ID GPDA_CLOP1 Reviewed; 331 AA.
AC Q0TPK0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 01-MAY-2013, entry version 59.
DE RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(P)+];
DE EC=1.1.1.94;
DE AltName: Full=NAD(P)H-dependent glycerol-3-phosphate dehydrogenase;
GN Name=gpsA; OrderedLocusNames=CPF_2007;
OS Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A).
OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=195103;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13124 / NCTC 8237 / Type A;
RX PubMed=16825665; DOI=10.1101/gr.5238106;
RA Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R.,
RA DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C.,
RA Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J.,
RA Sullivan S.A., Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S.,
RA Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H.,
RA Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I.,
RA Melville S.B., Paulsen I.T.;
RT "Skewed genomic variability in strains of the toxigenic bacterial
RT pathogen, Clostridium perfringens.";
RL Genome Res. 16:1031-1040(2006).
CC -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(P)(+) =
CC glycerone phosphate + NAD(P)H.
CC -!- PATHWAY: Membrane lipid metabolism; glycerophospholipid
CC metabolism.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC dehydrogenase family.
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DR EMBL; CP000246; ABG82368.1; -; Genomic_DNA.
DR RefSeq; YP_696440.1; NC_008261.1.
DR ProteinModelPortal; Q0TPK0; -.
DR STRING; 195103.CPF_2007; -.
DR EnsemblBacteria; ABG82368; ABG82368; CPF_2007.
DR GeneID; 4202431; -.
DR KEGG; cpf:CPF_2007; -.
DR PATRIC; 19486266; VBICloPer106549_1952.
DR eggNOG; COG0240; -.
DR HOGENOM; HOG000246854; -.
DR KO; K00057; -.
DR OMA; KHEMEEV; -.
DR ProtClustDB; PRK00094; -.
DR BioCyc; CPER195103:GHAW-2100-MONOMER; -.
DR UniPathway; UPA00940; -.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0047952; F:glycerol-3-phosphate dehydrogenase [NAD(P)+] activity; IEA:HAMAP.
DR GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0046167; P:glycerol-3-phosphate biosynthetic process; IEA:HAMAP.
DR GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR GO; GO:0006650; P:glycerophospholipid metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0008654; P:phospholipid biosynthetic process; IEA:HAMAP.
DR Gene3D; 1.10.1040.10; -; 1.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_00394; NAD_Glyc3P_dehydrog; 1; -.
DR InterPro; IPR008927; 6-PGluconate_DH_C-like.
DR InterPro; IPR013328; DH_multihelical.
DR InterPro; IPR006168; G3P_DH_NAD-dep.
DR InterPro; IPR006109; G3P_DH_NAD-dep_C.
DR InterPro; IPR011128; G3P_DH_NAD-dep_N.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR PANTHER; PTHR11728; PTHR11728; 1.
DR Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR PRINTS; PR00077; GPDHDRGNASE.
DR SUPFAM; SSF48179; 6DGDH_C_like; 1.
DR PROSITE; PS00957; NAD_G3PDH; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Lipid biosynthesis; Lipid metabolism;
KW NAD; Oxidoreductase; Phospholipid biosynthesis;
KW Phospholipid metabolism.
FT CHAIN 1 331 Glycerol-3-phosphate dehydrogenase
FT [NAD(P)+].
FT /FTId=PRO_1000049497.
FT NP_BIND 8 13 NAD (By similarity).
FT REGION 253 254 Substrate binding (By similarity).
FT ACT_SITE 189 189 Proton acceptor (By similarity).
FT BINDING 106 106 NAD; via amide nitrogen (By similarity).
FT BINDING 106 106 Substrate (By similarity).
FT BINDING 138 138 NAD; via amide nitrogen (By similarity).
FT BINDING 253 253 NAD (By similarity).
FT BINDING 279 279 NAD (By similarity).
SQ SEQUENCE 331 AA; 35649 MW; 2A372299389E91A2 CRC64;
MSKVAFLGAG SFGTSLGILL GNKGVTVSLW DRDENVINDI NVNRKNDKYI KDLTIPTNVT
AYKDLDEALN GAEYVVLAVP SHVIRTACKN LKGKINDDVI IINIAKGIEE GTNLRLSQVI
NQELPNNKVV VLSGPSHAEE VSKGIPTTLV ASSECMECAE KVQDLFMDKN FRIYTNDDII
GVEIGGAVKN IIALAAGVCD GIGYGDNSKA ALMTRGMAEI ARIGIKMGGK AETFFGLTGM
GDLIVTCTSM HSRNRRAGIL IGQGKTAEEA IEEVGMVVEG IKACKAFYEL KEKEGVTMPI
TDIAYKVLFE GAKAENAVSL LMERDKKKEE I
//
