genome packaging; Viral release from host cell; Virion.
FT CHAIN 1..449
FT /note="Packaging protein 1"
FT /id="PRO_0000221892"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..449
FT /note="DNA-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
FT COMPBIAS 1..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 168..175
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
SQ SEQUENCE 449 AA; 50589 MW; 5015B45199DB5E5C CRC64;
MESRGKHRLK KNGESKENLG EHEQARIGFH RNGDSADSLS SPVAEPNFSS PGGRSSNSIL
HCPPQSPEQG NNLDCLVGEE LQFLWPRLQC LQHTLSNLPL SEGLKPLMNF ETVEDLAALA
GKGLLQELKH DYEILCKSLN SAAPLLSPEG NCESLNYSLQ PLIAIVYGPT GSGKSQLLRN
LLSCHLIDPS PETVFFVVPQ IDMIPPQEMS AWNAQLVEGN YTCGPQQTIV PKSGTLKPRL
ITLTYDDLTA DHNYDVTHPQ NIFAQAAQRG PICIIVDECM EELGKHKSIA KFFHAFPSKL
HDRFPQCTGY SVFVVLHNMN PRKDQAGNIA TLKIQSKCHI ISPKMQPSQV ARFINTYTKA
MPTAITLLLK DIFHHNANHI NYDWIIYNTS PEHECMQWMY LHPQNGLMPM YLNVQTILYQ
LLEKIDKVLR QRQRWNTAYS KKCDKLANK
//
