ID Q27W58_STRVO Unreviewed; 4787 AA.
AC Q27W58;
DT 04-APR-2006, integrated into UniProtKB/TrEMBL.
DT 04-APR-2006, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE SubName: Full=NigAVII {ECO:0000313|EMBL:ABC84471.1};
OS Streptomyces violaceusniger.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
OX NCBI_TaxID=68280 {ECO:0000313|EMBL:ABC84471.1};
RN [1] {ECO:0000313|EMBL:ABC84471.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 4137 {ECO:0000313|EMBL:ABC84471.1};
RX PubMed=17584617; DOI=10.1016/j.chembiol.2007.05.011;
RA Harvey B.M., Mironenko T., Sun Y., Hong H., Deng Z., Leadlay P.F.,
RA Weissman K.J., Haydock S.F.;
RT "Insights into polyether biosynthesis from analysis of the nigericin
RT biosynthetic gene cluster in Streptomyces sp. DSM4137.";
RL Chem. Biol. 14:703-714(2007).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; DQ354110; ABC84471.1; -; Genomic_DNA.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 3.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 6.10.140.1830; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF18369; PKS_DE; 3.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 6.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 34..460
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1503..1578
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1596..2022
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3070..3145
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3163..3589
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4625..4700
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 854..878
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2418..2441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3046..3067
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3970..3999
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3983..3999
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4787 AA; 507720 MW; B5EFAE90F08AAAB7 CRC64;
MAGNEEKLLD YLKRVTADLR QTQRRLKDVE SAGYEPVAIV GMSCRFPGGV RSPEELWELV
AAERDAISEL PTDRNWDVEN LFHPDPEHPG TSYTCEGGFI DDPAGFDAAF FGISPREALG
MAPQQRLALE ASWEAIEHAG VDPESLRGSR TGTFIGCDHL DYCSDASQVP EGSAGYFTIG
NSASVVSGRV AYTLGLEGAA VTVDTACSSS LVAMHLACQA IRQGECDMAL AGGVAVMSST
APFIGFSELR GLAPDGRSKA FSANSDGMTL AEGVGVLLLE RLADARRNGH QVLAVVRGTA
VNQDGASNGL TAPNGPSQQR VIRQALANAR LSPSDVDAVE AHGTGTTLGD PIEAQALLAT
YGQERPEGRP LWLGSIKSNI GHAQMAAGAA GVIKMVMAMR HEVLPASLHI DEPTPHVDWS
AGDIHLLTEA REWPRGEHPR RAGISSFGIS GTNAHVIVEQ APEEPPAAVG AGAGEPVEPV
EPVGGPVPWV VSARGAEALR GQARALAARV NADPGVSADE VGWSLLRSRT LFDHRAVVIG
QNREELVSGL EALAAGEPHP GVVEAGKAAE VAGQTVFLFS GQGSQRPGMG AELYNRFPIF
AEAFDEVCAL LDPHLEHPLR ELVFSREPEQ AALLDHTTYA QTGLFALHIA LARLLDSLGV
RPDTVIGHSI GEIAAAHIAG IFDLPDACHL VATRATLMGG LREGGGMATI AATPDELAED
LARYDGQVGI AALNTPGNTV ISGPTDLVTD ISVTWAAKGR KTRTLTVSHA FHSSLMDPIL
EPFKEAISGL TYHPPTIPLI SNLTGQPADE HITTPDYWAQ HIRQPVHFHP AITHIAPETG
IFLELGPDPT LTTATHHTLH HHTSTEQNPT NQPTPLITPA LTRKQPEWQA LTHALARLHT
AGVEIDWTSW FPADPAPRVV GLPTYAFQHQ RFWLAPPAAR TSFEGTAGDP AEARLWHAIE
ELDVDALSST LRLEKDSPGI DALLPALPVL STWRRQHRER STIDSWRYRA TWQQLPDPPT
PTLTGTWLVV VPAGQEEHAT VQVTVQALRA HGATALRHGV NAHDIDRAAL HERLARLTEE
FQPAGVISLL ALDTEPLIGH SAVPAGLGAT TALLQALDDA GIGTPLWCLT QGAVATGRGD
LLPHPGQSQI WGFGRVAALE YPQRWGGLID LPTTMTHHTA GRLAALLVPG QPEDQVAIRT
TGTYARRLAR VPAADTAPAS WEPSGTTLIT GGTGGLGAQI ARWLAHRGAP RLHLISRSGP
DAPGASELGE ELTALGTDTT ITACDASDRA ALQRLIDTVP AEHPLTAVFH TAGTMELGQI
GELEPGRLQS VLRSKALAAA HLHDLTQDLD LAAFVLFSSN AATWGSGQQA AYAAANTYLD
ALAEHRRARG LPATSLAWGP WGEAGMAADQ HTLSHLKRRG LSPLPTDLAI ASLHQALTHD
DTAVTIADVD WETFGRTFTA QRPSPFLTRL LPTSAEPTTP AATADDNPLR QQLGAAPTAQ
RHQILVRHIQ TLAATILGHP GAGRDSPGQP SRRLGFDSLT AVELRNQLAA STGLSLAPAL
VFDHPTPNAL ATYLGAELTG QRATVATHTS SVATDDEPIA IVGMACRYPG GVRSPEELWK
LVAAGRDAIG EMPTDRDWDL DTLFDPDPER LGTTYAREGG FLHDAAGFDA AFFGISPREA
LAMDPQQRLL LETAWETFES AGLDRDTLAG SNTGVFAGGT YQGYGASGSS SAREVEGYLL
AGGTPSVMSG RVAYAFGLEG PAVTVDTACS SSLVAMHLAA QALRQGECTM ALAGGVTVMA
TPTTFVEFSR QRGLAADGRC KPFALAADGT GWGEGAGLLL LERLSDARRN GHRVLAVIRG
SAVNQDGTSN GLTAPNGPSQ QRVIGQALTN ARVSPTEVDA VEGHGTGTTL GDPIEAQALL
ATYGRERPEG RPLWLGSIKS NIGHTQAASG VAGVIKMVMA MRHEVLPASL HIDEPTPHVD
WSAGDVQLLA EAREWPRGEH PRRAGISSFG ISGTNAHVIV EQAPEEPPAA VGADAGEPVE
PVERAESVEP VGGPVPWVVS ARGAEALRGQ VRALAARVYA DPGVSADEVG WSLLRSRTLF
DHRAVIIGQN REELTAGLEA LAAGEPHPGL VEPGEATDTT GKTVFLFSGQ GSQRPGMGAE
LYNRFPIFAE AFDEVCALLD PHLEHPLRQV VFDTSAEHAG LLDHTTYAQA GLFALHIALA
RLLDSVGVRP DTVIGHSIGE IAAAHIAGIF DLPDACHLVA TRATLMGQLP QGGSMATIAA
TPDELANDLA QHAGQVSIAV LNTPTNTVIS GPTELITNIS TTWAAKGRKT RTLTVSHAFH
SPLMDPILEP FKEAISGLTY HPPTIPLISN LTGQPADEHI TTPDYWAQHI RQPVHFHPAI
THTAPHTSTF LELGPDPTLT TATHHTLHHH TSTEQNPTNQ LTPLITSTLT RKQPERQALT
HALARLHTAG VEIDWTSWYP ATPTPRTIDL PTYAFQHQHY WLSSAPSSSP TPTEAALVDR
KFWEAVERED LEALAATIDS PADQQPMLSA VLPTLSAWRR HHREQSVINS WRYQIAWKRL
SAPSTPPDLS GTWLLIIPAG QSDHPAVETV AHALTTHGAT PIRHMLDTHT VDRDALADHL
TRLAMQGEPA GVLSLLALDE EPHPEYAAVP SGLAATTVLL QALGDAEIPA PLWCLTQGAV
ATGPGDPLPS PRQAQTWGLG RAAALEHPRR WGGLIDLPAV IDHHTADRLA ALLTPGRPED
QTAIRATGSY ARRLRKATAP TAAPRTWQPT GTTLITGGTG GLGAHVARWL ARLGAPHLHL
ISRSGPDAPG ASQLTQELID LGTAVTITAC DASDRAALQR LLDTIPAEHP LTTVIHAAGT
SDTELLADLG PERLEHVLGP KALAAAHLHE LTQELDLSAF VLFSSGAAAW GGSRQGAYAA
ANTYLDALAE HRRARGLPAT SLAWGPWGDA GMAADETALA FYGRRGLSPL SPELAVASLQ
HALDHRDTTI TVADIDWEKF PAAFTAQRPS PLLDDLITAI ESPAEPITGA STGTSLQQRL
SAGTPEQQHQ LLLERIQSLA ASILGHSGPD AIPPAQPFQE LGFDSLTAVE LRNQLATATG
IDLAPALVFD HPTPNALATY LRAELTGQQT AVAAHSPTTA AQDEPIAIVG MACRYPGDAH
SPQDLWELVI AHRDAIAQMP TDRNWDLDAL YDPDPDRAGT SYVREGGFLR HAAEFDPAFF
GISPREAIAM DPQQRLLLET AWETFESAGL DRDTLAGSNT GVFAGVTSQD YLSLTGDTAS
DVEGYVATGN IGSVVSGRVA YSFGLEGPAV TVDTACSSSL VAMHLAAQAL RQGECTMALA
GGVTVMATPG AFIEFSRQRG LAPDARCKPF AAAADGMVWG EGVGLVLLER LSDARRNGHR
VLAVVRGSAV NQDGTSNGLT APNGPSQQRV IRQALANARL SPSDVDAVEA HGTGTTLGDP
IEAQALLATY GQERPEGHPL WLGSIKSNIG HTQAAAGVAG VIKMVMAMRH ELLPASLHID
EPSPNVDWNA GDLRLLAETV PWPQGDRARR AGVSSFGISG TNAHLILEQA PEPAEPAPVS
NIAGVVPWVV SAQSDAALRG QARALAGHII GDPEVSAGEV GWSLLRSRTL FDHRAVVIGQ
NREELTAGLE ALAAGEPHPG LVEPGEATDT TGKTVFLFSG QGSQRPGMGT ELYNRFPIFA
EAFDEVCALL DPHLEHPLRQ VVFSREPEQA TLLDHTTYAQ AGLFALHIAL ARLLDSVGVR
PDTVIGHSIG EIAAAHIAGI FDLPDACHLV ATRATLMGQL PQGGGMATIG ATPEELAEDL
ARYDGQVSIA ALNTPTNTVI SGPTNLVTDV SATWAAKGRK TRTLTVSHAF HSPLMDPILE
PFKEAISGLT YHPPTIPLIS NLTGQPADEH ITTPDYWAQH IRQPVHFHPA ITHTAPHTST
FLELGPDPTL ATATHHTLHH HTSTEQNPTN QPTPLITSTL THKRPDTHAL THTLAQLHTS
GTTIDWTSWY PAIPTPRTID LPTYAFQHQH YWLAPPTPRA DSRLNGHNPA EARLWDAIEE
LDVDALASTL DLEKDSPGVG ALLPALPVLS TWRRRHRERS TIDSWRYRVA WKQVRDPATA
PTLSGTWLLL VPTGLEEHAA VHATVEALDA HGAAHEVLSI DGADARRDAI APLLSQLPDG
STPEGILSLL ALDRTPHPDH PAVPTGLAAT AALVQALGEA PLIAPLWCVT QGAVATNAAD
PLPHPHQAQI WGMGRVAALE HPRLWGGLID LPAVADARTP ARIAAMLVPG QPEDQVAIRS
TVLARRLEGA VCPDTAPTAW RPAGTTLITG GTGGLGAHVA RWLARQGAPH LHLVSRSGPD
APGAAQLAQD LDDLGATITI TACDVSDRAA LQRLLDTIPA EHPLTAVVHA AGLPENTPLA
ELDLPGIDAV LRPKALAAAL LHELTEELDL SAFVLFSSGA AAWGGGRQPS YAAANAYLDA
LAEHRRARGL AATSLAWAPW SDAGMASDEA AVDYYRRRGM RPLDTDLAIA SLQHALDHGD
TTITIADIDW ERFPAGFTAQ RPSPLLSDLA AAASPGPDAA PDATAAMSGS LQRQLATGTL
AQRHQLLLHH IQTHAAAILG HPTIDAIPPA QPFQELGFDS LTAVEFGHRL SAATGVDLPP
TLVFDHPTPK ELADYLRERL VEGEPTSEGH LLSELDRWDS VSEPSAVDEA ARRRITGRLR
LLLAKWNGTE RGTERSAAHS ELETATAEDI FDLISDEFGK SDEFGKS
//
