ID Q2HT46_MEDTR Unreviewed; 826 AA.
AC Q2HT46; A0A0C3UZD7; G7IM25;
DT 21-MAR-2006, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2006, sequence version 1.
DT 24-JAN-2024, entry version 93.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=11406418 {ECO:0000313|EnsemblPlants:AES64476};
GN OrderedLocusNames=MTR_2g025790 {ECO:0000313|EMBL:AES64476.2};
GN ORFNames=MtrDRAFT_AC150798g3v2 {ECO:0000313|EMBL:ABD32998.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880 {ECO:0000313|EMBL:ABD32998.1};
RN [1] {ECO:0000313|EMBL:ABD32998.1}
RP NUCLEOTIDE SEQUENCE.
RA Town C.D.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ABD32998.1}
RP NUCLEOTIDE SEQUENCE.
RG The International Medicago Genome Annotation Group;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AES64476.2, ECO:0000313|Proteomes:UP000002051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A17 {ECO:0000313|EMBL:AES64476.2}, and cv. Jemalong A17
RC {ECO:0000313|EnsemblPlants:AES64476,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E., Geurts R., Cannon S.B., Udvardi M.K.,
RA Benedito V.A., Mayer K.F., Gouzy J., Schoof H., Van de Peer Y., Proost S.,
RA Cook D.R., Meyers B.C., Spannagl M., Cheung F., De Mita S.,
RA Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K., Murray J.D.,
RA Naoumkina M.A., Rosen B., Silverstein K.A., Tang H., Rombauts S.,
RA Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M., Bellec A., Berger A.,
RA Berges H., Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA Deshpande S., Dai X., Doyle J.J., Dudez A.M., Farmer A.D., Fouteau S.,
RA Franken C., Gibelin C., Gish J., Goldstein S., Gonzalez A.J., Green P.J.,
RA Hallab A., Hartog M., Hua A., Humphray S.J., Jeong D.H., Jing Y.,
RA Jocker A., Kenton S.M., Kim D.J., Klee K., Lai H., Lang C., Lin S.,
RA Macmil S.L., Magdelenat G., Matthews L., McCorrison J., Monaghan E.L.,
RA Mun J.H., Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C., Sallet E.,
RA Samain S., Samson N., Sanders I., Saurat O., Scarpelli C., Schiex T.,
RA Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S., Singer S.R.,
RA Sinharoy S., Sterck L., Viollet A., Wang B.B., Wang K., Wang M., Wang X.,
RA Warfsmann J., Weissenbach J., White D.D., White J.D., Wiley G.B.,
RA Wincker P., Xing Y., Yang L., Yao Z., Ying F., Zhai J., Zhou L., Zuber A.,
RA Denarie J., Dixon R.A., May G.D., Schwartz D.C., Rogers J., Quetier F.,
RA Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [4] {ECO:0000313|EMBL:AES64476.2, ECO:0000313|Proteomes:UP000002051}
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES64476,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [5] {ECO:0000313|EnsemblPlants:AES64476}
RP IDENTIFICATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES64476};
RG EnsemblPlants;
RL Submitted (APR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; AC150798; ABD32998.1; -; Genomic_DNA.
DR EMBL; CM001218; AES64476.2; -; Genomic_DNA.
DR RefSeq; XP_003594225.2; XM_003594177.2.
DR AlphaFoldDB; Q2HT46; -.
DR STRING; 3880.Q2HT46; -.
DR PaxDb; 3880-AES64476; -.
DR EnsemblPlants; AES64476; AES64476; MTR_2g025790.
DR GeneID; 11406418; -.
DR Gramene; AES64476; AES64476; MTR_2g025790.
DR KEGG; mtr:11406418; -.
DR eggNOG; KOG0940; Eukaryota.
DR HOGENOM; CLU_002173_8_1_1; -.
DR OrthoDB; 948916at2759; -.
DR Proteomes; UP000002051; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR019956; Ubiquitin_dom.
DR PANTHER; PTHR11254:SF424; E3 UBIQUITIN-PROTEIN LIGASE UPL5; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR PRINTS; PR00348; UBIQUITIN.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000002051};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 45..120
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000259|PROSITE:PS50053"
FT DOMAIN 508..826
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 792
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 826 AA; 94458 MW; C531CC40E71A4659 CRC64;
MSSITKTIAG GCFHQQSING ATADHLSNHS PELKFNDTKT TSSELQFFVR MMWKCNTIVI
HASREDTVES ILQQISSKTK IPIEYQRLIY NGKQLQQQQS LSQCGIENDA NLQLVGQLRS
IGRSVVWNSA DDIVSMILNL CRGESLNGAS MIIHNHFAKY MNNFEYFYFF KLMKIPSLLV
ALYMSPFACN KNIADFSIES FIEICLDLKC KKLQGFYLEI LLDFCELLRG VGFTCDEPLY
VSCRDGFGNL LTLVGGVPIN NPNMKVLLRG VVDCVHEIAD ELLMYLDLSM NWDTAKGISY
KVVLDFVKFC GPLRMGFAEE QATSDESLNY DICYEEDPLF SGVPDQLHIV FIKLLSKMDE
CLQVMEDCLV NKEQGKGDVI HNGWSHYLII LKELFHISKF YSGAQEKFWG LLLHRKNVLP
HMIVRYVKKT DDHQWLLENK IVTDFESRRH LALMLFPDSK DEISGYEMLI DRSQVLAESF
EYMSRAKAKS LQGGIFMAFK NEKATGPGVL REWFVLVCRE IFNPKNALFV ACPNDHRRFF
PNAASKVNSL HLKYFIVSGR IIALALKKKV HVGIVFDRVF FKQLAGNYII TLEDIRDADP
IMYSSCKQIL EMDADYIDSD ALGLTFSTEV EELGHRELIE LCPGGESLVV DSKNREKYVH
LLIQNRFVTS ISKQVSHFAE GFADILSCSR LEFFQFLDLE DFDLMLHGSE NAISVEDWKV
HTKYHGYKEN DHQISWFWKI VGRMSAEQKK VLLFFWTSVK HLPVEGFRGL SSTLLISKSS
KPDNHLPSSH TCFYKLCFPP YSSMAIMQDR LGIITQEHIS CSFGTA
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