ID FDFT_BOVIN Reviewed; 417 AA.
AC Q32KR6;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 29-MAY-2013, entry version 50.
DE RecName: Full=Squalene synthase;
DE Short=SQS;
DE Short=SS;
DE EC=2.5.1.21;
DE AltName: Full=FPP:FPP farnesyltransferase;
DE AltName: Full=Farnesyl-diphosphate farnesyltransferase;
GN Name=FDFT1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC Pecora; Bovidae; Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Critical branch point enzyme of isoprenoid biosynthesis
CC that is thought to regulate the flux of isoprene intermediates
CC through the sterol pathway (By similarity).
CC -!- CATALYTIC ACTIVITY: 2 farnesyl diphosphate + NAD(P)H = squalene +
CC 2 diphosphate + NAD(P)(+).
CC -!- COFACTOR: Magnesium (By similarity).
CC -!- PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol
CC from farnesyl diphosphate: step 1/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein (By similarity).
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
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DR EMBL; BC109959; AAI09960.1; -; mRNA.
DR IPI; IPI01028010; -.
DR UniGene; Bt.14255; -.
DR ProteinModelPortal; Q32KR6; -.
DR SMR; Q32KR6; 38-370.
DR STRING; 9913.ENSBTAP00000016504; -.
DR PRIDE; Q32KR6; -.
DR eggNOG; COG1562; -.
DR HOGENOM; HOG000186940; -.
DR HOVERGEN; HBG002370; -.
DR InParanoid; Q32KR6; -.
DR OrthoDB; EOG4QVCC2; -.
DR BioCyc; CATTLE:281767-MONOMER; -.
DR UniPathway; UPA00767; UER00751.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0004310; F:farnesyl-diphosphate farnesyltransferase activity; IEA:EC.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0051996; F:squalene synthase activity; IEA:EC.
DR GO; GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR006449; Squal_synth.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR008949; Terpenoid_synth.
DR Pfam; PF00494; SQS_PSY; 1.
DR SUPFAM; SSF48576; Terpenoid_synth; 1.
DR TIGRFAMs; TIGR01559; squal_synth; 1.
DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 2: Evidence at transcript level;
KW Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome;
KW Endoplasmic reticulum; Isoprene biosynthesis; Lipid biosynthesis;
KW Lipid metabolism; Magnesium; Membrane; Multifunctional enzyme; NADP;
KW Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1 417 Squalene synthase.
FT /FTId=PRO_0000290005.
FT TRANSMEM 284 304 Helical; (Potential).
FT TRANSMEM 384 404 Helical; (Potential).
SQ SEQUENCE 417 AA; 48304 MW; C6A2FD8EA5C85BC7 CRC64;
MEFVKCLGHP EEFYNLLRFQ MGGRRKVIPK MDQDSLSSSL KTCYKYLNQT SRSFAAVIQA
LDGEMRHAVC IFYLVLRALD TLEDDMTISI ERKVPLLHNF HSYLYEPDWR FTESKEKDRQ
VLEDFPTISL EFRNLAEKYQ TVIVDVCQKM GFGMAEFLDK RVTSEREWDK YCHYVAGLVG
IGLSRLFPAS ELEDPLIGED TERANSMGLF LQKTNIIRDY LEDQREGREF WPQETWSKYV
KKLGDFAKPE NIDLAVQCLN ELITNTLHHI PDVITYLSRL RNQSIFNFCA IPQVMAIATL
AACYNNQQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIHQ YMEEIYHRIP NSDPCSTKTQ
QIISTIRTQN LPNCQLVSRS HYSPIYLSFV MLLAALSWQY LSTLSQVTED YVQTGEH
//
