ID BARH2_DROME Reviewed; 645 AA.
AC Q24256; Q24257; Q4V712; Q9VX55;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 3.
DT 24-JAN-2024, entry version 184.
DE RecName: Full=Homeobox protein B-H2;
DE AltName: Full=Homeobox protein BarH2;
GN Name=B-H2; Synonyms=BarH2; ORFNames=CG5488;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S {ECO:0000269|PubMed:1346120}; TISSUE=Pupae;
RX PubMed=1346120; DOI=10.1101/gad.6.1.50;
RA Higashijima S., Kojima T., Michiue T., Ishimaru S., Emori Y., Saigo K.;
RT "Dual Bar homeo box genes of Drosophila required in two photoreceptor
RT cells, R1 and R6, and primary pigment cells for normal eye development.";
RL Genes Dev. 6:50-60(1992).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=1350558; DOI=10.1101/gad.6.6.1005;
RA Higashijima S., Michiue T., Emori Y., Saigo K.;
RT "Subtype determination of Drosophila embryonic external sensory organs by
RT redundant homeo box genes BarH1 and BarH2.";
RL Genes Dev. 6:1005-1018(1992).
RN [6] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10068639; DOI=10.1242/dev.126.7.1457;
RA Sato M., Kojima T., Michiue T., Saigo K.;
RT "Bar homeobox genes are latitudinal prepattern genes in the developing
RT Drosophila notum whose expression is regulated by the concerted functions
RT of decapentaplegic and wingless.";
RL Development 126:1457-1466(1999).
RN [7] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10648235; DOI=10.1242/dev.127.4.769;
RA Kojima T., Sato M., Saigo K.;
RT "Formation and specification of distal leg segments in Drosophila by dual
RT Bar homeobox genes, BarH1 and BarH2.";
RL Development 127:769-778(2000).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-593; SER-595 AND SER-602, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: B-H1 and B-H2 are regulated by members of the wg signaling
CC pathway; wg and dpp. B-H1 and B-H2 are coexpressed and functionally
CC required in R1 and R6 receptor cells and primary pigment cells for
CC normal eye development. Coexpression is also required for the fate
CC determination of external sensory organs, formation of notal
CC microchaetae, formation of presutural macrochaetae, antennal
CC development and for distal leg morphogenesis; segmentation and
CC specification of tarsal segments 3-5. {ECO:0000269|PubMed:10068639,
CC ECO:0000269|PubMed:10648235, ECO:0000269|PubMed:1346120,
CC ECO:0000269|PubMed:1350558}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: B-H1 and B-H2 are abundant in the eye-antenna
CC imaginal disk. Expressed in R1 and R6 cells throughout larval stage
CC until 30 hours after puparium formation, at which time expression is
CC seen in the anterior and posterior primary pigment cells. Coexpressed
CC in embryonic glial cells, neurons of the CNS and PNS, most latitudinal
CC anterior cells of the developing notum and the central circular region
CC of the leg and antennal imaginal disk throughout larval development.
CC {ECO:0000269|PubMed:10068639, ECO:0000269|PubMed:10648235,
CC ECO:0000269|PubMed:1346120, ECO:0000269|PubMed:1350558}.
CC -!- DEVELOPMENTAL STAGE: Coexpressed at high levels with B-H1 in embryo and
CC pupae and at low levels in larvae. {ECO:0000269|PubMed:1346120}.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. {ECO:0000305}.
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DR EMBL; M82884; AAA28383.2; -; mRNA.
DR EMBL; M82887; AAB59218.1; -; Genomic_DNA.
DR EMBL; M82885; AAB59218.1; JOINED; Genomic_DNA.
DR EMBL; M82886; AAB59218.1; JOINED; Genomic_DNA.
DR EMBL; AE014298; AAF48725.1; -; Genomic_DNA.
DR EMBL; BT022144; AAY51539.1; -; mRNA.
DR PIR; A41726; A41726.
DR PIR; S27806; S27806.
DR RefSeq; NP_523386.1; NM_078662.3.
DR AlphaFoldDB; Q24256; -.
DR SMR; Q24256; -.
DR BioGRID; 59051; 20.
DR IntAct; Q24256; 18.
DR STRING; 7227.FBpp0074203; -.
DR iPTMnet; Q24256; -.
DR PaxDb; 7227-FBpp0074203; -.
DR EnsemblMetazoa; FBtr0074429; FBpp0074203; FBgn0004854.
DR GeneID; 32723; -.
DR KEGG; dme:Dmel_CG5488; -.
DR UCSC; CG5488-RA; d. melanogaster.
DR AGR; FB:FBgn0004854; -.
DR CTD; 32723; -.
DR FlyBase; FBgn0004854; B-H2.
DR VEuPathDB; VectorBase:FBgn0004854; -.
DR eggNOG; KOG0488; Eukaryota.
DR GeneTree; ENSGT00940000169370; -.
DR HOGENOM; CLU_393440_0_0_1; -.
DR InParanoid; Q24256; -.
DR OMA; YRERHSP; -.
DR OrthoDB; 4204245at2759; -.
DR PhylomeDB; Q24256; -.
DR BioGRID-ORCS; 32723; 0 hits in 3 CRISPR screens.
DR ChiTaRS; B-H2; fly.
DR GenomeRNAi; 32723; -.
DR PRO; PR:Q24256; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0004854; Expressed in head epidermis primordium (Drosophila) and 32 other cell types or tissues.
DR ExpressionAtlas; Q24256; baseline and differential.
DR Genevisible; Q24256; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:FlyBase.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:UniProtKB.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0008057; P:eye pigment granule organization; IMP:UniProtKB.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IMP:UniProtKB.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0008052; P:sensory organ boundary specification; IMP:UniProtKB.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR PANTHER; PTHR24330:SF10; HOMEOBOX PROTEIN B-H1-RELATED; 1.
DR PANTHER; PTHR24330; HOMEOBOX PROTEIN BARH-LIKE; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW Reference proteome; Sensory transduction; Vision; Wnt signaling pathway.
FT CHAIN 1..645
FT /note="Homeobox protein B-H2"
FT /id="PRO_0000048833"
FT DNA_BIND 380..439
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 86..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..385
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 553..645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..165
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..385
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..595
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 605..632
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 593
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 595
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 602
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 121..123
FT /note="Missing (in Ref. 1; AAB59218)"
FT /evidence="ECO:0000305"
FT CONFLICT 229
FT /note="T -> P (in Ref. 1; AAB59218)"
FT /evidence="ECO:0000305"
FT CONFLICT 288..289
FT /note="Missing (in Ref. 1; AAB59218)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="S -> N (in Ref. 1; AAB59218)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 645 AA; 69951 MW; CF99E12F7ECBB133 CRC64;
MTTMPPEMSA TTAAPVGSAP SATAHHPAAV GGGMPRPASP AVGSNTTTAT ATTATRSRFM
ITDILAGAAA ASAAAAAAAA ALAAASSGGG RGSPTDSERE QSLVAQHHHH HQQQQQHHHH
QQQQQQQQHQ QAALQQYIVQ QQQLLRFERE REREREREHY RERHSPPGNN PYAHHPMPPH
LLAHFPPAHY AVLQQQQQQQ QQQQHPHPHH LQLERERLEA LHRHGHGLTG DPAQHLSHLS
HLSHQQHHPH LHHPMHDERS RSPLMLQQLG GNGGNNNNNN NNSSSASNNN NNNNNSASAN
SNIISGNSSS SNNNNGSGNG NMLLGGPGSS ISGDQASTID DSDSDDCGGK DDDGDDSMKN
GSSANGDSSS HLSLSLSKKQ RKARTAFTDH QLQTLEKSFE RQKYLSVQDR MELANKLELS
DCQVKTWYQN RRTKWKRQTA VGLELLAEAG NYAAFQRLYG GATPYLSAWP YAAAAAAQSP
HGATPSAIDI YYRQAAAAAA MQKPSLPASY RMYQSSIPPG MSLPGMPAPP PPGAAPMLSG
YYAAAAAAAA SAGAQQQQQQ PPAASRSPAT SQSANSEADC ERTSSSSRQR LITPSPPLNP
GSPPHRERIN EEEDRERDEE RDIERERERE RERDEDDEEE LALEV
//
