ID GFAP_BOVIN Reviewed; 428 AA.
AC Q28115; Q0P5L4; Q866S9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-APR-2013, entry version 89.
DE RecName: Full=Glial fibrillary acidic protein;
DE Short=GFAP;
GN Name=GFAP;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC Pecora; Bovidae; Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Subcommissural organ;
RX PubMed=10571109; DOI=10.1007/s004419900077;
RA Bouchard P., Ravet V., Meiniel R., Creveaux I., Meiniel A., Vellet A.,
RA Vigues B.;
RT "Use of a heterologous monoclonal antibody for cloning and detection
RT of glial fibrillary acidic protein in the bovine ventricular
RT ependyma.";
RL Cell Tissue Res. 298:207-216(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Thalamus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-416.
RA Guertler M., Alter T., Froeb A., Lange B., Johne R., Luecker E.,
RA Fehlhaber K.;
RT "Nucleotide sequence of the bovine glial fibrillary acidic protein
RT (GFAP) gene.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 197-227.
RX PubMed=8010535;
RA Kirkpatrick B.W., Hart G.L.;
RT "Conformation polymorphisms and targeted marker development.";
RL Anim. Genet. 25:77-82(1994).
CC -!- FUNCTION: GFAP, a class-III intermediate filament, is a cell-
CC specific marker that, during the development of the central
CC nervous system, distinguishes astrocytes from other glial cells.
CC -!- SUBUNIT: Interacts with SYNM (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Associated
CC with intermediate filaments (By similarity).
CC -!- PTM: Phosphorylated by PKN1 (By similarity).
CC -!- SIMILARITY: Belongs to the intermediate filament family.
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DR EMBL; Y08255; CAA69422.1; -; mRNA.
DR EMBL; BC119892; AAI19893.1; -; mRNA.
DR EMBL; AY174179; AAO18221.1; ALT_TERM; Genomic_DNA.
DR EMBL; L19867; AAA51413.1; -; Genomic_DNA.
DR IPI; IPI00716718; -.
DR PIR; PC2280; PC2280.
DR RefSeq; NP_776490.2; NM_174065.2.
DR UniGene; Bt.21127; -.
DR ProteinModelPortal; Q28115; -.
DR SMR; Q28115; 63-100, 290-368.
DR IntAct; Q28115; 1.
DR PaxDb; Q28115; -.
DR PRIDE; Q28115; -.
DR Ensembl; ENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534.
DR GeneID; 281189; -.
DR KEGG; bta:281189; -.
DR CTD; 2670; -.
DR eggNOG; NOG259463; -.
DR GeneTree; ENSGT00660000095344; -.
DR HOGENOM; HOG000230977; -.
DR HOVERGEN; HBG013015; -.
DR InParanoid; Q28115; -.
DR KO; K05640; -.
DR OMA; ASSNMQE; -.
DR OrthoDB; EOG48WC24; -.
DR NextBio; 20805246; -.
DR GO; GO:0044297; C:cell body; IEA:Compara.
DR GO; GO:0042995; C:cell projection; IEA:Compara.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005882; C:intermediate filament; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:Compara.
DR GO; GO:0005198; F:structural molecule activity; IEA:Compara.
DR GO; GO:0014002; P:astrocyte development; IEA:Compara.
DR GO; GO:0060020; P:Bergmann glial cell differentiation; IEA:Compara.
DR GO; GO:0030198; P:extracellular matrix organization; IEA:Compara.
DR GO; GO:0045109; P:intermediate filament organization; IEA:Compara.
DR GO; GO:0060291; P:long-term synaptic potentiation; IEA:Compara.
DR GO; GO:0010977; P:negative regulation of neuron projection development; IEA:Compara.
DR GO; GO:0031102; P:neuron projection regeneration; IEA:Compara.
DR GO; GO:0010625; P:positive regulation of Schwann cell proliferation; IEA:Compara.
DR GO; GO:0051580; P:regulation of neurotransmitter uptake; IEA:Compara.
DR InterPro; IPR016044; F.
DR InterPro; IPR001664; IF.
DR InterPro; IPR006821; Intermed_filament_DNA-bd.
DR InterPro; IPR018039; Intermediate_filament_CS.
DR PANTHER; PTHR23239; PTHR23239; 1.
DR Pfam; PF00038; Filament; 1.
DR Pfam; PF04732; Filament_head; 1.
DR PROSITE; PS00226; IF; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Complete proteome; Cytoplasm; Intermediate filament;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1 428 Glial fibrillary acidic protein.
FT /FTId=PRO_0000063804.
FT REGION 1 68 Head.
FT REGION 69 373 Rod.
FT REGION 69 100 Coil 1A.
FT REGION 101 111 Linker 1.
FT REGION 112 210 Coil 1B.
FT REGION 211 226 Linker 12.
FT REGION 227 248 Coil 2A.
FT REGION 249 252 Linker 2.
FT REGION 253 373 Coil 2B.
FT REGION 374 428 Tail.
FT MOD_RES 7 7 Phosphothreonine; by AURKB and ROCK1 (By
FT similarity).
FT MOD_RES 13 13 Phosphoserine; by AURKB and ROCK1 (By
FT similarity).
FT MOD_RES 34 34 Phosphoserine; by AURKB and ROCK1 (By
FT similarity).
FT MOD_RES 106 106 Phosphothreonine (By similarity).
FT MOD_RES 379 379 Phosphothreonine (By similarity).
FT CONFLICT 265 265 A -> R (in Ref. 1; CAA69422 and 3;
FT AAO18221).
FT CONFLICT 271 271 L -> V (in Ref. 1; CAA69422 and 3;
FT AAO18221).
FT CONFLICT 311 312 ER -> DA (in Ref. 1; CAA69422 and 3;
FT AAO18221).
FT CONFLICT 372 372 R -> Q (in Ref. 3; AAO18221).
FT CONFLICT 421 421 K -> P (in Ref. 1; CAA69422).
SQ SEQUENCE 428 AA; 49512 MW; 8DCCDF3E848BAD05 CRC64;
MERRRVTSAT RRSYVSSSEM VVGGRRLGPG TRLSLARMPP PLPARVDFSL AGALNSGFKE
TRASERAEMM ELNDRFASYI EKVRFLEQQN KALAAELNQL RAKEPTKLAD VYQAELRELR
LRLDQLTANS ARLEVERDNL AQDLGTLRQK LQDETNQRLE AENNLAAYRQ EADEATLARL
DLERKIESLE EEIRFLRKIH EEEVRELQEQ LAQQQVHVEM DVAKPDLTAA LREIRTQYEA
VASSNMHEAE EWYRSKFADL NDAAARNAEL LRQAKHEAND YRRQLQALTC DLESLRGTNE
SLERQMREQE ERHAREAASY QEALARLEEE GQSLKDEMAR HLQEYQDLLN VKLALDIEIA
TYRKLLEGEE NRITIPVQTF SNLQIRETSL DTKSVSEGHL KRNIVVKTVE MRDGEVIKES
KQEHKDVM
//
