ID   Q0N8M7_VARV             Unreviewed;      1286 AA.
AC   Q0N8M7;
DT   19-SEP-2006, integrated into UniProtKB/TrEMBL.
DT   19-SEP-2006, sequence version 1.
DT   27-MAR-2024, entry version 54.
DE   RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide {ECO:0000256|ARBA:ARBA00021673};
DE            EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
GN   ORFNames=VARV_SUM70_222_087 {ECO:0000313|EMBL:ABG45481.1},
GN   VARV_SUM70_228_087 {ECO:0000313|EMBL:ABF28072.1};
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255 {ECO:0000313|EMBL:ABF28072.1, ECO:0000313|Proteomes:UP000122860};
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1] {ECO:0000313|Proteomes:UP000117241, ECO:0000313|Proteomes:UP000122860}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sumatra 1970 V70-222 {ECO:0000313|EMBL:ABG45481.1}, and Sumatra
RC   1970 V70-228 {ECO:0000313|EMBL:ABF28072.1};
RX   PubMed=16873609; DOI=10.1126/science.1125134;
RA   Esposito J.J., Sammons S.A., Frace A.M., Osborne J.D., Olsen-Rasmussen M.,
RA   Zhang M., Govil D., Damon I.K., Kline R., Laker M., Li Y., Smith G.L.,
RA   Meyer H., LeDuc J.W., Wohlhueter R.M.;
RT   "Genome sequence diversity and clues to the evolution of variola (smallpox)
RT   virus.";
RL   Science 313:807-812(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 147
CC       kDa subunit family. {ECO:0000256|ARBA:ARBA00006884}.
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DR   EMBL; DQ441442; ABF28072.1; -; Genomic_DNA.
DR   EMBL; DQ437591; ABG45481.1; -; Genomic_DNA.
DR   Proteomes; UP000117241; Genome.
DR   Proteomes; UP000122860; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 2.40.40.20; -; 1.
DR   Gene3D; 6.10.250.2940; -; 1.
DR   Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR   Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR   PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR   SMART; SM00663; RPOLA_N; 1.
DR   SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          188..469
FT                   /note="RNA polymerase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00663"
SQ   SEQUENCE   1286 AA;  146785 MW;  50FE9F1D0B33C54C CRC64;
     MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF
     GHWGKVSIYK THIVKPEFIS EIIRLLNHIC IHCGLLRSRE PYSNDINLKE LSGHALRRLK
     DKILSKKKSC WNSECMQPYQ KISFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL
     EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI
     QKAVIEYDDI KIISNNTTSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE
     VGMPAYIRNT LTEKIFVNAF TVNKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL
     LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIAN SQNADFDGDE
     EWMILEQNPK AVVEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL
     NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS
     LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE
     LIKEAYAKYL KDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDNPDNN
     LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN
     SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL
     IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF
     NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI
     TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG
     FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPNITLR KETDRYVVDI
     IVNRLYIKRV EITELVVEYM IERFISFSVI VKEWGMETFI EDEDNIRFTV YLNFVEPEEL
     NLSKFMMVLP GAANKGKISK FKIPISDYTG YDDFNQTKKL NKMTVELMNL KELGSFDLEN
     VNVYPGVWNT YDIFGIEAAR GYLCEAMLNT YGEGFDYLYQ PCDLLASLLC ASYEPESVNK
     FKFGAASTLK RATFGDNKAL LNAALHKKSE PINDNSSCHF FSKVPNIGTG YYKYFIDLGL
     LMRMERKLSD KISSQKIKEM EETEDF
//