ID Q28CD9_XENTR Unreviewed; 475 AA.
AC Q28CD9;
DT 04-APR-2006, integrated into UniProtKB/TrEMBL.
DT 04-APR-2006, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=Phosphoenolpyruvate carboxykinase, cytosolic [GTP] {ECO:0000256|ARBA:ARBA00040372};
DE EC=4.1.1.32 {ECO:0000256|ARBA:ARBA00012306};
DE AltName: Full=Serine-protein kinase PCK1 {ECO:0000256|ARBA:ARBA00042054};
DE Flags: Fragment;
GN Name=pck1 {ECO:0000313|EMBL:CAJ82933.1};
GN ORFNames=TTpA011c19.1-001 {ECO:0000313|EMBL:CAJ82933.1};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364 {ECO:0000313|EMBL:CAJ82933.1};
RN [1] {ECO:0000313|EMBL:CAJ82933.1}
RP NUCLEOTIDE SEQUENCE.
RA Amaya E., Ashurst J.L., Bonfield J.K., Croning M.D.R., Chen C-K.,
RA Davies R.M., Francis M.D., Garrett N., Gilchrist M.J., Grafham D.V.,
RA McLaren S.R., Papalopulu N., Rogers J., Smith J.C., Taylor R.G., Voigt J.,
RA Zorn A.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + L-seryl-[protein] = GDP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:64020, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:58189, ChEBI:CHEBI:83421;
CC Evidence={ECO:0000256|ARBA:ARBA00036764};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64021;
CC Evidence={ECO:0000256|ARBA:ARBA00036764};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:10388, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:37565, ChEBI:CHEBI:58189, ChEBI:CHEBI:58702; EC=4.1.1.32;
CC Evidence={ECO:0000256|ARBA:ARBA00035922};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10389;
CC Evidence={ECO:0000256|ARBA:ARBA00035922};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:10390;
CC Evidence={ECO:0000256|ARBA:ARBA00035922};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC {ECO:0000256|ARBA:ARBA00004742}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC family. {ECO:0000256|ARBA:ARBA00005796}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR926436; CAJ82933.1; -; mRNA.
DR AlphaFoldDB; Q28CD9; -.
DR UniPathway; UPA00138; -.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:UniProtKB-EC.
DR GO; GO:0043648; P:dicarboxylic acid metabolic process; IEA:UniProt.
DR GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00819; PEPCK_GTP; 1.
DR Gene3D; 3.90.228.20; -; 1.
DR Gene3D; 3.40.449.10; Phosphoenolpyruvate Carboxykinase, domain 1; 1.
DR Gene3D; 2.170.8.10; Phosphoenolpyruvate Carboxykinase, domain 2; 1.
DR HAMAP; MF_00452; PEPCK_GTP; 1.
DR InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008209; PEP_carboxykinase_GTP.
DR InterPro; IPR035077; PEP_carboxykinase_GTP_C.
DR InterPro; IPR035078; PEP_carboxykinase_GTP_N.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR PANTHER; PTHR11561; PHOSPHOENOLPYRUVATE CARBOXYKINASE; 1.
DR PANTHER; PTHR11561:SF19; PHOSPHOENOLPYRUVATE CARBOXYKINASE, CYTOSOLIC [GTP]; 1.
DR Pfam; PF00821; PEPCK_GTP; 1.
DR Pfam; PF17297; PEPCK_N; 1.
DR SUPFAM; SSF68923; PEP carboxykinase N-terminal domain; 1.
DR SUPFAM; SSF53795; PEP carboxykinase-like; 1.
DR PROSITE; PS00505; PEPCK_GTP; 1.
PE 2: Evidence at transcript level;
KW Decarboxylase {ECO:0000256|ARBA:ARBA00022793};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:CAJ82933.1};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Pyruvate {ECO:0000313|EMBL:CAJ82933.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..109
FT /note="Phosphoenolpyruvate carboxykinase GTP-utilising N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF17297"
FT DOMAIN 113..471
FT /note="Phosphoenolpyruvate carboxykinase C-terminal P-loop"
FT /evidence="ECO:0000259|Pfam:PF00821"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CAJ82933.1"
SQ SEQUENCE 475 AA; 52978 MW; B7B6A75FFA0457C5 CRC64;
GIGSPLSKIG IELTDSPYVV ASMRIMTRMG TAVLETLGDG EFVKCLHSVG CPLPLKKPLV
NNWPCNPEQT LIAHIPEQRE IISFGSGYGG NSLLGKKCFA LRIASRIAKE EGWLAEHMLI
LGITNPEGEK KYFAAAFPSA CGKTNLAMMK PSVPGWKIEC VGDDIAWMKF DEHGNLRAIN
PENGFFGVAP GTSAKTNPYA METIRNNTVF TNVGETSDGG IYWEGINESL DPGVTLTSWK
NKEWTPEDGE PCAHPNSRFC TPASQCPIID PEWESQEGVP IEGIIFGGRR PTGVPLVYEA
LNWQHGVFVG SAMRSEATAA AEHKGKVIMH DPFAMRPFFG YNFGKYLAHW LSMEHLPSTK
MPKIFHVNWF RKDKEGNFLW PGYGENIRVL EWMFKRINGE DCAKETPIGY IPAEGALNLK
GLGDVNVEEL FELSKEFWED EVKDIKKYLD DQVNADLPYE IERELSALEE RLKQL
//
