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Database: UniProt
Entry: Q32LQ4
LinkDB: Q32LQ4
Original site: Q32LQ4 
ID   BHMT1_DANRE             Reviewed;         400 AA.
AC   Q32LQ4; Q5PSM1;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   16-APR-2014, entry version 65.
DE   RecName: Full=Betaine--homocysteine S-methyltransferase 1;
DE            EC=2.1.1.5;
GN   Name=bhmt; ORFNames=wu:fb53h01, zgc:123027;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Lapek J.D. Jr., Warren J.T. Jr.;
RT   "Molecular genetic analysis of folate metabolism in the zebrafish.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the regulation of homocysteine metabolism.
CC       Converts betaine and homocysteine to dimethylglycine and
CC       methionine, respectively. This reaction is also required for the
CC       irreversible oxidation of choline (By similarity).
CC   -!- CATALYTIC ACTIVITY: Trimethylammonioacetate + L-homocysteine =
CC       dimethylglycine + L-methionine.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Amine and polyamine degradation; betaine degradation;
CC       sarcosine from betaine: step 1/2.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de
CC       novo pathway; L-methionine from L-homocysteine (BhmT route): step
CC       1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Contains 1 Hcy-binding domain.
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DR   EMBL; AY830415; AAV74219.1; -; mRNA.
DR   EMBL; BC109472; AAI09473.1; -; mRNA.
DR   RefSeq; NP_001012498.1; NM_001012480.1.
DR   UniGene; Dr.75610; -.
DR   ProteinModelPortal; Q32LQ4; -.
DR   SMR; Q32LQ4; 7-393.
DR   STRING; 7955.ENSDARP00000040421; -.
DR   GeneID; 322228; -.
DR   KEGG; dre:322228; -.
DR   CTD; 635; -.
DR   ZFIN; ZDB-GENE-030131-947; bhmt.
DR   eggNOG; COG0646; -.
DR   HOGENOM; HOG000231636; -.
DR   HOVERGEN; HBG080367; -.
DR   KO; K00544; -.
DR   PhylomeDB; Q32LQ4; -.
DR   UniPathway; UPA00051; UER00083.
DR   UniPathway; UPA00291; UER00432.
DR   NextBio; 20807724; -.
DR   PRO; PR:Q32LQ4; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047150; F:betaine-homocysteine S-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008898; F:homocysteine S-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006579; P:amino-acid betaine catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0055123; P:digestive system development; IMP:ZFIN.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:InterPro.
DR   GO; GO:0003323; P:type B pancreatic cell development; IMP:ZFIN.
DR   Gene3D; 3.20.20.330; -; 1.
DR   InterPro; IPR017226; Betaine-hCys_S-MeTrfase_BHMT.
DR   InterPro; IPR003726; S_MeTrfase.
DR   Pfam; PF02574; S-methyl_trans; 1.
DR   PIRSF; PIRSF037505; Betaine_HMT; 1.
DR   SUPFAM; SSF82282; SSF82282; 1.
DR   PROSITE; PS50970; HCY; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Cytoplasm; Metal-binding; Methyltransferase;
KW   Reference proteome; Transferase; Zinc.
FT   CHAIN         1    400       Betaine--homocysteine S-methyltransferase
FT                                1.
FT                                /FTId=PRO_0000273221.
FT   DOMAIN        8    309       Hcy-binding.
FT   METAL       212    212       Zinc (By similarity).
FT   METAL       294    294       Zinc (By similarity).
FT   METAL       295    295       Zinc (By similarity).
FT   CONFLICT    189    189       H -> P (in Ref. 1; AAV74219).
FT   CONFLICT    192    192       I -> T (in Ref. 1; AAV74219).
FT   CONFLICT    224    224       A -> V (in Ref. 1; AAV74219).
SQ   SEQUENCE   400 AA;  44066 MW;  A6A3A9A824D5D26A CRC64;
     MAPVGSKRGV LERLNAGEVV IGDGGFVFAL EKRGYVKAGP WTPEAAAEHP EAVRQLHREF
     LRAGSNVMQT FTFYASDDKL ENRGNKLSFT GQQINEAACD LAREVANEGD ALVAGGVSQT
     PSYLSCKSEE EVKKTFKKQL DVFIKKNVDL LIAEYFEHVE EAEWAVQVLK ATGKPVAATL
     CIGPDGDMHG VIPGECAVRL VKAGADIVGV NCHFDPLTCV KTVAMMKAAV EKAGLKAHYM
     TQPLAYHTPD CSCQGFIDLP EFPFALEPRI LTRWEMQQYA REAYKAGIRY IGGCCGFEPY
     HIRAVAEELS AERGFLPEAS QKHGLWGSGL EMHTKPWVRA RARRDYWEKL KPASGRPLCP
     SMSTPDGWGV TRGHAALMQQ KEATTAEQLR PLFQQADAKH
//
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