ID   Q1PS51_PERAM            Unreviewed;       273 AA.
AC   Q1PS51;
DT   16-MAY-2006, integrated into UniProtKB/TrEMBL.
DT   16-MAY-2006, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=Medium-chain acyl-CoA ligase ACSF2, mitochondrial {ECO:0000256|ARBA:ARBA00039638};
OS   Periplaneta americana (American cockroach) (Blatta americana).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blattoidea; Blattidae;
OC   Blattinae; Periplaneta.
OX   NCBI_TaxID=6978 {ECO:0000313|EMBL:ABE60814.1};
RN   [1] {ECO:0000313|EMBL:ABE60814.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Wu S., Wang S., Xu S.;
RT   "Immunological screening of Periplaneta americana cDNA expression libraries
RT   with serum immuned by the soluble protein antigens derived from male
RT   Periplaneta fuliginosa.";
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + CoA + octanoate = AMP + diphosphate + octanoyl-CoA;
CC         Xref=Rhea:RHEA:33631, ChEBI:CHEBI:25646, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386,
CC         ChEBI:CHEBI:456215; Evidence={ECO:0000256|ARBA:ARBA00035758};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a medium chain fatty acid + ATP + CoA = a medium-chain fatty
CC         acyl-CoA + AMP + diphosphate; Xref=Rhea:RHEA:48340,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:59558, ChEBI:CHEBI:90546, ChEBI:CHEBI:456215; EC=6.2.1.2;
CC         Evidence={ECO:0000256|ARBA:ARBA00036216};
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000256|ARBA:ARBA00006432}.
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DR   EMBL; DQ256397; ABE60814.1; -; mRNA.
DR   AlphaFoldDB; Q1PS51; -.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.980; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   PANTHER; PTHR43201; ACYL-COA SYNTHETASE; 1.
DR   PANTHER; PTHR43201:SF5; MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
PE   2: Evidence at transcript level;
FT   DOMAIN          2..129
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
FT   DOMAIN          180..255
FT                   /note="AMP-binding enzyme C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF13193"
SQ   SEQUENCE   273 AA;  31036 MW;  AF8B2BFCFD9A0F99 CRC64;
     MLEAIIQEKC HIVFGTPALY VDMIAVVREH GLKPSSLKVA MCGGAPCSLQ LIEDIKNVLN
     VENFVLGYGM TEAVSIFLPA PNESKDHTIV GRVAPHIEVK VVDKEGRMVP MGTPGQLCVR
     GYSVMLGYWN DEEKTREYMG RDGWAKTGDE FVLEEGGWGR IVGRIKDVII RIGDKIFPAE
     MEDFFMEHPD VMEVEVFGVP DPRVGEEICV YLRVRDGAIL TEDDIRNYCK DKLPEYRVPR
     YIRFVKKFER TVIGKIQKFR LLEQLKKELG LPD
//