ID MIAB_NITEC Reviewed; 443 AA.
AC Q0AF59;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 01-MAY-2013, entry version 51.
DE RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
DE EC=2.-.-.-;
DE AltName: Full=tRNA-i(6)A37 methylthiotransferase;
GN Name=miaB; OrderedLocusNames=Neut_1789;
OS Nitrosomonas eutropha (strain C91).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=335283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C91;
RX PubMed=17991028; DOI=10.1111/j.1462-2920.2007.01409.x;
RA Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S.,
RA Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M.,
RA Wei X.;
RT "Whole-genome analysis of the ammonia-oxidizing bacterium,
RT Nitrosomonas eutropha C91: implications for niche adaptation.";
RL Environ. Microbiol. 9:2993-3007(2007).
CC -!- FUNCTION: Catalyzes the methylthiolation of N6-
CC (dimethylallyl)adenosine (i(6)A), leading to the formation of 2-
CC methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37
CC in tRNAs that read codons beginning with uridine (By similarity).
CC -!- COFACTOR: Binds 2 4Fe-4S clusters. One cluster is coordinated with
CC 3 cysteines and an exchangeable S-adenosyl-L-methionine (By
CC similarity).
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methylthiotransferase family. MiaB
CC subfamily.
CC -!- SIMILARITY: Contains 1 MTTase N-terminal domain.
CC -!- SIMILARITY: Contains 1 TRAM domain.
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DR EMBL; CP000450; ABI60023.1; -; Genomic_DNA.
DR RefSeq; YP_747988.1; NC_008344.1.
DR ProteinModelPortal; Q0AF59; -.
DR STRING; 335283.Neut_1789; -.
DR EnsemblBacteria; ABI60023; ABI60023; Neut_1789.
DR GeneID; 4272267; -.
DR KEGG; net:Neut_1789; -.
DR PATRIC; 22721062; VBINitEut7577_2181.
DR eggNOG; COG0621; -.
DR HOGENOM; HOG000224767; -.
DR KO; K06168; -.
DR OMA; GIDRIRY; -.
DR ProtClustDB; PRK14325; -.
DR BioCyc; NEUT335283:GHT6-1834-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:HAMAP.
DR GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR GO; GO:0016740; F:transferase activity; IEA:HAMAP.
DR GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR Gene3D; 3.80.30.20; -; 1.
DR HAMAP; MF_01864; tRNA_metthiotr_MiaB; 1; -.
DR InterPro; IPR006638; Elp3/MiaB/NifB.
DR InterPro; IPR023970; MeThioTfrase/rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR006463; MiaB_methiolase.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR002792; TRAM_dom.
DR PANTHER; PTHR11918; PTHR11918; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SMART; SM00729; Elp3; 1.
DR TIGRFAMs; TIGR01574; miaB-methiolase; 1.
DR TIGRFAMs; TIGR00089; TIGR00089; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS50926; TRAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;
KW Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing.
FT CHAIN 1 443 (Dimethylallyl)adenosine tRNA
FT methylthiotransferase MiaB.
FT /FTId=PRO_0000374412.
FT DOMAIN 3 120 MTTase N-terminal.
FT DOMAIN 378 441 TRAM.
FT METAL 12 12 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 49 49 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 83 83 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 157 157 Iron-sulfur (4Fe-4S-S-AdoMet) (By
FT similarity).
FT METAL 161 161 Iron-sulfur (4Fe-4S-S-AdoMet) (By
FT similarity).
FT METAL 164 164 Iron-sulfur (4Fe-4S-S-AdoMet) (By
FT similarity).
SQ SEQUENCE 443 AA; 49757 MW; B24AEB28A94A5EEB CRC64;
MSSKLYIKTF GCQMNEYDSA KMADILLSEK NMELTEVPEE ADLILFNTCS VREKAQEKVF
HDLGRVRHLK NSKPDLLIGV GGCVASQEGS EIVRRAPFVD LVFGPQTLHR LPELIDARRR
TGQSQVDITF PEIEKFDRLP PARTTGATAF VSIMEGCSKY CSFCVVPYTR GEEVSRPLDD
VLTEVAGLVI QGVKEVTLLG QNVNAYYDKT SGEGDIDFAT LLDYIHEIPG LVRIRYTTSH
PREFTARLIE TYQRLPKLVG HVHLPIQSGS DRILAAMKRG YTIIEYKSII RKLRTIRPNI
SISSDFIVGF PGETDTDFEE TMKLIDDVKF DESFSFIYSP RPGTPASDLP DDTPYRIKLA
RLHQLQEKIQ RNAQMISQSM VDTIQRVLVE GPSKKDPNEF CGRTDNNRVV NFAGHAGLVG
SFVDIKITAV SSHTLRGEIS DMQ
//
