ID Q2TDH2_9HYME Unreviewed; 277 AA.
AC Q2TDH2;
DT 24-JAN-2006, integrated into UniProtKB/TrEMBL.
DT 24-JAN-2006, sequence version 1.
DT 24-JAN-2024, entry version 51.
DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE Flags: Fragment;
OS Megachile pugnata.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea;
OC Anthophila; Megachilidae; Megachilinae; Megachile.
OX NCBI_TaxID=156341 {ECO:0000313|EMBL:AAY21444.1};
RN [1] {ECO:0000313|EMBL:AAY21444.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=16412668; DOI=10.1016/j.ympev.2005.09.022;
RA Danforth B.N., Fang J., Sipes S.;
RT "Analysis of family-level relationships in bees (Hymenoptera: Apiformes)
RT using 28S and two previously unexplored nuclear genes: CAD and RNA
RT polymerase II.";
RL Mol. Phylogenet. Evol. 39:358-372(2006).
RN [2] {ECO:0000313|EMBL:AAY21444.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=17015826; DOI=10.1073/pnas.0604033103;
RA Danforth B.N., Sipes S., Fang J., Brady S.G.;
RT "The history of early bee diversification based on five genes plus
RT morphology.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15118-15123(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460}.
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DR EMBL; AY945143; AAY21444.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2TDH2; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..150
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04983"
FT DOMAIN 177..277
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF05000"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAY21444.1"
FT NON_TER 277
FT /evidence="ECO:0000313|EMBL:AAY21444.1"
SQ SEQUENCE 277 AA; 31013 MW; DFAA91D0C10A1925 CRC64;
TAVRKMTKRD VFIEKEQMMN ILMFLPSWDG KMPQPCILKP KPLWTGKQIF SLIIPGNVNM
IRTHSTHPDE EDDGPYKWIS PGDTKVMVEH GELVMGILCK KTLGTSAGSL LHICMLELGH
EVCGRFYGNI QTVINNWLLL EGHSIGIGDT IADPQTYLEI QKAIKKAKED VIEVIQKAHN
MELEPTPGNT LRQTFENQVN RILNDARDKT GGSAKKSLTE YNNLKAMVVS GSKGSNINIS
QVIACVGQQN VEGKRIPFGF RKRTLPHFIK DDYGPES
//
