ID Q0TY23_PHANO Unreviewed; 3933 AA.
AC Q0TY23;
DT 05-SEP-2006, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 2.
DT 24-JAN-2024, entry version 115.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=SNOG_15653 {ECO:0000313|EMBL:EAT77028.2};
OS Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume
OS blotch fungus) (Parastagonospora nodorum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Phaeosphaeriaceae;
OC Parastagonospora.
OX NCBI_TaxID=321614 {ECO:0000313|EMBL:EAT77028.2, ECO:0000313|Proteomes:UP000001055};
RN [1] {ECO:0000313|Proteomes:UP000001055}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SN15 / ATCC MYA-4574 / FGSC 10173
RC {ECO:0000313|Proteomes:UP000001055};
RX PubMed=18024570; DOI=10.1105/tpc.107.052829;
RA Hane J.K., Lowe R.G., Solomon P.S., Tan K.C., Schoch C.L., Spatafora J.W.,
RA Crous P.W., Kodira C., Birren B.W., Galagan J.E., Torriani S.F.,
RA McDonald B.A., Oliver R.P.;
RT "Dothideomycete-plant interactions illuminated by genome sequencing and EST
RT analysis of the wheat pathogen Stagonospora nodorum.";
RL Plant Cell 19:3347-3368(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; CH445363; EAT77028.2; -; Genomic_DNA.
DR RefSeq; XP_001805797.1; XM_001805745.1.
DR STRING; 321614.Q0TY23; -.
DR EnsemblFungi; SNOT_15653; SNOT_15653; SNOG_15653.
DR GeneID; 5982727; -.
DR KEGG; pno:SNOG_15653; -.
DR VEuPathDB; FungiDB:JI435_156530; -.
DR eggNOG; KOG0939; Eukaryota.
DR HOGENOM; CLU_000215_0_1_1; -.
DR InParanoid; Q0TY23; -.
DR OrthoDB; 164548at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000001055; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000001055};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 3597..3933
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 220..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 274..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 686..709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1105..1135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1448..1542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1882..1910
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1956..1994
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2254..2290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2303..2426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2545..2576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2712..2811
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2852..2886
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2907..2937
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3083..3103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3205..3301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1110..1126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1509..1534
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1962..1988
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2303..2329
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2345..2425
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2712..2761
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2765..2784
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2907..2923
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3205..3222
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3236..3277
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3278..3299
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3900
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3933 AA; 436226 MW; F19CA9FE7829F505 CRC64;
MKIKKQATSR HESTLSSMIA EFVRATESIP LYQLPAHLAS FPKHWPFPRG DAYHWIPVLN
RFDRILELFN QEYGLVDGPQ TQPFGRRLLL KGDAEEGSTS SDQTTTDAIL DTLHVSQDGD
RELVENVLNF TRMLLENCGN RSLYASSERL DKLLNSTSTP LLKATLRLGH RLAQRFSAAR
QRLGSANLHL SLLASHYNIN LDKVQKLSAP FSKGPAVSAP IFGTPSGKGK DKIADRRGDT
ERISPSNLVG LYALPDSTLK QEFGGVALSY YEPTPATEEA TSKPTATDPP TTPTPIRRTS
NMGPNRTPRQ PQSAPTTDSP TTPAFTPGNP SSKTTGPKTF ELSAEKVSSS DLHQLIKQGL
ADLPDSVHYE FLHKVRTAKL LAAGKTGRDD AIALRMLAIA NSGYVYGEKE FHAKVGQQDA
DEPRRLQLAY QLSELVHPPS NGERGISTEL QTFVLGALEA LAKHKGKGAD ICTALSVNVN
HGVLFYLVRK LVAGLGTETP AADYLEEDQW RDALFSLLNT LPVSQQRTGE GMVSAGLLEI
LVEALKLRTE KAEPLVNAKG LDIIADMMQY EVDIAKKLAE DNKGMPQEYK TTIDGTLRWL
FKFLNHMMGH TGTNNDRLMR TLIDSAQLLT GLRTVLSNAR IYGSTVWSTA VLILTSFIHN
EPTSYQVIAE ANLSAAFLES VAGEPAAESP EALEENNGHS DTVPNRTYAP RVPGEPLAAG
ILPVAEAIST LPPAFGAICL AESGMKLFQS STALKRFFEI FESPEHIKAL DADTEMPTMI
GNSFDELVRH HPPLKARVLS CLSELIARVV HLCAERAEKE GIGAKLWVET AEGQLIVAGG
RQALSGSQTH RRIVPQPDAT GNDIVMQDTD EEATTGSVIE AVTAEQVTET EDSTKGPTTG
QYIRVLCRFL SGFFSNHAMC SAYIEANGVE SILDISSLAC LDPRSNESRS MTEEFSRVVQ
VLVEQKPHIA VPSLIKRTQQ ALEHLEPMLD HTGAQAFFAP FTSAGTARDD GNLHNGTEYV
KSLVSAFTLV GAMTLTFQSQ MYNARGAHSL SSQVNLADIL GKLHRSCVWE ELSLQRNMPA
EWEKATRVKS TGFGNAEADL VFRMGTSDRP SETAAPSTTE DSSSGDAAAP EPSVAKTSVL
SQESAQFKNT QILRNLLSKI PTEIAPFCQS LGKLLLFRRS LEAYQRQCAT VVSDQLAQAV
IDQLQYRGPQ ESDSAEDRYA YWVVILTSLS QLMIDANLDR PQALTLLLVS FRNLGGFEVL
GDILGKFYQS ALEVTQKQGE DANEESQRLL NLSLGGIKII LAFYSQITSF KIIGESQQTS
TMQSRPDRDR ERADYFSTAQ FLVELRFAVI KPVEKIWNSD LMDKATTSIV KTSINILKSV
LDCDGEHGAY ATADKIPKRS KPIIKPWTPR NAGYIQQLKD ADFSESLAEE ALYRCCDNLN
MAREYCQNQA RPQSSRNPIP QYELEARGPP SSSPSRAEVV VPEPTDSVSM SSSDNTQDDE
GSEAPDTQSI EMEDVSSQDE TGEPSGTPVP DSRTDASGPT PHDALAYQKR MRAGNMAEAV
ALEDLDKART ELRQTLVDRS LDILNSHDDV TFELADLITA AVAKAADPGS MRNEIGTTLV
QSLISLQSED DFRSQGKKIS ALSASIGFDF YDVIVDELKD NFVTLLGFVK IFPDQPADES
SPWIGQVLLI IEKLLAEDQL PQQITWTPPT GDSTEPISAD QLPEPIVGVD EKNQLFDAIM
EMLPRISKDE SLALSVTRVL VMLTRTRKIA ARLAEKRNIQ RLFLMIKQLA GITNEGLRSA
LMIVLRHMIE DEAMIRQIMG TEIQQMFESR DRRQTDTTGY TRQMYAHAVR APELFVEVTN
EKLQLARFDP NQRPQTLILK REEAPSAEAD ALPGATTGPV DAPVEEDRPK ETIEPKPLLE
RTKTADLKVP MVENSDGVIH YLLCELLAYK EIDDTPEPAK TSEATPEVSG SEQAAPEASS
SAVTTTPELS KPEKPVFKAD THPIYIYRCF LLKCLAELLQ SYNRTKIEFI SFSRKADPYT
TTPSKPRSGV LNYLLNTLVP VGTLNHEGDL AFKKKLATSN CAIDVVVSLC HKTGEYTVPK
AEAQQSPYAE TEQDLLFVRK FVLEHALKAF RDANAWDEPL DMKYSRLLGI SDIFSRMISQ
RSNGEMLTQN AELTPNLKQM AKIMYEKNFI TILTTAISDI DLNFPNAKRV VKYILKPMKW
LTYVAMDLSM HYDTSSAPDS ADEYEISTAS DDDLVDTGRE ETPDLFRNST LGMFEMPHDS
ASEDSEDEDG EEIMYDDAYA DDMEYDEDEP GDRDEVVSDD DEDMMDPDLG DIGPIEGMPG
DIEIEVEMGG DEDGMGLDSD DNSEDDEGDD EEDDDDEDDM DDDDDDDDMD DDEAMEALEA
LEEVTGDDEN ASLGDDQEDD WSDDAGDFGI INGEGNLPPG ALDGIVLESG TRMIDERGHM
AGFLGEEMGE EDDEEGDEFD DEDIHYDVEA EDDDDDMADL GAWTGGWDEP APPAHRHGHR
LSPWMFPAGP GDRILVPAYR SHRPGAGPRV TDDGINPLLQ RGGRSSGRGT EGPFRNEGMS
DWVHAIENRG PRVFPSDSPV SFISNLLNAM SQGNGPVLHQ AGGALHLSFN SVPMGIPHPF
DSGLRREANR MREMSRSARE DPHSAITFVK ASTSQRWQEE ARLLYGPNAI EKGSRVVNSI
LKVMVPPAME AAKKRQEERE AEAARKAKEE QALKEQQERE EREAKEKEER ERQEREAAEA
ASRANEQTAE PQAADETASA TQAQAMEGVE AGATAPEEPA EPSEPAADTR PRIMTTLRGH
EYDITDLGID LEYLDALPEE LREEVLMQQV AERRQEERAQ RQQQQQAQPQ QAAAEPAAPS
GDQPTDIDEQ FLAALPPEIR EELLQQEAAE RRRREREENR RRNQTGGVAP QAEDMDTASF
LASLDPTLRA AVLMDTDEDT LRQLPPEISA EARAYGGDRR LNQFNEYGYR RGDRRGQQED
ATQKKKARPC VQMLDKAGVA TLLRLMFIPQ QGSAKTSLSS ILRYVCENRQ NRAEVISILL
SILQDGSADV NAVERSFAQL SIRAKQPQQP ADKTPKLSRK NGSLSINADV SPLMVVQQCL
NTLTQLCDKN PAVWQFFLTE HETGVGFKNR GNRKGKAKDT KDAKATRFPV NALLTLLDRK
LIVESSSIME QLTALLKHIT QPLHSLKSDK DKAKDEAEKQ TDSANIPGHA IDVAPNVEQS
ASTSEPQTGV QDTEMTATSE AAGQPTDTAT EGESSTTTAK DTDPKPEDDK SKKHRPLIPP
EIPENNLRLV AKILAARECN SKVFQETLSV ISNLSPIPGA KEIFGHELLG IAKDLARSTL
DDLASLTVQV SKADSPTDVQ GIALAKFSPA SSDQTKLLRA LTALDYLFDP SRDSKDKPEA
RAEALEPAQK EDILLSLYED AAFAPLWEKL SGCLTVIRQR GNMLNIATTL LPLIESLMVV
CKNTTLKDVP LAKMLPKEFA LSSPPPENKM ENLFFNFTEE HRKILNDLVR QNPKLMSGTF
SLLVKNSKVL EFDNKRNYFN RKLHSRSGDR QPHPPLQLAV RRDQVFLDSF KSLYFKSADE
MKYGKLSIRF HGEEGVDAGG VTREWFQVIS RQMFNADYAL FVPVASDRTT FHPNRLSSIN
PEHLMFFKFI GRIIGKALYE GRVLDCHFSR AVYKQIMGKQ VNLKDMETLD LEYYKSLEWM
LNNDITDIIT ETFSVEVEAF GEMQVVDLID NGREIPVTED NKHEYIRLIT EHRLTGSVRE
QLDHFLKGFH DIVPSTLVSI FSEQELELLI SGLPDINVDD WKNNTEYHNY TAASPQIQWF
WRAVRTFEKE EQAKLLQFVT GTSKVPLNGF KELEGMNGFS KFNIHRDFGS KDRLPSSHTC
FNQLDLPEYE TYEDLRKALY TAMTAGGEYF GFA
//
