ID Q0B634_BURCM Unreviewed; 865 AA.
AC Q0B634;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN OrderedLocusNames=Bamb_4840 {ECO:0000313|EMBL:ABI90389.1};
OS Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia
OS (strain AMMD)).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=339670 {ECO:0000313|EMBL:ABI90389.1, ECO:0000313|Proteomes:UP000000662};
RN [1] {ECO:0000313|Proteomes:UP000000662}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-244 / AMMD {ECO:0000313|Proteomes:UP000000662};
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P.,
RA Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K.,
RA Ramette A., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome 2 of Burkholderia cepacia AMMD.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; CP000441; ABI90389.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0B634; -.
DR KEGG; bam:Bamb_4840; -.
DR eggNOG; COG1048; Bacteria.
DR Proteomes; UP000000662; Chromosome 2.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Lyase {ECO:0000313|EMBL:ABI90389.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 66..534
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 658..789
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 865 AA; 94086 MW; A5BB8AD9789176A5 CRC64;
MMNTAYRKPL PGTSLDYFDA RAAVEAIAPG AYDTLPYTSR VLAENLVRRC DPAILADSLK
QIIERKRDRD FPWFPARVVC HDILGQTALV DLAGLRDAIA DGGGDPAKVN PVVPVQLIVD
HSLAVECGGF DPDAFAKNRA IEDRRNEDRF HFIEWTKKAF ENVDVIPPGN GIMHQINLEK
MSPVIQAQDG VAFPDTCVGT DSHTPHVDAL GVIAIGVGGL EAENVMLGRA SWMRLPDIVG
VELTGKRQPG ITATDVVLAL TEFLRKEKVV GAYLEFRGEG AASLTLGDRA TISNMAPEYG
ATAAMFFIDG QTTDYLRLTG RSDEQVKLVE TYAKTAGLWA DTLGNVQYER TLTFDLSSVV
RNMAGPSNPH KRLPTSDLAA RGIAGQWEEK PGEMPDGAVI IAAITSCTNT SNPRNVIAAA
LLARNANAKG LTRKPWVKSS LAPGSKAVEL YLEEANLLPE LEKLGFGIVA FACTTCNGMS
GALDPKIQQE IVDRDLYATA VLSGNRNFDG RIHPYAKQAF LASPPLVVAY AIAGTIRFDI
EKDVLGHDQD GKPVTLKDIW PTDEEIDAIV ASSVKPEQFR KVYEPMFART ADAGERAAPL
YDWRAQSTYI RRPPYWEGAL AGERTLKGMR PLAVLGDNIT TDHLSPSNAI LLDSAAGEYL
AKMGLPEEDF NSYATHRGDH LTAQRATFAN PTLINEMAVV DGAVKKGSLA RVEPEGKVMR
MWEAIETYMN RKQPLIVIAG ADYGQGSSRD WAAKGVRLAG VEAIVAEGFE RIHRTNLIGM
GVLPLEFKPG TNRTTLGIDG TETFDVIGKR TPRADLTLVI QRKNGERVEV PVTCRLDTAE
EVSIYDAGGV LQRFAQDFLE SSQAA
//
