ID Q0IGE7_AEDAE Unreviewed; 1226 AA.
AC Q0IGE7;
DT 03-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2006, sequence version 1.
DT 24-JAN-2024, entry version 120.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=AAEL000388 {ECO:0000313|EMBL:EAT48591.1};
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159 {ECO:0000313|EMBL:EAT48591.1, ECO:0000313|Proteomes:UP000682892};
RN [1] {ECO:0000313|EMBL:EAT48591.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Liverpool {ECO:0000313|EMBL:EAT48591.1};
RA Loftus B.J., Nene V.M., Hannick L.I., Bidwell S., Haas B., Amedeo P.,
RA Orvis J., Wortman J.R., White O.R., Salzberg S., Shumway M., Koo H.,
RA Zhao Y., Holmes M., Miller J., Schatz M., Pop M., Pai G., Utterback T.,
RA Rogers Y.-H., Kravitz S., Fraser C.M.;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EAT48591.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Liverpool {ECO:0000313|EMBL:EAT48591.1};
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
RN [3] {ECO:0000313|EMBL:EAT48591.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Liverpool {ECO:0000313|EMBL:EAT48591.1};
RG VectorBase;
RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; CH477192; EAT48591.1; -; Genomic_DNA.
DR RefSeq; XP_001656071.1; XM_001656021.1.
DR AlphaFoldDB; Q0IGE7; -.
DR STRING; 7159.Q0IGE7; -.
DR PaxDb; 7159-AAEL000388-PA; -.
DR GeneID; 5576333; -.
DR KEGG; aag:5576333; -.
DR VEuPathDB; VectorBase:AAEL000388; -.
DR eggNOG; KOG0434; Eukaryota.
DR HOGENOM; CLU_001493_1_1_1; -.
DR OMA; EIIVIHK; -.
DR OrthoDB; 656at2759; -.
DR PhylomeDB; Q0IGE7; -.
DR Proteomes; UP000682892; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035}.
FT DOMAIN 25..647
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 704..855
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1226 AA; 141963 MW; 9BF35149E956C336 CRC64;
MDSVTVEDLR RLPESISFPN EEEKVFAYWK QEKVFEACLK QSKGKPRYTF YDGPPFATGL
PHYGHILAGT VKDIVTRYAH QQGYHVERRF GWDCHGLPVE YEIDKTLNIR GPEDVAKMGI
KAYNNECRKI VMRYADEWEQ IIGRMGRWID FKNDYKTLYP WYMESIWWVF KQLYVKGMVY
QGVKVMPYST ACTTALSNFE SGQNYKEVTD PAVFVSFPIV GDKDNAALIG WTTTPWTLPS
NMACCVHPEL IYARVKELKT GKVYIMMECR IESLFKGPEN YEILDRFPGN KLAGVKYEPL
FDYFSKYDKV GFRVLVDTYV TEESGTGVVH QAPYFGEDDY RVCLANGVIT RDQEIVCPVD
PSGKFMDPVS DFKGQYVKDA DKHIIKLLKE RGRLVFSSQV KHNYPFCWRS DTPLIYKAVP
SWFVRVEHMT KQLLSCSSQT YWVPEYVKEK RFGNWLRDAR DWAISRNRYW GTPIPLWISP
DGQEIVCIGS IEELEKYSGV RVNDLHRESI DHIEIPSSVP GNPPLRRVPE VFDCWFESGS
MPFAQNHYPF ENASDFLSNS FPADFIAEGI DQTRGWFYTL LVISTALFNK PPFKNLNCTG
LVLASDGQKM SKRKKNYPDP MEVVHKYGAD ALRLYLINSP VVRAENLRFK EEGVKDIVKD
VFLPWYNAFR FLLQNIDRFE KEEKLVYRYD AVRHAKNRST NVMDVWITSF KESLLDFVAK
EMKAYHLYTV VPRLTKFIDQ LTNWYVRMNR KRIKGEFGVE DCYHALDTLY DVLLAMVKMM
APFTPYLTEY MFQRLRLLNT EKIDGSVHFQ MMPGSNKKYI NEPIERAVSR MQAVVELGRV
MRDRRTVPIK YPLTEVIVIH QSKEYLDDIK SLENFILDEL NVRKITLSSD KQKYGVKLRA
EPDHKVLGIR LKNDFKQVIQ AVKALTDEQI NEQVKIGHFT VLGHRIELNE LRLIYQFDEK
QTEGHNYEAH SDNDVLVLLD MTPNEELMKE GVAREIINRI QKLKKKAKLI PTDPVLIYYT
VSKDGEIKSV AESHQEFIVN IVKSPFLPYG PEAATKQVLI EESQELKGVQ LNLVICSPKD
RPIPASPWVN LVLDEKIAPR YQAKPSREAT VLLTNIKGDL LTLNQLNVEI HSLFGLNDQS
YKLLTADGKQ LDQVDLKLNQ QTVFITHGTS LPNGWKPAKA PSCAFRNVEY KGRKATLLEE
NPVGVKLNVE QVMKRFFPDA KQLKVN
//
