ID ASSY_GRABC Reviewed; 416 AA.
AC Q0BVJ1;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 01-MAY-2013, entry version 48.
DE RecName: Full=Argininosuccinate synthase;
DE EC=6.3.4.5;
DE AltName: Full=Citrulline--aspartate ligase;
GN Name=argG; OrderedLocusNames=GbCGDNIH1_0263;
OS Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Granulibacter.
OX NCBI_TaxID=391165;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1260 / CGDNIH1;
RX PubMed=17827295; DOI=10.1128/JB.00793-07;
RA Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K.,
RA Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W.,
RA Holland S.M.;
RT "Genome sequence analysis of the emerging human pathogenic acetic acid
RT bacterium Granulibacter bethesdensis.";
RL J. Bacteriol. 189:8727-8736(2007).
CC -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC diphosphate + N(omega)-(L-arginino)succinate.
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC 1 subfamily.
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DR EMBL; CP000394; ABI61161.1; -; Genomic_DNA.
DR RefSeq; YP_744084.1; NC_008343.1.
DR ProteinModelPortal; Q0BVJ1; -.
DR SMR; Q0BVJ1; 15-411.
DR STRING; 391165.GbCGDNIH1_0263; -.
DR EnsemblBacteria; ABI61161; ABI61161; GbCGDNIH1_0263.
DR GeneID; 4274482; -.
DR KEGG; gbe:GbCGDNIH1_0263; -.
DR PATRIC; 22076553; VBIGraBet83793_0274.
DR eggNOG; COG0137; -.
DR HOGENOM; HOG000230093; -.
DR KO; K01940; -.
DR OMA; IYNGYWW; -.
DR ProtClustDB; PRK00509; -.
DR BioCyc; GBET391165:GHON-324-MONOMER; -.
DR UniPathway; UPA00068; UER00113.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.90.1260.10; -; 1.
DR HAMAP; MF_00005; Arg_succ_synth_type1; 1; -.
DR InterPro; IPR001518; Arginosuc_synth.
DR InterPro; IPR018223; Arginosuc_synth_CS.
DR InterPro; IPR023434; Arginosuc_synth_type_1_subfam.
DR InterPro; IPR024074; AS_cat/multimer_dom_body.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR11587; PTHR11587; 1.
DR Pfam; PF00764; Arginosuc_synth; 1.
DR TIGRFAMs; TIGR00032; argG; 1.
DR PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT CHAIN 1 416 Argininosuccinate synthase.
FT /FTId=PRO_0000263930.
FT NP_BIND 19 27 ATP (By similarity).
FT BINDING 46 46 ATP; via amide nitrogen and carbonyl
FT oxygen (By similarity).
FT BINDING 97 97 Citrulline (By similarity).
FT BINDING 102 102 Citrulline (By similarity).
FT BINDING 127 127 ATP; via amide nitrogen (By similarity).
FT BINDING 129 129 Aspartate (By similarity).
FT BINDING 133 133 Aspartate (By similarity).
FT BINDING 133 133 Citrulline (By similarity).
FT BINDING 134 134 Aspartate (By similarity).
FT BINDING 137 137 Citrulline (By similarity).
FT BINDING 188 188 Citrulline (By similarity).
FT BINDING 197 197 Citrulline (By similarity).
FT BINDING 273 273 Citrulline (By similarity).
FT BINDING 285 285 Citrulline (By similarity).
SQ SEQUENCE 416 AA; 46235 MW; 23AAA2BA61B153C6 CRC64;
MTRDIQTMAV KDVKKVVLAY SGGLDTSVIL RWLQTTYNCE VVTFTADLGQ GEELEPARRK
AEMFGVKEIF VDDLRETFVK DFVFPMFRAN AVYEGQYLLG TSIARPLIAQ RQIEIAELTG
ADAVAHGATG KGNDQVRFEL SYYALKPDVK VIAPWREWDL TSRTKLLEFA EANQIPIAKD
KRGEAPFSVD ANLLHSSSEG KLLEDPAEEP DEIVYQRTIS PEAAPDKATI ITIDFEHGDP
VAIDGIRLSP ATLLAKLNEL GKANGIGRLD LVENRFVGMK SRGVYETPGG TILLVAHRGI
ESITLDREAA HLKDSLMPRY AELIYNGFWF SPERRMLQAA IDESQKSVTG RVRLKLYKGN
TIVIGRESPN SLYSLKHVTF EDDQGAYDQV DAQGFIKLQS LRLRLAAIAG RRGGSL
//
