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Database: UniProt
Entry: Q04845
LinkDB: Q04845
Original site: Q04845 
ID   MTC1_CITFR              Reviewed;         376 AA.
AC   Q04845;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   16-OCT-2013, entry version 65.
DE   RecName: Full=Modification methylase CfrBI;
DE            Short=M.CfrBI;
DE            EC=2.1.1.113;
DE   AltName: Full=N(4)- cytosine-specific methyltransferase CfrBI;
GN   Name=cfrBIM;
OS   Citrobacter freundii.
OG   Plasmid pZE8.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=4111;
RX   PubMed=8335262; DOI=10.1016/0378-1119(93)90698-3;
RA   Zakharova M.V., Kravetz A.N., Beletzkaja I.V., Repyk A.V.,
RA   Solonin A.S.;
RT   "Cloning and sequences of the genes encoding the CfrBI restriction-
RT   modification system from Citrobacter freundii.";
RL   Gene 129:77-81(1993).
CC   -!- FUNCTION: This methylase recognizes the double-stranded sequence
CC       CCWWGG, causes specific methylation on C-? on both strands, and
CC       protects the DNA from cleavage by the CfrBI endonuclease.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA cytosine = S-
CC       adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       N(4) subfamily.
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DR   EMBL; X57945; CAA41012.1; -; Genomic_DNA.
DR   PIR; JN0745; JN0745.
DR   ProteinModelPortal; Q04845; -.
DR   REBASE; 3548; M.CfrBI.
DR   BRENDA; 2.1.1.113; 1400.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR017985; MeTrfase_CN4_CS.
DR   InterPro; IPR001091; RM_Methylase.
DR   Pfam; PF01555; N6_N4_Mtase; 1.
DR   PRINTS; PR00508; S21N4MTFRASE.
DR   PROSITE; PS00093; N4_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Plasmid; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    376       Modification methylase CfrBI.
FT                                /FTId=PRO_0000087926.
SQ   SEQUENCE   376 AA;  43358 MW;  2EE9E08E36616A21 CRC64;
     MTVKNNYLNR GYLVTSTTQA KKIAKLHLEK LDLADKFSFG LPEVDDRYHV WRVPLVATGH
     RIGEIVINSK TSTIDYKKSS KSPFLIKKAM EVVHTPKKVK KEKKIISKSP LNNMLLQGNC
     AETLKKLPDE SVNLVFTSPP YYNAKPEYSE YHTYDEYLSL LRSVIKECHR VLSEGRFFVI
     NVSPVLIRRA SRNEASKRIA VPFDLHRLFI EEGYEFIDDI HWVKPEGAGW ALGRGRRFAA
     DRNPLQYKPV PVTEYILVYR KKTDKLIDWN IRNHHSKEDV FDSKIGDDYE KTNLWKINPS
     RNRKHPATFP YGLAERVIKY YSFKNDVILD PFAGSGTTAK AAIDLGRRFV MCEISKQYID
     LIIEGTMLGG DLKIIN
//
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