ID RNC_AYWBP Reviewed; 237 AA.
AC Q2NJY3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 01-MAY-2013, entry version 48.
DE RecName: Full=Ribonuclease 3;
DE EC=3.1.26.3;
DE AltName: Full=Ribonuclease III;
DE Short=RNase III;
GN Name=rnc; OrderedLocusNames=AYWB_143;
OS Aster yellows witches'-broom phytoplasma (strain AYWB).
OC Bacteria; Tenericutes; Mollicutes; Acholeplasmatales;
OC Acholeplasmataceae; Candidatus Phytoplasma;
OC Candidatus Phytoplasma asteris.
OX NCBI_TaxID=322098;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AYWB;
RX PubMed=16672622; DOI=10.1128/JB.188.10.3682-3696.2006;
RA Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A.,
RA Shevchenko D.V., Tsukerman K., Walunas T., Lapidus A., Campbell J.W.,
RA Hogenhout S.A.;
RT "Living with genome instability: the adaptation of phytoplasmas to
RT diverse environments of their insect and plant hosts.";
RL J. Bacteriol. 188:3682-3696(2006).
CC -!- FUNCTION: Digests double-stranded RNA. Involved in the processing
CC of primary rRNA transcript to yield the immediate precursors to
CC the large and small rRNAs (23S and 16S). Also processes some
CC mRNAs, and tRNAs when they are encoded in the rRNA operon (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
CC phosphomonoester.
CC -!- COFACTOR: Mg(2+) (By similarity).
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
CC -!- SIMILARITY: Contains 1 RNase III domain.
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DR EMBL; CP000061; ABC65260.1; -; Genomic_DNA.
DR RefSeq; YP_456339.1; NC_007716.1.
DR HSSP; Q9X0I6; 1O0W.
DR ProteinModelPortal; Q2NJY3; -.
DR STRING; 322098.AYWB_143; -.
DR EnsemblBacteria; ABC65260; ABC65260; AYWB_143.
DR GeneID; 3865836; -.
DR KEGG; ayw:AYWB_143; -.
DR PATRIC; 21004222; VBIAstYel136969_0168.
DR eggNOG; COG0571; -.
DR HOGENOM; HOG000246808; -.
DR KO; K03685; -.
DR OMA; LTHKSCK; -.
DR ProtClustDB; CLSK343805; -.
DR BioCyc; CPHY322098:GJ7E-150-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:HAMAP.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0016075; P:rRNA catabolic process; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1520.10; -; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR HAMAP; MF_00104; RNase_III; 1; -.
DR InterPro; IPR001159; Ds-RNA-bd.
DR InterPro; IPR014720; dsRNA-bd-like_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR PANTHER; PTHR11207; PTHR11207; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 1.
DR SUPFAM; SSF69065; RNase_III; 1.
DR TIGRFAMs; TIGR02191; RNaseIII; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 1.
DR PROSITE; PS50142; RNASE_3_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW Metal-binding; mRNA processing; Nuclease; RNA-binding;
KW rRNA processing; rRNA-binding; tRNA processing.
FT CHAIN 1 237 Ribonuclease 3.
FT /FTId=PRO_1000075721.
FT DOMAIN 4 130 RNase III.
FT DOMAIN 154 222 DRBM.
FT ACT_SITE 49 49 Potential.
FT ACT_SITE 119 119 By similarity.
FT METAL 45 45 Magnesium (By similarity).
FT METAL 116 116 Magnesium (By similarity).
FT METAL 119 119 Magnesium (By similarity).
SQ SEQUENCE 237 AA; 26797 MW; 9B65C152553E394B CRC64;
MKNIQILFQT LNIFPKNLVL YKQALTHSSY SNEQTPPQED NERLEFLGDA IVGLLMADYL
YSQSKEDEGI MTKKRAQAVC EKSLTIYAQK IELQNYLLLG KGEKNKDFNA KGIMADTFEA
LFGAIYLDLG YLTAKKVFHN IVLPHLAKTI YIIDFKTQLQ EIVQSEKKTI QYKIVQEQGP
AHSKNFVAEV YLEKNLLGTG EGSTKKSAEQ KAAQQALSKV AKTKDLIKNK GVKEKEL
//
