ID Q0WMJ4_ARATH Unreviewed; 487 AA.
AC Q0WMJ4;
DT 05-SEP-2006, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2006, sequence version 1.
DT 27-MAR-2024, entry version 132.
DE RecName: Full=microtubule-severing ATPase {ECO:0000256|ARBA:ARBA00038871};
DE EC=5.6.1.1 {ECO:0000256|ARBA:ARBA00038871};
GN OrderedLocusNames=At2g45500 {ECO:0000313|Araport:AT2G45500,
GN ECO:0000313|EMBL:AEC10563.1};
GN ORFNames=F17K2.3 {ECO:0000313|EMBL:AEC10563.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000313|EMBL:BAF01657.1};
RN [1] {ECO:0000313|EMBL:AEC10563.1, ECO:0000313|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S., Shea T.P., Benito M.I., Town C.D.,
RA Fujii C.Y., Mason T., Bowman C.L., Barnstead M., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2] {ECO:0000313|EMBL:BAF01657.1}
RP NUCLEOTIDE SEQUENCE.
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Morosawa T.,
RA Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K.,
RA Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K.,
RA Akiyama K., Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA Hayashizaki Y., Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AEC10563.1}
RP NUCLEOTIDE SEQUENCE.
RG TAIR;
RA Swarbreck D., Lamesch P., Wilks C., Huala E.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AEC10563.1}
RP NUCLEOTIDE SEQUENCE.
RA Krishnakumar V., Cheng C.-Y., Chan A.P., Schobel S., Kim M., Ferlanti E.S.,
RA Belyaeva I., Rosen B.D., Micklem G., Miller J.R., Vaughn M., Town C.D.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|Proteomes:UP000006548}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)
CC alpha/beta tubulin heterodimers.; EC=5.6.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00036378};
CC -!- SIMILARITY: Belongs to the AAA ATPase family.
CC {ECO:0000256|RuleBase:RU003651}.
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DR EMBL; CP002685; AEC10563.1; -; Genomic_DNA.
DR EMBL; AK229827; BAF01657.1; -; mRNA.
DR RefSeq; NP_001078063.1; NM_001084594.2.
DR AlphaFoldDB; Q0WMJ4; -.
DR SMR; Q0WMJ4; -.
DR ProteomicsDB; 191722; -.
DR EnsemblPlants; AT2G45500.2; AT2G45500.2; AT2G45500.
DR GeneID; 819158; -.
DR Gramene; AT2G45500.2; AT2G45500.2; AT2G45500.
DR KEGG; ath:AT2G45500; -.
DR Araport; AT2G45500; -.
DR TAIR; AT2G45500; -.
DR HOGENOM; CLU_000688_21_12_1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q0WMJ4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IEA:UniProt.
DR CDD; cd02679; MIT_spastin; 1.
DR CDD; cd19524; RecA-like_spastin; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 1.20.58.80; Phosphotransferase system, lactose/cellobiose-type IIA subunit; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR007330; MIT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR23074; AAA DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR23074:SF86; SPASTIN; 1.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17862; AAA_lid_3; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00745; MIT; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|RuleBase:RU003651};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00023212};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Microtubule {ECO:0000256|ARBA:ARBA00022701};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU003651};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q0WMJ4,
KW ECO:0007829|ProteomicsDB:Q0WMJ4};
KW Reference proteome {ECO:0000313|Proteomes:UP000006548}.
FT DOMAIN 52..129
FT /note="MIT"
FT /evidence="ECO:0000259|SMART:SM00745"
FT DOMAIN 248..384
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 19..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..161
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 487 AA; 53944 MW; 4C79B7E240D2B15B CRC64;
MSFLRGIIDS FSSILNEESK KDPSVSSSST SSESMNGIDG VPVTNERIAY KLKGYFDLAK
EEIAKGVRAE EWGLHDDALL HYRNAQRIMN EATSTPSPSY ISSSEKEKVR SYREKISNWQ
NQVSERLQAL GVGMSENKRT VAYPSSASVS STASRYRKTL SQKTPVARGG VATPRNPKDA
AASPKPVKES GNVYDDKLVE MINTTIVDRS PSVKWDDVAG LNGAKQALLE MVILPAKRRD
LFTGLRRPAR GLLLFGPPGN GKTMLAKAVA SESQATFFNV SASSLTSKWV GEAEKLVKTL
FQVAISRQPS VIFMDEIDSI MSTRSTSENE ASRRLKSEFL IQFDGVTSNP DDLVIIIGAT
NKPQELDDAV LRRLVKRIYV PLPDSNVRKL LFKTKLKCQP HSLSDGDIDK IVKETEGYSG
SDLQALCEEA AMMPIRELGA NILTIQANKV RSLRYDDFRK SMAVIRPSLS KSKWEELERW
NSEFGSN
//
