GenomeNet

Database: UniProt
Entry: Q3K7J8
LinkDB: Q3K7J8
Original site: Q3K7J8 
ID   PYRC_PSEPF              Reviewed;         348 AA.
AC   Q3K7J8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   14-MAY-2014, entry version 61.
DE   RecName: Full=Dihydroorotase;
DE            Short=DHOase;
DE            EC=3.5.2.3;
GN   Name=pyrC; OrderedLocusNames=Pfl01_4519;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 1 subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000094; ABA76256.1; -; Genomic_DNA.
DR   RefSeq; YP_350247.1; NC_007492.2.
DR   ProteinModelPortal; Q3K7J8; -.
DR   SMR; Q3K7J8; 3-343.
DR   STRING; 205922.Pfl01_4519; -.
DR   PRIDE; Q3K7J8; -.
DR   EnsemblBacteria; ABA76256; ABA76256; Pfl01_4519.
DR   GeneID; 3717888; -.
DR   KEGG; pfo:Pfl01_4519; -.
DR   PATRIC; 19891196; VBIPseFlu44242_4525.
DR   eggNOG; COG0418; -.
DR   HOGENOM; HOG000256259; -.
DR   KO; K01465; -.
DR   OMA; LKRNVHQ; -.
DR   OrthoDB; EOG6TFCMH; -.
DR   BioCyc; PFLU205922:GJBD-4585-MONOMER; -.
DR   UniPathway; UPA00070; UER00117.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_00219; PyrC_type1; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004721; DHOdimr.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   PIRSF; PIRSF001237; DHOdimr; 1.
DR   TIGRFAMs; TIGR00856; pyrC_dimer; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    348       Dihydroorotase.
FT                                /FTId=PRO_1000024038.
FT   METAL        14     14       Zinc 1 (By similarity).
FT   METAL        16     16       Zinc 1 (By similarity).
FT   METAL       100    100       Zinc 1; via carbamate group (By
FT                                similarity).
FT   METAL       100    100       Zinc 2; via carbamate group (By
FT                                similarity).
FT   METAL       137    137       Zinc 2 (By similarity).
FT   METAL       175    175       Zinc 2 (By similarity).
FT   METAL       248    248       Zinc 1 (By similarity).
FT   MOD_RES     100    100       N6-carboxylysine (By similarity).
SQ   SEQUENCE   348 AA;  38480 MW;  AB078C90E2570A7E CRC64;
     MSDRLTLLRP DDWHIHLRDG AVLTNTVADV ARTFGRAIIM PNLVPPVRNA AEADGYRQRI
     LAARPAGSRF EPLMVLYLTD RTQPEEIREA KASGFVHAAK LYPAGATTNS DSGVTSIDKI
     FPVLEAMAEA GMPLLIHGEV TRGDVDVFDR EKIFIDEHMR RVVERFPTLK VVFEHITTGD
     AVQFVNEASA NVGATITAHH LLYNRNHMLV GGIRPHFYCL PILKRNTHQE ALLDAATSGS
     AKFFLGTDSA PHAQHAKEAA CGCAGCYTAY AAIELYAEAF EQRNALDKLE AFASLNGPRF
     YGLPANTDRI TLVRDEWTAP TSLPFGELTV IPLRAGEKLR WRLLEEHA
//
DBGET integrated database retrieval system