ID Q0BQ32_GRABC Unreviewed; 699 AA.
AC Q0BQ32;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 102.
DE SubName: Full=Isoamylase {ECO:0000313|EMBL:ABI63070.1};
DE EC=3.2.1.68 {ECO:0000313|EMBL:ABI63070.1};
GN OrderedLocusNames=GbCGDNIH1_2172 {ECO:0000313|EMBL:ABI63070.1};
OS Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Granulibacter.
OX NCBI_TaxID=391165 {ECO:0000313|EMBL:ABI63070.1, ECO:0000313|Proteomes:UP000001963};
RN [1] {ECO:0000313|EMBL:ABI63070.1, ECO:0000313|Proteomes:UP000001963}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1260 / CGDNIH1 {ECO:0000313|Proteomes:UP000001963};
RX PubMed=17827295; DOI=10.1128/JB.00793-07;
RA Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E.,
RA Kupko J.J.III., Barbian K.D., Babar A., Dorward D.W., Holland S.M.;
RT "Genome sequence analysis of the emerging human pathogenic acetic acid
RT bacterium Granulibacter bethesdensis.";
RL J. Bacteriol. 189:8727-8736(2007).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; CP000394; ABI63070.1; -; Genomic_DNA.
DR RefSeq; WP_011632872.1; NC_008343.2.
DR AlphaFoldDB; Q0BQ32; -.
DR STRING; 391165.GbCGDNIH1_2172; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GeneID; 69746354; -.
DR KEGG; gbe:GbCGDNIH1_2172; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_011725_1_1_5; -.
DR OrthoDB; 3236218at2; -.
DR Proteomes; UP000001963; Chromosome.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF9; GLYCOSYL HYDROLASE-RELATED; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000313|EMBL:ABI63070.1};
KW Hydrolase {ECO:0000313|EMBL:ABI63070.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001963}.
FT DOMAIN 140..569
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 466..486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..482
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 699 AA; 78968 MW; 23A7884A981E2E23 CRC64;
MRTRISEGLP YPLGATWDGL GVNFALFSAN ATKVELCLFD SSGMRELQRI ELPEYTDEVW
HGYLPDARPG QVYAYRVHGP YAPEVGHRFN PHKLLLDPYA KAMVGDLHWD DACHGYIIGD
EKEDLSFDTR DSARFMPKCR VIDSAFTWGR DHPPSVPWEK TVFYETHLRG YTMRHPMVPE
PLRGTFAGMS VKEVVKHIRS LGVTSIEFLP VHYFINDRFL IEQNLSNYWG YNTISFFAPH
PRYSATGAVA EFKEMVAHLH DAGLEVILDV VYNHTAEGNE RGPTLSFRGI DNASYYRLAP
DPRYYINDTG TGNTLNLSHP RVLQMVTDSL RYWVEEMHVD GFRFDLATIL AREAYGFDEG
GGFLDSCRQD PVLSRVKLVA EPWDLGPGGY QVGGFPPGWA EWNDRFRDTV RGFWKGEETA
SAMAARLCAS ADIYNRRGRK PWASVNFVTA HDGFTMQDVV TYNDKHNEAN GEDNKDGHSH
NRSWNCGVEG PSDDPALCAL RERQKRNLMA TLFLAKGTPM LLAGDEFGRT QQGNNNAYCQ
DNEISWVDWD IDADGHTLIA FVRTLISLRE RFPILRRGRF LTGAYDEELG VQDVTWIAAS
GDTMEPADWE NGTRCFGMLM DGRAQASGVR RAGTDTTLLV IYNAHHEEVP FHLPPCAGAR
RWELTFDTSQ PEGAKTRAYR IGQVYAVTAR SVVLFALQP
//
