ID PSF2_DROME Reviewed; 203 AA.
AC Q9VQY9; M9PB28; Q8SYY9;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 139.
DE RecName: Full=Probable DNA replication complex GINS protein PSF2;
DE AltName: Full=GINS complex subunit 2;
GN Name=Psf2; ORFNames=CG18013;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: The GINS complex plays an essential role in the initiation of
CC DNA replication. {ECO:0000250}.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC Sld5, Psf1, Psf2 and Psf3. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9VQY9; Q9VBI1: Sld5; NbExp=3; IntAct=EBI-133591, EBI-124971;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GINS2/PSF2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL48856.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014134; AAF51021.3; -; Genomic_DNA.
DR EMBL; AE014134; AGB92558.1; -; Genomic_DNA.
DR EMBL; AY071234; AAL48856.1; ALT_INIT; mRNA.
DR RefSeq; NP_001260022.1; NM_001273093.1.
DR RefSeq; NP_608826.4; NM_134982.3.
DR PDB; 6RAW; EM; 3.70 A; L=1-203.
DR PDB; 6RAX; EM; 3.99 A; L=1-203.
DR PDB; 6RAY; EM; 4.28 A; L=1-203.
DR PDB; 6RAZ; EM; 4.46 A; L=1-203.
DR PDBsum; 6RAW; -.
DR PDBsum; 6RAX; -.
DR PDBsum; 6RAY; -.
DR PDBsum; 6RAZ; -.
DR AlphaFoldDB; Q9VQY9; -.
DR EMDB; EMD-2772; -.
DR EMDB; EMD-3318; -.
DR EMDB; EMD-3319; -.
DR EMDB; EMD-3320; -.
DR EMDB; EMD-3321; -.
DR EMDB; EMD-4785; -.
DR EMDB; EMD-4786; -.
DR EMDB; EMD-4787; -.
DR EMDB; EMD-4788; -.
DR SMR; Q9VQY9; -.
DR BioGRID; 59835; 12.
DR ComplexPortal; CPX-2670; GINS complex.
DR DIP; DIP-61215N; -.
DR IntAct; Q9VQY9; 10.
DR STRING; 7227.FBpp0303866; -.
DR PaxDb; 7227-FBpp0303867; -.
DR DNASU; 33641; -.
DR EnsemblMetazoa; FBtr0331449; FBpp0303866; FBgn0261976.
DR EnsemblMetazoa; FBtr0331450; FBpp0303867; FBgn0261976.
DR GeneID; 33641; -.
DR KEGG; dme:Dmel_CG18013; -.
DR UCSC; CG18013-RA; d. melanogaster.
DR AGR; FB:FBgn0261976; -.
DR CTD; 33641; -.
DR FlyBase; FBgn0261976; Psf2.
DR VEuPathDB; VectorBase:FBgn0261976; -.
DR eggNOG; KOG4071; Eukaryota.
DR GeneTree; ENSGT00390000007838; -.
DR HOGENOM; CLU_078274_2_0_1; -.
DR InParanoid; Q9VQY9; -.
DR OMA; DSLNCMY; -.
DR OrthoDB; 5487178at2759; -.
DR PhylomeDB; Q9VQY9; -.
DR Reactome; R-DME-176974; Unwinding of DNA.
DR BioGRID-ORCS; 33641; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 33641; -.
DR PRO; PR:Q9VQY9; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0261976; Expressed in capitellum (Drosophila) and 21 other cell types or tissues.
DR Genevisible; Q9VQY9; DM.
DR GO; GO:0071162; C:CMG complex; IDA:FlyBase.
DR GO; GO:0000811; C:GINS complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030261; P:chromosome condensation; IMP:FlyBase.
DR GO; GO:0006270; P:DNA replication initiation; IC:FlyBase.
DR GO; GO:0006271; P:DNA strand elongation involved in DNA replication; ISS:UniProtKB.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; ISS:UniProtKB.
DR CDD; cd11712; GINS_A_psf2; 1.
DR CDD; cd21694; GINS_B_Psf2; 1.
DR Gene3D; 1.20.58.1020; -; 1.
DR Gene3D; 3.40.5.50; -; 1.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR007257; GINS_Psf2.
DR PANTHER; PTHR12772; DNA REPLICATION COMPLEX GINS PROTEIN PSF2; 1.
DR PANTHER; PTHR12772:SF0; DNA REPLICATION COMPLEX GINS PROTEIN PSF2; 1.
DR Pfam; PF05916; Sld5; 1.
DR PIRSF; PIRSF028998; GINS_Psf2_subgr; 1.
DR SUPFAM; SSF158573; GINS helical bundle-like; 1.
DR SUPFAM; SSF160059; PriA/YqbF domain; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..203
FT /note="Probable DNA replication complex GINS protein PSF2"
FT /id="PRO_0000194816"
FT REGION 179..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 203 AA; 23114 MW; 632BEDDFB434B3DE CRC64;
MDPSIIEFIG EKCMISIIPN FSNEPLHLIY GPVGPFRAGF PVFVPLWMAT HLRKQQKCRI
VPPEWMDMDI LEEIKEEEKR SKFFTKMPCE HYMVVAQLVM STAPDDVPRC EELRTVIKDI
FDIRESKLRT SIDAFIKGEG TYAKLDNLTL LEIHSVRPIL PYSLDHIARY QRTATASQRD
TSMLSASMAG SSSGPNSNSL FSQ
//
