ID SYFB_BURP1 Reviewed; 810 AA.
AC Q3JT07;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 01-MAY-2013, entry version 54.
DE RecName: Full=Phenylalanine--tRNA ligase beta subunit;
DE EC=6.1.1.20;
DE AltName: Full=Phenylalanyl-tRNA synthetase beta subunit;
DE Short=PheRS;
GN Name=pheT; OrderedLocusNames=BURPS1710b_1896;
OS Burkholderia pseudomallei (strain 1710b).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; pseudomallei group.
OX NCBI_TaxID=320372;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1710b;
RA Woods D.E., Nierman W.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP +
CC diphosphate + L-phenylalanyl-tRNA(Phe).
CC -!- COFACTOR: Binds 2 magnesium ions per tetramer (By similarity).
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the phenylalanyl-tRNA synthetase beta
CC subunit family. Type 1 subfamily.
CC -!- SIMILARITY: Contains 1 B5 domain.
CC -!- SIMILARITY: Contains 1 FDX-ACB domain.
CC -!- SIMILARITY: Contains 1 tRNA-binding domain.
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DR EMBL; CP000124; ABA48741.1; -; Genomic_DNA.
DR RefSeq; YP_333296.1; NC_007434.1.
DR ProteinModelPortal; Q3JT07; -.
DR STRING; 320372.BURPS1710b_1896; -.
DR EnsemblBacteria; ABA48741; ABA48741; BURPS1710b_1896.
DR GeneID; 3689438; -.
DR KEGG; bpm:BURPS1710b_1896; -.
DR PATRIC; 19236198; VBIBurPse115837_1977.
DR eggNOG; COG0072; -.
DR HOGENOM; HOG000292085; -.
DR KO; K01890; -.
DR OMA; MKFSEQW; -.
DR ProtClustDB; PRK00629; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:HAMAP.
DR GO; GO:0000049; F:tRNA binding; IEA:HAMAP.
DR GO; GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:InterPro.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.56.20; -; 1.
DR Gene3D; 3.30.70.380; -; 1.
DR Gene3D; 3.50.40.10; -; 1.
DR HAMAP; MF_00283; Phe_tRNA_synth_beta1; 1; -.
DR InterPro; IPR005146; B3/B4_tRNA-bd.
DR InterPro; IPR009061; DNA-bd_dom_put.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004532; Phe-tRNA-ligase_IIc_bsu_bac.
DR InterPro; IPR020825; Phe-tRNA_synthase_B3/B4.
DR InterPro; IPR005121; PheS_beta_Fdx_antiC-bd.
DR InterPro; IPR002547; tRNA-bd_dom.
DR InterPro; IPR005147; tRNA_synthase_B5-dom.
DR Pfam; PF03483; B3_4; 1.
DR Pfam; PF03484; B5; 1.
DR Pfam; PF03147; FDX-ACB; 1.
DR Pfam; PF01588; tRNA_bind; 1.
DR SMART; SM00873; B3_4; 1.
DR SMART; SM00874; B5; 1.
DR SMART; SM00896; FDX-ACB; 1.
DR SUPFAM; SSF56037; B3_4; 1.
DR SUPFAM; SSF54991; Fdx_AntiC_bd; 1.
DR SUPFAM; SSF50249; Nucleic_acid_OB; 1.
DR SUPFAM; SSF46955; Putativ_DNA_bind; 1.
DR TIGRFAMs; TIGR00472; pheT_bact; 1.
DR PROSITE; PS51483; B5; 1.
DR PROSITE; PS51447; FDX_ACB; 1.
DR PROSITE; PS50886; TRBD; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW Ligase; Magnesium; Metal-binding; Nucleotide-binding;
KW Protein biosynthesis; RNA-binding; tRNA-binding.
FT CHAIN 1 810 Phenylalanine--tRNA ligase beta subunit.
FT /FTId=PRO_0000232049.
FT DOMAIN 39 154 tRNA-binding.
FT DOMAIN 405 480 B5.
FT DOMAIN 707 809 FDX-ACB.
FT METAL 458 458 Magnesium (By similarity).
FT METAL 464 464 Magnesium; via carbonyl oxygen (By
FT similarity).
FT METAL 467 467 Magnesium (By similarity).
FT METAL 468 468 Magnesium (By similarity).
SQ SEQUENCE 810 AA; 88647 MW; 34B6F7DA6389542C CRC64;
MQFPESWLRT FVDPQLTTDE LSHALTMAGL EVESLRPAAP PTEKIVVGRV LEVVKHPDAD
KLNVCQVDAG TGATLQIVCG APNVAPGIKV PVALVGAKLP PAEEGGAPFA IKLSKLRGVE
SQGMLCSARE LKLSEDHSGL MILPEGTPVG QDIREALNLD DTVFEIKLTP NKADCLSVFG
IARETAAITG APLAAPDIRP VLAELTETLP VKISAPDLCG RFSGRVIRGV NARAKTPHWM
VERLERAGQR SVSALVDISN YVMFELGRPS HVFDLDKIHG GIDVRWGKRG ESLKLLNGNT
IELDETVGVI SDGAQVESLA GIMGGDSTAV TLDTTNIYLE AAFWWPDSIR GRARKYNFST
DAAHRFERGV DYSTTVEHVE RITQLILDIC GGQAGPVDDQ IVNLPQRAPV SMRASRANRI
IGVEIGEDEI AQIFTRLGLA FERDGDVFRV TPPPHRFDIE IEEDLIEEVA RIYGFEKIPA
RPPVAKSEMR ATDETRRSVH AIRHALAARD YAETVNFSFV DAEWERDFAG NDNPVRLLNP
IASQLSVMRT TLFGSLVGVL RHNLNRRAER VRVFEAGRVF VADPSVKAGE LAVEGYAQPK
RIGALAYGPV VEEQWGTATR QVDYFDVKGD LEALLAPVPA RFVKAEHPAL HPGRSARIEV
DGHAVGWIGE LHPRLMQKYE LPHAPVMFEI DTDALVARAL PAPSEVSKFP PVRRDIAVVV
DQKVEVQALF DEMKKALADD ACKFVQRVAL FDEFRAKSNT SGGLSAHEKS LAFRVTLQDA
AGTLQDETVD QAIQTLVDRM ARVYGARLRG
//
