ID RSMB_SHIDS Reviewed; 429 AA.
AC Q32B61;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 01-MAY-2013, entry version 54.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase B;
DE EC=2.1.1.176;
DE AltName: Full=16S rRNA m5C967 methyltransferase;
DE AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RsmB;
GN Name=rsmB; Synonyms=sun; OrderedLocusNames=SDY_3465;
OS Shigella dysenteriae serotype 1 (strain Sd197).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=300267;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sd197;
RX PubMed=16275786; DOI=10.1093/nar/gki954;
RA Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X.,
RA Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S.,
RA Nie H., Peng J., Xu J., Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y.,
RA Qiang B., Hou Y., Yu J., Jin Q.;
RT "Genome dynamics and diversity of Shigella species, the etiologic
RT agents of bacillary dysentery.";
RL Nucleic Acids Res. 33:6445-6458(2005).
CC -!- FUNCTION: Specifically methylates the cytosine at position 967
CC (m5C967) of 16S rRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + cytosine(967) in 16S
CC rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S
CC rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmB/NOP
CC family.
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DR EMBL; CP000034; ABB63444.1; -; Genomic_DNA.
DR RefSeq; YP_404935.1; NC_007606.1.
DR HSSP; P36929; 1SQF.
DR ProteinModelPortal; Q32B61; -.
DR SMR; Q32B61; 5-428.
DR STRING; 300267.SDY_3465; -.
DR PRIDE; Q32B61; -.
DR EnsemblBacteria; ABB63444; ABB63444; SDY_3465.
DR GeneID; 3798715; -.
DR KEGG; sdy:SDY_3465; -.
DR PATRIC; 18697459; VBIShiDys99784_4118.
DR eggNOG; COG0144; -.
DR HOGENOM; HOG000037300; -.
DR KO; K03500; -.
DR OMA; KPPMWLR; -.
DR ProtClustDB; PRK10901; -.
DR BioCyc; SDYS300267:GJEW-3461-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016434; F:rRNA (cytosine) methyltransferase activity; IEA:HAMAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
DR Gene3D; 1.10.940.10; -; 1.
DR HAMAP; MF_01856; 16SrRNA_methyltr_B; 1; -.
DR InterPro; IPR001678; Fmu/NOL1/Nop2p.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR004573; rRNA_ssu_MeTfrase_B.
DR InterPro; IPR023541; rRNA_ssu_MeTfrase_B_ent.
DR Pfam; PF01189; Nol1_Nop2_Fmu; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB_RsmB_TIM44; 1.
DR TIGRFAMs; TIGR00563; rsmB; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase; Reference proteome;
KW RNA-binding; rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 429 Ribosomal RNA small subunit
FT methyltransferase B.
FT /FTId=PRO_0000366178.
FT REGION 254 260 S-adenosyl-L-methionine binding (By
FT similarity).
FT ACT_SITE 375 375 Nucleophile (Potential).
FT BINDING 277 277 S-adenosyl-L-methionine (By similarity).
FT BINDING 303 303 S-adenosyl-L-methionine (By similarity).
FT BINDING 322 322 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 429 AA; 48349 MW; 9B84CC085A4F972A CRC64;
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG VLRTLSQLDW
LINKLMARPM SGKQRTVHYL IMVGLYQLLY TRIPPHAALA ETVEGAIAIK RPQLKGLING
VLRQFQRQQE ELLAEFNTSD ARYLHPSWLL KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR
THHSRDSWLA LLDEAGMKGF PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT
WLAPQNGKHI LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA QLQSEILDAI
WPHLKSGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE TGTPEQPGKQ NLPGAEEGDG
FFYAKLIKK
//
