ID ASSY_LEUMM Reviewed; 408 AA.
AC Q03W70;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 01-MAY-2013, entry version 48.
DE RecName: Full=Argininosuccinate synthase;
DE EC=6.3.4.5;
DE AltName: Full=Citrulline--aspartate ligase;
GN Name=argG; OrderedLocusNames=LEUM_1459;
OS Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 /
OS NCDO 523).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Leuconostoc.
OX NCBI_TaxID=203120;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8293 / NCDO 523;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B.,
RA Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N.,
RA Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K.,
RA Goodstein D.M., Hawkins T., Plengvidhya V., Welker D., Hughes J.,
RA Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B.,
RA Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E.,
RA Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C.,
RA Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J.,
RA Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S.,
RA Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC diphosphate + N(omega)-(L-arginino)succinate.
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC 1 subfamily.
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DR EMBL; CP000414; ABJ62552.1; -; Genomic_DNA.
DR RefSeq; YP_818925.1; NC_008531.1.
DR ProteinModelPortal; Q03W70; -.
DR STRING; 203120.LEUM_1459; -.
DR EnsemblBacteria; ABJ62552; ABJ62552; LEUM_1459.
DR GeneID; 4423189; -.
DR KEGG; lme:LEUM_1459; -.
DR PATRIC; 22410901; VBILeuMes50664_1450.
DR eggNOG; COG0137; -.
DR HOGENOM; HOG000230093; -.
DR KO; K01940; -.
DR OMA; NDQFRFE; -.
DR ProtClustDB; PRK00509; -.
DR BioCyc; LMES203120:GJ8T-1459-MONOMER; -.
DR UniPathway; UPA00068; UER00113.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.90.1260.10; -; 1.
DR HAMAP; MF_00005; Arg_succ_synth_type1; 1; -.
DR InterPro; IPR001518; Arginosuc_synth.
DR InterPro; IPR018223; Arginosuc_synth_CS.
DR InterPro; IPR023434; Arginosuc_synth_type_1_subfam.
DR InterPro; IPR024074; AS_cat/multimer_dom_body.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR11587; PTHR11587; 1.
DR Pfam; PF00764; Arginosuc_synth; 1.
DR TIGRFAMs; TIGR00032; argG; 1.
DR PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR PROSITE; PS00565; ARGININOSUCCIN_SYN_2; FALSE_NEG.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT CHAIN 1 408 Argininosuccinate synthase.
FT /FTId=PRO_0000321312.
FT NP_BIND 8 16 ATP (By similarity).
FT BINDING 86 86 Citrulline (By similarity).
FT BINDING 116 116 ATP; via amide nitrogen (By similarity).
FT BINDING 118 118 Aspartate (By similarity).
FT BINDING 122 122 Aspartate (By similarity).
FT BINDING 122 122 Citrulline (By similarity).
FT BINDING 123 123 Aspartate (By similarity).
FT BINDING 126 126 Citrulline (By similarity).
FT BINDING 174 174 Citrulline (By similarity).
FT BINDING 259 259 Citrulline (By similarity).
FT BINDING 271 271 Citrulline (By similarity).
SQ SEQUENCE 408 AA; 44912 MW; 50269E18745D5E45 CRC64;
MTDTLVLAYS GGLDTSVAIP WLKDKGYDVI AVVLDVGQHG KNLNEIQAKA LKVGAKQSIV
IDAKAEFADK YVAPVIKANM MYEGEYPMVS ALSRPLIIKK LVDIAHENDA VAIAHGSTGH
GNDQVRFEAA IHALDPDMKI EAPIRDFQWS REEEIQYAQE HDVPVPIDLD SPYSIDENLW
GRANEAGILE NPWNQAPEDA FALTTAIEDA PDTPEFIDVT FEAGVPVALN GEVLSLEKLI
VAVNEIAGKH GIGRIDHIEN RLVGIKSREI YEAPAAAVLM TAHKDLEDLT LERDVAHFKP
IVEQQLANLV YEAKWVSPLF DSLMAFIDST QQNVNGVVKM KLFKGNATAV ARQSEHNSLY
DEDLATYTSS SSFDQASAVG FIKLWTLSNT VYEQVNHVHS QAKKNTVK
//
