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Database: UniProt
Entry: Q00404
LinkDB: Q00404
Original site: Q00404 
ID   VHCA_METVO              Reviewed;         474 AA.
AC   Q00404;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   01-OCT-2014, entry version 53.
DE   RecName: Full=F420-non-reducing hydrogenase vhc subunit A;
DE            EC=1.12.99.-;
GN   Name=vhcA;
OS   Methanococcus voltae.
OC   Archaea; Euryarchaeota; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=2188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS;
RX   PubMed=1603063; DOI=10.1007/BF00587582;
RA   Halboth S., Klein A.;
RT   "Methanococcus voltae harbors four gene clusters potentially encoding
RT   two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-
RT   reducing or F420-non-reducing types.";
RL   Mol. Gen. Genet. 233:217-224(1992).
RN   [2]
RP   TRANSCRIPTIONAL REGULATION.
RC   STRAIN=ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS;
RX   PubMed=8121392; DOI=10.1007/BF00281785;
RA   Berghoefer Y., Agha-Amiri K., Klein A.;
RT   "Selenium is involved in the negative regulation of the expression of
RT   selenium-free [NiFe] hydrogenases in Methanococcus voltae.";
RL   Mol. Gen. Genet. 242:369-373(1994).
CC   -!- COFACTOR: Nickel. {ECO:0000305}.
CC   -!- SUBUNIT: The F420-non-reducing hydrogenase vhc is composed of
CC       three subunits; VhcA, VhcD and VhcG. {ECO:0000250}.
CC   -!- INDUCTION: By selenium deprivation. {ECO:0000269|PubMed:8121392}.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC       subunit family. {ECO:0000305}.
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DR   EMBL; X61203; CAA43506.1; -; Genomic_DNA.
DR   PIR; S16727; S16727.
DR   ProteinModelPortal; Q00404; -.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe_Hase-like.
DR   Pfam; PF00374; NiFeSe_Hases; 2.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   2: Evidence at transcript level;
KW   Metal-binding; Nickel; Oxidoreductase.
FT   CHAIN         1    474       F420-non-reducing hydrogenase vhc subunit
FT                                A.
FT                                /FTId=PRO_0000199729.
FT   METAL        61     61       Nickel. {ECO:0000255}.
FT   METAL        64     64       Nickel. {ECO:0000255}.
FT   METAL       445    445       Nickel. {ECO:0000255}.
FT   METAL       448    448       Nickel. {ECO:0000255}.
SQ   SEQUENCE   474 AA;  52914 MW;  4BA3F16EC5F18B44 CRC64;
     MTKLSIEPVT RVEGHGKVTL SFDDSGKLDK VNFHVVEVRG FEKFLEGRYI EDAPIFTPRI
     CGICQVSHHL ASAKAVDNVF GVKIPETANM LRNLIHQASN VHSHALHFGM LASPDLMFPT
     TDDALKRNLL GVAGENMDMI KDSIAMRKVG QTIVQKVGGR AIHPVTAIVG GQSKPLTEEE
     RDELLTLSDN LVETAERTLN VGKEMVENLK EKDLMNLGYF ESYHMGLVNN GAQDIYEGNI
     RVVNSEGKIE YEFDPAEYAN YITEGVRPYS YLKFLALLKR RGEGKGAYRV NTLSRLNVCD
     KMPTPIAQKY YEEFVKTYGK PAHQPMLFHY ARLIELLSSS ELIRKFLEDD KIVDTDVRAE
     VDPKNITGEG VGCVEAPRGT LIHHFKTDDR GIINDTNLVV ATVQNNPAMD IGVQKVAEKY
     IKTPEVAKPH ILNHMEMVIR AYDPCLSCAT HTIGEEPKIL SIHVCQGGKL IKTL
//
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