ID   Q19VD5_CVHK1            Unreviewed;       928 AA.
AC   Q19VD5;
DT   11-JUL-2006, integrated into UniProtKB/TrEMBL.
DT   11-JUL-2006, sequence version 1.
DT   28-JUN-2023, entry version 44.
DE   RecName: Full=ORF1ab polyprotein {ECO:0000256|PROSITE-ProRule:PRU01344};
DE   Flags: Fragment;
OS   Human coronavirus HKU1 (HCoV-HKU1).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Embecovirus.
OX   NCBI_TaxID=290028 {ECO:0000313|EMBL:ABD91885.1};
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1] {ECO:0000313|EMBL:ABD91885.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=N17 {ECO:0000313|EMBL:ABD91885.1};
RX   PubMed=16757599; DOI=10.1128/JCM.02614-05;
RA   Lau S.K., Woo P.C., Yip C.C., Tse H., Tsoi H.W., Cheng V.C., Lee P.,
RA   Tang B.S., Cheung C.H., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Yuen K.Y.;
RT   "Coronavirus HKU1 and other coronavirus infections in Hong Kong.";
RL   J. Clin. Microbiol. 44:2063-2071(2006).
CC   -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC       multifunctional protein: it contains the activities necessary for the
CC       transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC       and progeny virion RNA as well as proteinases responsible for the
CC       cleavage of the polyprotein into functional products.
CC       {ECO:0000256|ARBA:ARBA00003368}.
CC   -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family.
CC       {ECO:0000256|ARBA:ARBA00008087, ECO:0000256|PROSITE-ProRule:PRU01344}.
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DR   EMBL; DQ422733; ABD91885.1; -; Genomic_RNA.
DR   SMR; Q19VD5; -.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   CDD; cd21593; HCoV_HKU1-like_RdRp; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR044863; NIRAN.
DR   InterPro; IPR047570; NSP12_IF_CoV.
DR   InterPro; IPR046441; RdRp_CoV.
DR   InterPro; IPR044347; RdRp_HCoV_HKU1-like.
DR   InterPro; IPR009469; RdRp_N_CoV.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   Pfam; PF06478; CoV_RPol_N; 1.
DR   Pfam; PF00680; RdRP_1; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   PROSITE; PS52000; COV_NSP12_IF; 1.
DR   PROSITE; PS51948; COV_NSP12_RDRP; 1.
DR   PROSITE; PS51947; NIRAN; 1.
PE   3: Inferred from homology;
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW   ECO:0000313|EMBL:ABD91885.1}; Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          6..261
FT                   /note="NiRAN"
FT                   /evidence="ECO:0000259|PROSITE:PS51947"
FT   DOMAIN          262..360
FT                   /note="Nsp12 Interface"
FT                   /evidence="ECO:0000259|PROSITE:PS52000"
FT   DOMAIN          361..928
FT                   /note="Nsp12 RNA-dependent RNA polymerase"
FT                   /evidence="ECO:0000259|PROSITE:PS51948"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:ABD91885.1"
FT   NON_TER         928
FT                   /evidence="ECO:0000313|EMBL:ABD91885.1"
SQ   SEQUENCE   928 AA;  106593 MW;  DF1313CE70446E8F CRC64;
     SKDLNFLNRV RGTSVNARLV PCASGLSTDV QLRAFDICNT NRAGIGLYYK VNCCRFQRID
     DDGNKLDKFF VVKRTNLEVY NKEKTYYELT KSCGVVAEHD FFTFDIDGSR VPHIVRRNLS
     KYTMLDLCYA LRHFDRNDCS ILCEILCEYA DCKESYFSKK DWYDFVENPD IINIYKKLGP
     IFNRALLNTV IFADTLVEVG LVGVLTLDNQ DLYGQWYDFG DFIQTAPGFG VAVADSYYSY
     MMPMLTMCHV LDCELFVNDS YRQFDLVQYD FTDYKLELFN KYFKYWGMKY HPNTVDCDND
     RCIIHCANFN ILFSMVLPNT CFGPLVRQIF VDGVPFVVSI GYHYKELGVV MNLDVDTHRY
     RLSLKDLLLY AADPAMHVAS ASALLDLRTC CFSVAAITSG IKFQTVKPGN FNQDFYEFVK
     SKGLFKEGST VDLKHFFFTQ DGNAAITDYN YYKYNLPTMV DIKQLLFVLE VVYKYFEIYD
     GGCIPASQVI VNNYDKSAGY PFNKFGKARL YYEALSFEEQ NEIYAYTKRN VLPTLTQMNL
     KYAISAKNRA RTVAGVSILS TMTGRMFHQK CLKSIAATRG VPVVIGTTKF YGGWDDMLRH
     LIKDVDNPVL MGWDYPKCDR AMPNILRIVS SLVLARKHEF CCSHGDRFYR LANECAQVLS
     EIVMCGGCYY VKPGGTSSGD ATTAFANSVF NICQAVTANV CSLMACNGHK IEDLSIRNLQ
     KRLYSNVYRT DYVDYTFVNE YYEFLCKHFS MMILSDDGVV CYNSDYANKG YIANISAFQQ
     VLYYQNNVFM SESKCWVEND ITNGPHEFCS QHTMLVKIDG DYVYLPYPDP SRILGAGCFV
     DDLLKTDSVL LIERFVSLAI DAYPLVYHEN EEYQKVFRVY LEYIKKLYND LGTQILDSYS
     VILSTCDGLK FTEESFYKNM YLKSAVMQ
//