ID DCL2_COCIM Reviewed; 1435 AA.
AC Q1DW80; J3KG17;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 3.
DT 01-MAY-2013, entry version 46.
DE RecName: Full=Dicer-like protein 2;
DE Includes:
DE RecName: Full=Endoribonuclease DCL2;
DE EC=3.1.26.-;
DE Includes:
DE RecName: Full=ATP-dependent helicase DCL2;
DE EC=3.6.4.-;
GN Name=DCL2; ORFNames=CIMG_05433;
OS Coccidioides immitis (strain RS) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; mitosporic Onygenales; Coccidioides.
OX NCBI_TaxID=246410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RS;
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J.,
RA Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E.,
RA Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M.,
RA Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N., Orbach M.J.,
RA Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens
RT Coccidioides and their relatives.";
RL Genome Res. 19:1722-1731(2009).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=RS;
RX PubMed=20516208; DOI=10.1101/gr.103911.109;
RA Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA Taylor J.W., Rounsley S.D.;
RT "Population genomic sequencing of Coccidioides fungi reveals recent
RT hybridization and transposon control.";
RL Genome Res. 20:938-946(2010).
CC -!- FUNCTION: Dicer-like endonuclease involved in cleaving double-
CC stranded RNA in the RNA interference (RNAi) pathway. Produces 21
CC to 25 bp dsRNAs (siRNAs) which target the selective destruction of
CC homologous RNAs leading to sequence-specific suppression of gene
CC expression, called post-transcriptional gene silencing (PTGS).
CC Part of a broad host defense response against viral infection and
CC transposons (By similarity).
CC -!- COFACTOR: Magnesium or manganese (By similarity).
CC -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC -!- SIMILARITY: Contains 1 Dicer dsRNA-binding fold domain.
CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC -!- SIMILARITY: Contains 1 helicase C-terminal domain.
CC -!- SIMILARITY: Contains 2 RNase III domains.
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DR EMBL; GG704914; EAS34630.2; -; Genomic_DNA.
DR RefSeq; XP_001246213.1; XM_001246212.1.
DR ProteinModelPortal; Q1DW80; -.
DR GeneID; 4563993; -.
DR KEGG; cim:CIMG_05654; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0050688; P:regulation of defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 1.10.1520.10; -; 3.
DR InterPro; IPR005034; Dicer_dsRNA_binding_fold.
DR InterPro; IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
DR InterPro; IPR001159; Ds-RNA-bd.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR000999; RNase_III_dom.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF03368; dsRNA_bind; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00636; Ribonuclease_3; 2.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00535; RIBOc; 2.
DR SUPFAM; SSF69065; RNase_III; 2.
DR PROSITE; PS51327; DICER_DSRBF; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS00517; RNASE_3_1; 2.
DR PROSITE; PS50142; RNASE_3_2; 2.
PE 3: Inferred from homology;
KW Antiviral defense; Antiviral protein; ATP-binding; Complete proteome;
KW Helicase; Hydrolase; Magnesium; Manganese; Metal-binding;
KW Nucleotide-binding; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1 1435 Dicer-like protein 2.
FT /FTId=PRO_0000306791.
FT DOMAIN 54 234 Helicase ATP-binding.
FT DOMAIN 400 564 Helicase C-terminal.
FT DOMAIN 591 684 Dicer dsRNA-binding fold.
FT DOMAIN 956 1099 RNase III 1.
FT DOMAIN 1141 1323 RNase III 2.
FT NP_BIND 67 74 ATP (By similarity).
FT MOTIF 175 178 DEAH box.
FT METAL 1178 1178 Magnesium or manganese (By similarity).
FT METAL 1309 1309 Magnesium or manganese (By similarity).
FT METAL 1312 1312 Magnesium or manganese (By similarity).
FT SITE 1305 1305 Important for activity (By similarity).
SQ SEQUENCE 1435 AA; 162531 MW; A7633F0AFD7D44FD CRC64;
MSAHTTAEQS PKRRRIHLDN DSKMHFSSIL EDGTSKSQEA LLPPRRARGY QLEMLSESLR
QNIIVAMDTG SGKTEIAILR IQRELERCPA HKFVWFMAPT VALVEQQHSA ISKQLPAFQT
RLLTGAANVS HWSTKKIWDD ILLNIRIVIS TPQVLLDALS NGFVDLHTIS LLVFDEAHHC
VRDAPANRIM RDFYHYHRQE EGTDGLPHIL GLTASPTTRA RQTDLEVLEI NLNAVCVTPK
MHREEMMQFV HMPEYRSIEY QPDVQNFSSI VEKLSVIINE LDIENDPFVK FMRRRNDLKS
RQRLLDALEN KKTPCLDQLK RCLRRSRVIH RELGPWASER FLTRCIMGLK SKQTNASGPQ
WADWDREDNS YMLNVLSQVV SSTEMGVQNP PDELSRKVHK LIDFLVLEHV NGSIGIVFAE
ERTIVIMLAQ LLSLHPRTKH IKTTAFLGSS ASVSRKSDIT ELHNPIDQST AIDDLRTGKK
DLIIATAVLE EGIDVPICDL VICFDLPKDL RSFIQRRGRA RKKGSKFALF LHSEDRATSS
ELHLMEKTMK QLYLENKRAL EHIQYLENVE EEGYDGFRVA STGALLTLSN ARNHLSHFCG
TLSAEFIATD PEFVLEGDDT TGFSAKVILP SFLDPKLREF RGILLWKTEK MAKRDASFQA
YVALYEAGLV NDYLMPAHHH IDDEDGLEQV EKRPSFAKAL GSLNPWAAIA KKWREAKHFY
QNLIEISAGA QAFPPMVMVL PVELPCDISF RLFWNEHSTL LVSVKRGGQD FAADLIRLAA
DTTSILLSSL FSQKMVPGSL DFSWLFLPQM ESIPSAIREW CDSVTGTISL HDIRDCDMAG
FENPGLVRPM DNTARPCTFE KLVWRKYVPA ETSDGASLSE QVTYDREVPH IEGRVWPKRT
DFLHRLEPSN TSKAHHTAKC FYPANNCSVD RLPVEYSQFA LFIPCLIHSI ENYFIANELA
QTILHPVGFS NLSLVLTAIS SSAAREASNY QRLEFLGDSL LKLHTSIQLA ADHPLWPEGR
LTMRKGNIVS NGYLANAALQ TGLDKFILTK PFTGAKWRPS YNTDHINTGD MTEPTREMST
KVLADVVEAL IGAANIDGGE NKILNCLKIF IPDIKWSPLN ECVNILHHQE DSFSDENNNM
LSEIEGLVGY TFKKKPLLLA AVTHPSSKGS GHSYQRLEFV GDSILDIIVV QELFESPRCF
HHFDMHLMRT ALVNADFLAF LCMNAYREED RGEAVENSKR RVTVAMTKRR AYLWGFMKHS
ASWDIVNAQQ RAAKQYEKLH EEIDEKLRSS KTYPWTLLCR LDAAKFFSDI VESILGAIFI
DSQGSMPACR IFLERIGLIP YLKRVLSEDL DLMHPKERLG LLAGTLSVKY ETKRTQGVEP
QRWECAARVG DEEVVRVDCR VSRVDAETTA AEAAVAILKT RKLQSEASNK VAECD
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