ID MURD_RALME Reviewed; 504 AA.
AC Q1LIM4;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 01-MAY-2013, entry version 56.
DE RecName: Full=UDP-N-acetylmuramoylalanine--D-glutamate ligase;
DE EC=6.3.2.9;
DE AltName: Full=D-glutamic acid-adding enzyme;
DE AltName: Full=UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase;
GN Name=murD; OrderedLocusNames=Rmet_3130;
OS Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=266264;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CH34 / ATCC 43123 / DSM 2839;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Mergeay M., Benotmane M.A.,
RA Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S.,
RA van der Lelie D., Richardson P.;
RT "Complete sequence of the chromosome of Ralstonia metallidurans
RT CH34.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cell wall formation. Catalyzes the addition of glutamate
CC to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
CC (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + UDP-N-acetylmuramoyl-L-alanine +
CC glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-
CC glutamate.
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the MurCDEF family.
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DR EMBL; CP000352; ABF10002.1; -; Genomic_DNA.
DR RefSeq; YP_585271.1; NC_007973.1.
DR ProteinModelPortal; Q1LIM4; -.
DR STRING; 266264.Rmet_3130; -.
DR EnsemblBacteria; ABF10002; ABF10002; Rmet_3130.
DR GeneID; 4039959; -.
DR KEGG; rme:Rmet_3130; -.
DR PATRIC; 20290895; VBIRalMet4734_3522.
DR eggNOG; COG0771; -.
DR HOGENOM; HOG000049427; -.
DR KO; K01925; -.
DR OMA; RCGELDV; -.
DR ProtClustDB; PRK02006; -.
DR BioCyc; CMET266264:GJ5G-3339-MONOMER; -.
DR UniPathway; UPA00219; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004326; F:tetrahydrofolylpolyglutamate synthase activity; IEA:InterPro.
DR GO; GO:0008764; F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity; IEA:HAMAP.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR GO; GO:0046901; P:tetrahydrofolylpolyglutamate biosynthetic process; IEA:GOC.
DR Gene3D; 3.40.1190.10; -; 2.
DR Gene3D; 3.40.50.720; -; 1.
DR Gene3D; 3.90.190.20; -; 1.
DR HAMAP; MF_00639; MurD; 1; -.
DR InterPro; IPR018109; Folylpolyglutamate_synth_CS.
DR InterPro; IPR004101; Mur_ligase_C.
DR InterPro; IPR013221; Mur_ligase_cen.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR005762; UDP-N-AcMur-Glu_ligase.
DR PANTHER; PTHR23135:SF2; PTHR23135:SF2; 1.
DR Pfam; PF02875; Mur_ligase_C; 1.
DR Pfam; PF08245; Mur_ligase_M; 1.
DR SUPFAM; SSF53244; Mur_ligase_C; 1.
DR SUPFAM; SSF53623; Mur_ligase_cen; 1.
DR TIGRFAMs; TIGR01087; murD; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; Cell shape;
KW Cell wall biogenesis/degradation; Complete proteome; Cytoplasm;
KW Ligase; Nucleotide-binding; Peptidoglycan synthesis.
FT CHAIN 1 504 UDP-N-acetylmuramoylalanine--D-glutamate
FT ligase.
FT /FTId=PRO_0000257222.
FT NP_BIND 129 135 ATP (Potential).
SQ SEQUENCE 504 AA; 53563 MW; 714A1E689FD7530F CRC64;
MFGVLQKPHV LVLGLGESGL AMARWCGLNG CRVRVADTRE APANLVFLQA ELTTAQFMGG
QFTENLLDDI GLVAISPGLS PLEPNTRALL EAAQARSIPV WGEIELFAQA IGYLEATSGY
APRVLAITGT NGKTTTTALT GRLIERAGKT VGVAGNISPS ALDKLSACIA SATLPDVWVL
ELSSFQLEYT FSLAPHAATV LNVTQDHLDW HGSMEAYAAA KARIFGPAEK GCLQVLNRQD
PLTMNMARRG TTLVTFGTDL PETPGSYGVL REGGMPWLVL AEPDTEADAE QKPRRRKKDD
VAADAVVPVR HKRLMPADAL HIRGMHNATN AMAALALCRA IDLPLNALLH GLREYRGEPH
RVEWVATIDE VEYFDDSKGT NVGATVAALS GLDKHVVLIA GGEGKGQDFS PLVAPVAQYA
RAVVLIGKDA GALREALGAT GKPLIDAGSL EEAVEKSASL AEAGDVVLLS PACASLDMFR
NYVHRAQVFR GAVEELALSR GIMP
//
