ID TRMA_SACD2 Reviewed; 365 AA.
AC Q21KA1;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 1.
DT 01-MAY-2013, entry version 49.
DE RecName: Full=tRNA (uracil(54)-C(5))-methyltransferase;
DE EC=2.1.1.35;
DE AltName: Full=tRNA(m5U54)-methyltransferase;
DE Short=RUMT;
GN Name=trmA; OrderedLocusNames=Sde_1616;
OS Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Alteromonadaceae; Saccharophagus.
OX NCBI_TaxID=203122;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2-40 / ATCC 43961 / DSM 17024;
RX PubMed=18516288; DOI=10.1371/journal.pgen.1000087;
RA Weiner R.M., Taylor L.E. II, Henrissat B., Hauser L., Land M.,
RA Coutinho P.M., Rancurel C., Saunders E.H., Longmire A.G., Zhang H.,
RA Bayer E.A., Gilbert H.J., Larimer F., Zhulin I.B., Ekborg N.A.,
RA Lamed R., Richardson P.M., Borovok I., Hutcheson S.;
RT "Complete genome sequence of the complex carbohydrate-degrading marine
RT bacterium, Saccharophagus degradans strain 2-40 T.";
RL PLoS Genet. 4:E1000087-E1000087(2008).
CC -!- FUNCTION: Catalyzes the formation of 5-methyl-uridine at position
CC 54 (m5U54) in all tRNAs (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + uridine(54) in tRNA
CC = S-adenosyl-L-homocysteine + 5-methyluridine(54) in tRNA.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA M5U
CC methyltransferase family. TrmA subfamily.
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DR EMBL; CP000282; ABD80878.1; -; Genomic_DNA.
DR RefSeq; YP_527090.1; NC_007912.1.
DR ProteinModelPortal; Q21KA1; -.
DR STRING; 203122.Sde_1616; -.
DR EnsemblBacteria; ABD80878; ABD80878; Sde_1616.
DR GeneID; 3965723; -.
DR KEGG; sde:Sde_1616; -.
DR PATRIC; 23402153; VBISacDeg56404_1760.
DR eggNOG; COG2265; -.
DR HOGENOM; HOG000218626; -.
DR KO; K00557; -.
DR OMA; THRVERC; -.
DR ProtClustDB; PRK05031; -.
DR BioCyc; SDEG203122:GI2M-1666-MONOMER; -.
DR GO; GO:0030697; F:S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity; IEA:HAMAP.
DR HAMAP; MF_01011; tRNA_methyltr_TrmA; 1; -.
DR InterPro; IPR011869; TrmA_MeTrfase_proteobac.
DR InterPro; IPR010280; U5_MeTrfase.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR TIGRFAMs; TIGR02143; trmA_only; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; FALSE_NEG.
PE 3: Inferred from homology;
KW Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW Transferase; tRNA processing.
FT CHAIN 1 365 tRNA (uracil(54)-C(5))-methyltransferase.
FT /FTId=PRO_0000281456.
FT ACT_SITE 323 323 Nucleophile (By similarity).
FT BINDING 189 189 S-adenosyl-L-methionine (By similarity).
FT BINDING 217 217 S-adenosyl-L-methionine; via carbonyl
FT oxygen (By similarity).
FT BINDING 222 222 S-adenosyl-L-methionine (By similarity).
FT BINDING 238 238 S-adenosyl-L-methionine (By similarity).
FT BINDING 298 298 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 365 AA; 41469 MW; 81506946099C1853 CRC64;
MTTKLGQANP ANYQQQLDDK EKATKEQFSD FWQGDIEVHP SPPSHYRMRA EFKMWQQGAE
AFYAMYQPGE YKKPVTLEWE FSVGAEKIVE LMPKLLEIVN ASELLRKRLF QVEFLTTTTG
ESVTTLIYHK PLTEEWQAEA NALASSLNTH IIGRSKKQKI VLTQDYVEET LTISGTPFTY
KQIESGFTQP NAAVCQKMLQ WAVDATANLG GDLVELYCGN GNFTLPLSKN FDKVLATEVS
KTSVKAAEYN IEKNGCKNIT IARMSSEEFT EALNGVREFR RLKDIELDSY NFSTVFVDPP
RAGLDEDTEA LISRFDNIIY ISCNPDTLAN NLANLCKTHK VERFALFDQF PYTDHRECGV
ILTKK
//
