ID Q0ZNW5_MALDO Unreviewed; 213 AA.
AC Q0ZNW5;
DT 22-AUG-2006, integrated into UniProtKB/TrEMBL.
DT 22-AUG-2006, sequence version 1.
DT 24-JAN-2024, entry version 64.
DE RecName: Full=glutathione dehydrogenase (ascorbate) {ECO:0000256|ARBA:ARBA00012436};
DE EC=1.8.5.1 {ECO:0000256|ARBA:ARBA00012436};
GN Name=DHAR {ECO:0000313|EMBL:ABG49123.1};
GN ORFNames=DVH24_023159 {ECO:0000313|EMBL:RXI09015.1};
OS Malus domestica (Apple) (Pyrus malus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX NCBI_TaxID=3750 {ECO:0000313|EMBL:ABG49123.1};
RN [1] {ECO:0000313|EMBL:ABG49123.1}
RP NUCLEOTIDE SEQUENCE.
RA Ma F.W., Zhang Y.Z., Zhang J.K., Liang D.;
RT "Molecular cloning of glutathione-dependent dehydroascorbate reductase gene
RT of Royal Gala.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RXI09015.1, ECO:0000313|Proteomes:UP000290289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. HFTH1 {ECO:0000313|Proteomes:UP000290289};
RC TISSUE=Young leaf {ECO:0000313|EMBL:RXI09015.1};
RA Hu J.;
RT "A high-quality apple genome assembly.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 glutathione + L-dehydroascorbate = glutathione disulfide +
CC L-ascorbate; Xref=Rhea:RHEA:24424, ChEBI:CHEBI:38290,
CC ChEBI:CHEBI:57925, ChEBI:CHEBI:58297, ChEBI:CHEBI:58539; EC=1.8.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000509};
CC -!- SIMILARITY: Belongs to the GST superfamily. DHAR family.
CC {ECO:0000256|ARBA:ARBA00024194}.
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DR EMBL; DQ322706; ABG49123.1; -; mRNA.
DR EMBL; RDQH01000327; RXI09015.1; -; Genomic_DNA.
DR RefSeq; NP_001281055.1; NM_001294126.1.
DR AlphaFoldDB; Q0ZNW5; -.
DR STRING; 3750.Q0ZNW5; -.
DR EnsemblPlants; mRNA:MD01G0094700; mRNA:MD01G0094700; MD01G0094700.
DR GeneID; 103433463; -.
DR Gramene; mRNA:MD01G0094700; mRNA:MD01G0094700; MD01G0094700.
DR KEGG; mdm:103433463; -.
DR OrthoDB; 103277at2759; -.
DR Proteomes; UP000290289; Chromosome 1.
DR GO; GO:0045174; F:glutathione dehydrogenase (ascorbate) activity; IEA:UniProtKB-EC.
DR GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0033355; P:ascorbate glutathione cycle; IEA:InterPro.
DR CDD; cd03201; GST_C_DHAR; 1.
DR CDD; cd00570; GST_N_family; 1.
DR Gene3D; 1.20.1050.10; -; 1.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR InterPro; IPR044627; DHAR1/2/3/4.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR040079; Glutathione_S-Trfase.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR44420; GLUTATHIONE S-TRANSFERASE DHAR2-RELATED; 1.
DR PANTHER; PTHR44420:SF6; REDUCTASE, PUTATIVE-RELATED; 1.
DR Pfam; PF13410; GST_C_2; 1.
DR Pfam; PF13409; GST_N_2; 1.
DR SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR SFLD; SFLDG00358; Main_(cytGST); 1.
DR SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR SUPFAM; SSF52833; Thioredoxin-like; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 2: Evidence at transcript level;
KW Oxidoreductase {ECO:0000313|EMBL:ABG49123.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000290289}.
FT DOMAIN 10..88
FT /note="GST N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50404"
FT DOMAIN 66..213
FT /note="GST C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50405"
SQ SEQUENCE 213 AA; 23520 MW; 6CC6CBD1D464E0DE CRC64;
MALEVAAKAA AGAPDLLGDC PFCQRVTLTL EEKKVPYKLH LINLSDKPKW FTEVNPEGKV
PVVKFDDKWV PDSDVIVGII EEKYPEPSLK TPPEFASVGS KILGSFVTFL KSKDPGDGSE
QALLTELKAL DEHLKAHGPY IAGEKVTAAD LSLAPKLYHL KVALGHFKKW TVPADLAHYH
KYTELLFSRE SFVKTAPADE KYVIAGWEPK VNP
//
