ID Q1HXP9_CLOPF Unreviewed; 398 AA.
AC Q1HXP9;
DT 13-JUN-2006, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2006, sequence version 1.
DT 28-JUN-2023, entry version 63.
DE RecName: Full=Phospholipase C {ECO:0000256|ARBA:ARBA00018391};
DE EC=3.1.4.3 {ECO:0000256|ARBA:ARBA00012018};
DE AltName: Full=Phosphatidylcholine cholinephosphohydrolase {ECO:0000256|ARBA:ARBA00031285};
GN Name=plc {ECO:0000313|EMBL:ABA64012.1};
OS Clostridium perfringens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1502 {ECO:0000313|EMBL:ABA64012.1};
RN [1] {ECO:0000313|EMBL:ABA64012.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NRRL B-23819 {ECO:0000313|EMBL:ABA63941.1}, NRRL B-23821
RC {ECO:0000313|EMBL:ABA63942.1}, NRRL B-23824
RC {ECO:0000313|EMBL:ABA63944.1}, and NRRL B-23825
RC {ECO:0000313|EMBL:ABA64012.1};
RX PubMed=16489222; DOI=10.1534/genetics.105.054601;
RA Rooney A.P., Swezey J.L., Friedman R., Hecht D.W., Maddox C.W.;
RT "Analysis of core housekeeping and virulence genes reveals cryptic lineages
RT of Clostridium perfringens that are associated with distinct disease
RT presentations.";
RL Genetics 172:2081-2092(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycerol + H(+) + phosphocholine; Xref=Rhea:RHEA:10604,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3;
CC Evidence={ECO:0000256|ARBA:ARBA00000291};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00152}.
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DR EMBL; DQ183948; ABA63941.1; -; Genomic_DNA.
DR EMBL; DQ183949; ABA63942.1; -; Genomic_DNA.
DR EMBL; DQ183951; ABA63944.1; -; Genomic_DNA.
DR EMBL; DQ184019; ABA64012.1; -; Genomic_DNA.
DR AlphaFoldDB; Q1HXP9; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0034480; F:phosphatidylcholine phospholipase C activity; IEA:UniProtKB-EC.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00113; PLAT; 1.
DR CDD; cd10981; ZnPC_S1P1; 1.
DR Gene3D; 1.10.575.10; P1 Nuclease; 1.
DR Gene3D; 2.60.60.20; PLAT/LH2 domain; 1.
DR InterPro; IPR001024; PLAT/LH2_dom.
DR InterPro; IPR036392; PLAT/LH2_dom_sf.
DR InterPro; IPR008947; PLipase_C/P1_nuclease_dom_sf.
DR InterPro; IPR029002; PLPC/GPLD1.
DR InterPro; IPR001531; Zn_PLipaseC.
DR Pfam; PF01477; PLAT; 1.
DR Pfam; PF00882; Zn_dep_PLPC; 1.
DR PRINTS; PR00479; PRPHPHLPASEC.
DR SMART; SM00770; Zn_dep_PLPC; 1.
DR SUPFAM; SSF49723; Lipase/lipooxygenase domain (PLAT/LH2 domain); 1.
DR SUPFAM; SSF48537; Phospholipase C/P1 nuclease; 1.
DR PROSITE; PS50095; PLAT; 1.
DR PROSITE; PS00384; PROKAR_ZN_DEPEND_PLPC_1; 1.
DR PROSITE; PS51346; PROKAR_ZN_DEPEND_PLPC_2; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cytolysis {ECO:0000256|ARBA:ARBA00022852};
KW Hemolysis {ECO:0000256|ARBA:ARBA00022735};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Toxin {ECO:0000256|ARBA:ARBA00022656};
KW Virulence {ECO:0000256|ARBA:ARBA00023026};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..398
FT /note="Phospholipase C"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5038288191"
FT DOMAIN 26..278
FT /note="Zn-dependent PLC"
FT /evidence="ECO:0000259|PROSITE:PS51346"
FT DOMAIN 284..398
FT /note="PLAT"
FT /evidence="ECO:0000259|PROSITE:PS50095"
SQ SEQUENCE 398 AA; 45476 MW; 2E774A7469175972 CRC64;
MKRKICKALI CAALATSLWA GASTKVYAWD GKIDGTGTHA MIVTQGVSIL ENDLSKNEPE
SVRKNLEILK ENMHELQLGS TYPDYDKNAY DLYQDHFWDP DTDNNFSKDN SWYLAYSIPD
TGESQIRKFS ALARYEWQRG NYKQATFYLG EAMHYFGDID TPYHPANVTA VDSAGHVKFE
TFAEERKEQY KINTAGCKTN EDFYADILKN KDFNAWSKEY ARGFAKTGKS IYYSHASMSH
SWDDWDYAAK VTLANSQKGT AGYIYRFLHD VSEGNDSSVG KNVKELVAYI STSGEKDAGT
DDYMYFGIKT KDGKTQEWEM DNPGNDFMTG SKDTYTFKLK DENLKIDDIQ NMWIRKRKYT
AFPDAYKPEN IKVIANGKVV VDKDINEWIS GNSTYNIK
//
