ID Q1M2F0_9POXV Unreviewed; 479 AA.
AC Q1M2F0;
DT 30-MAY-2006, integrated into UniProtKB/TrEMBL.
DT 30-MAY-2006, sequence version 1.
DT 08-NOV-2023, entry version 62.
DE RecName: Full=Poly(A) polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00017061, ECO:0000256|PIRNR:PIRNR015693};
DE EC=2.7.7.19 {ECO:0000256|ARBA:ARBA00012388, ECO:0000256|PIRNR:PIRNR015693};
DE AltName: Full=Poly(A) polymerase large subunit {ECO:0000256|PIRNR:PIRNR015693};
GN ORFNames=List053 {ECO:0000313|EMBL:ABD52515.1}, synVACV_067
GN {ECO:0000313|EMBL:QIB98553.1}, VACAC2_067
GN {ECO:0000313|EMBL:AAR17899.1}, VACli_067
GN {ECO:0000313|EMBL:QIB98318.1};
OS Vaccinia virus.
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX NCBI_TaxID=10245 {ECO:0000313|EMBL:AAR17899.1, ECO:0000313|Proteomes:UP000125606};
RN [1] {ECO:0000313|EMBL:ABD52515.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lister {ECO:0000313|EMBL:ABD52515.1};
RX PubMed=17554021; DOI=10.1099/vir.0.82708-0;
RA Garcel A., Crance J.M., Drillien R., Garin D., Favier A.L.;
RT "Genomic sequence of a clonal isolate of the vaccinia virus Lister strain
RT employed for smallpox vaccination in France and its comparison to other
RT orthopoxviruses.";
RL J. Gen. Virol. 88:1906-1916(2007).
RN [2] {ECO:0000313|EMBL:AAR17899.1, ECO:0000313|Proteomes:UP000125606}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Acambis {ECO:0000313|EMBL:AAR17899.1};
RX PubMed=18037545; DOI=10.1016/j.vaccine.2007.10.040;
RA Osborne J.D., Da Silva M., Frace A.M., Sammons S.A., Olsen-Rasmussen M.,
RA Upton C., Buller R.M., Chen N., Feng Z., Roper R.L., Liu J.,
RA Pougatcheva S., Chen W., Wohlhueter R.M., Esposito J.J.;
RT "Genomic differences of Vaccinia virus clones from Dryvax smallpox vaccine:
RT the Dryvax-like ACAM2000 and the mouse neurovirulent Clone-3.";
RL Vaccine 25:8807-8832(2007).
RN [3] {ECO:0000313|EMBL:QKE61196.1, ECO:0000313|Proteomes:UP000501395}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VK01 {ECO:0000313|EMBL:QKE61196.1};
RA Duggan A.T., Klunk J., Porter A.F., Dhody A.N., Hicks R., Smith G.L.,
RA Humphreys M., McCollum A.M., Davidson W.B., Wilkins K., Li Y., Burke A.,
RA Polasky H., Flanders L., Poinar D., Raphenya A.R., Lau T.T.Y., Alcock B.,
RA McArthur A.G., Golding G.B., Holmes E.C., Poinar H.N.;
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:QKE59683.1, ECO:0000313|Proteomes:UP000503040}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Acambis/2019 {ECO:0000313|EMBL:QKE59683.1,
RC ECO:0000313|Proteomes:UP000503040};
RA Martin R.M., Burke K., Verma D., Xie H., Langer J., Schlaberg R.,
RA Swaminathan S., Hanson K.E.;
RT "Contact Transmission of Vaccinia to an Infant Diagnosed by Viral Culture
RT and Metagenomic Sequencing.";
RL Open Forum Infect. Dis. 7:0-ofaa111(2020).
RN [5] {ECO:0000313|EMBL:QIB98318.1, ECO:0000313|Proteomes:UP000502965}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VACV_li {ECO:0000313|EMBL:QIB98318.1,
RC ECO:0000313|Proteomes:UP000502965};
RA Noyce R.S., Evans D.H.;
RL Submitted (JAN-2020) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:QIB98553.1, ECO:0000313|Proteomes:UP000501854}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TNX-1200 {ECO:0000313|EMBL:QIB98553.1,
RC ECO:0000313|Proteomes:UP000501854};
RA Noyce R.S., Lederman S., Evans D.H.;
RL Submitted (JAN-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Polymerase that creates the 3'-poly(A) tail of mRNA's.
CC {ECO:0000256|ARBA:ARBA00003054, ECO:0000256|PIRNR:PIRNR015693,
CC ECO:0000256|RuleBase:RU004458}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173115; EC=2.7.7.19;
CC Evidence={ECO:0000256|ARBA:ARBA00024620,
CC ECO:0000256|PIRNR:PIRNR015693, ECO:0000256|RuleBase:RU004458};
CC -!- SUBUNIT: Heterodimer of a large (catalytic) subunit and a small
CC (regulatory) subunit. {ECO:0000256|ARBA:ARBA00011405,
CC ECO:0000256|PIRNR:PIRNR015693, ECO:0000256|RuleBase:RU004458}.
CC -!- SIMILARITY: Belongs to the poxviridae poly(A) polymerase catalytic
CC subunit family. {ECO:0000256|ARBA:ARBA00006268,
CC ECO:0000256|PIRNR:PIRNR015693, ECO:0000256|RuleBase:RU004458}.
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DR EMBL; AY313847; AAR17899.1; -; Genomic_DNA.
DR EMBL; DQ121394; ABD52515.1; -; Genomic_DNA.
DR EMBL; MN974380; QIB98318.1; -; Genomic_DNA.
DR EMBL; MN974381; QIB98553.1; -; Genomic_DNA.
DR EMBL; MT227314; QKE59683.1; -; Genomic_DNA.
DR EMBL; MN369532; QKE61196.1; -; Genomic_DNA.
DR Proteomes; UP000125606; Genome.
DR Proteomes; UP000180811; Genome.
DR Proteomes; UP000501395; Genome.
DR Proteomes; UP000501854; Genome.
DR Proteomes; UP000502965; Genome.
DR Proteomes; UP000503040; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1990817; F:poly(A) RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-UniRule.
DR CDD; cd20919; polyA_pol_Pox; 1.
DR Gene3D; 1.20.1270.320; Poxvirus poly(A) polymerase, N domain; 1.
DR Gene3D; 3.30.460.60; Poxvirus poly(A) polymerase, nucleotidyltransferase domain; 1.
DR InterPro; IPR004976; PolyA_pol_cat_Poxvir.
DR InterPro; IPR037265; PolyA_pol_cat_sf.
DR InterPro; IPR024231; PolyA_pol_nucTrfase_Poxvir.
DR InterPro; IPR038419; PolyA_pol_nucTrfase_sf_Poxvir.
DR InterPro; IPR024397; Poxvirus_polyA_pol_cat_C.
DR InterPro; IPR024398; Poxvirus_polyA_pol_cat_N.
DR InterPro; IPR038337; Poxvirus_polyA_pol_cat_N_sf.
DR Pfam; PF03296; Pox_polyA_pol; 1.
DR Pfam; PF12629; Pox_polyA_pol_C; 1.
DR Pfam; PF12630; Pox_polyA_pol_N; 1.
DR PIRSF; PIRSF015693; VAC-48L_nuct; 2.
DR SUPFAM; SSF160957; Poly(A) polymerase catalytic subunit-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR015693};
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Early protein {ECO:0000256|ARBA:ARBA00022518};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664,
KW ECO:0000256|PIRNR:PIRNR015693};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR015693};
KW Nucleotidyltransferase {ECO:0000313|EMBL:ABD52515.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|PIRNR:PIRNR015693};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR015693}.
FT DOMAIN 15..117
FT /note="Poxvirus poly(A) polymerase catalytic subunit N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12630"
FT DOMAIN 132..279
FT /note="Poly(A) polymerase nucleotidyltransferase"
FT /evidence="ECO:0000259|Pfam:PF03296"
FT DOMAIN 282..478
FT /note="Poxvirus poly(A) polymerase catalytic subunit C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12629"
FT ACT_SITE 202
FT /evidence="ECO:0000256|PIRSR:PIRSR015693-50"
FT ACT_SITE 204
FT /evidence="ECO:0000256|PIRSR:PIRSR015693-50"
SQ SEQUENCE 479 AA; 55523 MW; CE938663E69069FB CRC64;
MNRNPDQNTF PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK
KRFFSDVDTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP
NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN
KSCICYGSYS LYLINPNILY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY
MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK
DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY
LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL
CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF
//
