ID Q03KR2_STRTD Unreviewed; 280 AA.
AC Q03KR2;
DT 14-NOV-2006, integrated into UniProtKB/TrEMBL.
DT 14-NOV-2006, sequence version 1.
DT 29-MAY-2013, entry version 50.
DE RecName: Full=tRNA pseudouridine synthase B;
DE EC=5.4.99.25;
DE AltName: Full=tRNA pseudouridine(55) synthase;
DE AltName: Full=tRNA pseudouridylate synthase;
DE AltName: Full=tRNA-uridine isomerase;
GN Name=truB; OrderedLocusNames=STER_1000;
OS Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=322159;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-491 / LMD-9;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K., Slesarev A., Wolf Y., Sorokin A., Mirkin B., Koonin E.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.H., Diaz-Muniz I., Dosti B., Smeianov V.,
RA Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R.,
RA Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F.,
RA Broadbent J., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.,
RA Klaenhammer T., Richardson P., Kozyavkin S., Weimer B., Mills D.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC 55 in the psi GC loop of transfer RNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC Type 1 subfamily.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; CP000419; ABJ66210.1; -; Genomic_DNA.
DR RefSeq; YP_820406.1; NC_008532.1.
DR ProteinModelPortal; Q03KR2; -.
DR STRING; 322159.STER_1000; -.
DR EnsemblBacteria; ABJ66210; ABJ66210; STER_1000.
DR GeneID; 4438457; -.
DR KEGG; ste:STER_1000; -.
DR PATRIC; 19799320; VBIStrThe129203_1012.
DR eggNOG; COG0130; -.
DR HOGENOM; HOG000231225; -.
DR KO; K03177; -.
DR OMA; LGCGAYV; -.
DR ProtClustDB; PRK02484; -.
DR BioCyc; STHE322159:GJ9F-1000-MONOMER; -.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR HAMAP; MF_01080; TruB_bact; 1; -.
DR InterPro; IPR002501; PsdUridine_synth.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR015225; tRNA_psdUridine_synth_fam2_C.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF09142; TruB_C; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; Lyase; tRNA processing.
FT ACT_SITE 26 26 Nucleophile (By similarity).
SQ SEQUENCE 280 AA; 30525 MW; 293F640252FEDAEA CRC64;
MTSHDAVFKL RKILHEKKIG HGGTLDPDVT GVLPIAVGKA TRVIEYMTEA GKVYEGEITI
GFSTTTEDAS GEVVQTTPIT ELDGATVDQA MASFEGEITQ IPPMYSAVKI NGKKLYEYAR
AGEEVERPQR QVKITEFVRT SPIELENGTA RFTFRVACSK GTYVRTLSVD LGVKLGFASH
MSALRRTASA GLTLDSSLTL SQISEMVEAG DQSFLLPIEF GVQDLPAVQV TEDDAKEISF
GRFISINSQE PLVAAFLGDK VLAIMEKRGQ VYKPRKVLSQ
//
