ID AGALD_ASPOR Reviewed; 657 AA.
AC Q2UI87;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 2.
DT 01-MAY-2013, entry version 37.
DE RecName: Full=Probable alpha-galactosidase D;
DE EC=3.2.1.22;
DE AltName: Full=Melibiase D;
DE Flags: Precursor;
GN Name=aglD; ORFNames=AO090023000151;
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Trichocomaceae;
OC mitosporic Trichocomaceae; Aspergillus.
OX NCBI_TaxID=510516;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G.,
RA Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K.,
RA Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W.,
RA Galagan J.E., Nierman W.C., Yu J., Archer D.B., Bennett J.W.,
RA Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H.,
RA Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R.,
RA Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N.,
RA Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I.,
RA Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y.,
RA Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y.,
RA Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K.,
RA Kuhara S., Ogasawara N., Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
CC -!- FUNCTION: Hydrolyzes a variety of simple alpha-D-galactoside as
CC well as more complex molecules such as oligosaccharides and
CC polysaccharides (By similarity).
CC -!- CATALYTIC ACTIVITY: Hydrolysis of terminal, non-reducing alpha-D-
CC galactose residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.
CC -!- SUBCELLULAR LOCATION: Secreted (By similarity).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 27 family.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE58728.1; Type=Erroneous gene model prediction;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AP007157; BAE58728.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001820730.2; XM_001820678.2.
DR EnsemblFungi; CADAORAT00007299; CADAORAP00007154; CADAORAG00007299.
DR GeneID; 5992732; -.
DR KEGG; aor:AOR_1_260144; -.
DR eggNOG; NOG68897; -.
DR HOGENOM; HOG000161224; -.
DR OrthoDB; EOG41CB4R; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0052692; F:raffinose alpha-galactosidase activity; IEA:EC.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.1180; -; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR013780; Glyco_hydro_13_b.
DR InterPro; IPR002241; Glyco_hydro_27.
DR InterPro; IPR000111; Glyco_hydro_GHD.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF02065; Melibiase; 1.
DR PRINTS; PR00740; GLHYDRLASE27.
DR SUPFAM; SSF51445; Glyco_hydro_cat; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; FALSE_NEG.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Complete proteome; Disulfide bond;
KW Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Secreted; Signal.
FT SIGNAL 1 16 Potential.
FT CHAIN 17 657 Probable alpha-galactosidase D.
FT /FTId=PRO_0000395073.
FT REGION 200 204 Substrate binding (By similarity).
FT ACT_SITE 155 155 Nucleophile (By similarity).
FT ACT_SITE 222 222 Proton donor (By similarity).
FT CARBOHYD 47 47 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 91 91 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 182 182 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 191 191 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 291 291 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 438 438 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 460 460 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 505 505 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 539 539 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 543 543 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 582 582 N-linked (GlcNAc...) (Potential).
FT DISULFID 124 157 By similarity.
SQ SEQUENCE 657 AA; 71140 MW; 50917D2103A5E8DF CRC64;
MLPKIFYLSL LPAALGHPHL QPRLDNGLAR TPQMGWNTYN HYSCSPNETI VRSNAQALVD
LGLASLGYRY VTTDCGWTVA DRLSDGSLTW NETLFPKGFP ALGKYLHDLD LLFGVYQDSG
IKLCGSPPDN VGSLYHEDQD ARTFASWEVD SLKYDNCYSD AATGYPNVNY EPSTSPQPRF
ANMSRALAAQ NRSMVFQVCE WGIDFPARWA PALGHSWRIG NDIIPHWRAI YRTLNQAVPQ
TSFAGPGQWP DLDMLFVGND ILSIPEEQTH FSLWAILKSP LTIGAALKDD NTSINDESLQ
ILKQEEVIGY NQDSLGVSAS LRRRWTEEGY EVWSGPLSGG RTVAALINWK DEARELTLDL
PDIGLQFAGT VKNIWDGTTA QNVKTSYTAK VQSHGTILLE LQDTTASGQY PTDTFATSTD
SSTTFESIYG VTTSFRYNIT VKLSEASSSG DVNIQSTASN KTITAQVSAS GTEASAQIPL
LAGSSNSITI VSPQSVDAIT ITPPNGTYFP NTAFTTTGDA DTVSCGAGYC QPVGSKIGNI
STNGTARAVI PATAGTKYLA IDYINNDVAF DSAWDWGSNS RNLTVSVNGN KPVRIEVPLS
GQHSELFGPG KGWWDTATIG VLTEGWKDGD NDVVIGNEGG ESGFTSYGPD FVGLRVL
//
