ID Q3SGY7_THIDA Unreviewed; 763 AA.
AC Q3SGY7;
DT 11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT 11-OCT-2005, sequence version 1.
DT 27-MAR-2024, entry version 116.
DE SubName: Full=Elongator protein 3/MiaB/NifB {ECO:0000313|EMBL:AAZ98106.1};
GN OrderedLocusNames=Tbd_2153 {ECO:0000313|EMBL:AAZ98106.1};
OS Thiobacillus denitrificans (strain ATCC 25259).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Thiobacillaceae; Thiobacillus.
OX NCBI_TaxID=292415 {ECO:0000313|EMBL:AAZ98106.1, ECO:0000313|Proteomes:UP000008291};
RN [1] {ECO:0000313|EMBL:AAZ98106.1, ECO:0000313|Proteomes:UP000008291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25259 {ECO:0000313|EMBL:AAZ98106.1,
RC ECO:0000313|Proteomes:UP000008291};
RX PubMed=16452431; DOI=10.1128/JB.188.4.1473-1488.2006;
RA Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W.,
RA Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.;
RT "The genome sequence of the obligately chemolithoautotrophic, facultatively
RT anaerobic bacterium Thiobacillus denitrificans.";
RL J. Bacteriol. 188:1473-1488(2006).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01251};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|HAMAP-Rule:MF_01251};
CC -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000256|HAMAP-
CC Rule:MF_01251}.
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DR EMBL; CP000116; AAZ98106.1; -; Genomic_DNA.
DR RefSeq; WP_011312665.1; NC_007404.1.
DR AlphaFoldDB; Q3SGY7; -.
DR STRING; 292415.Tbd_2153; -.
DR KEGG; tbd:Tbd_2153; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_018288_2_0_4; -.
DR OrthoDB; 9803479at2; -.
DR Proteomes; UP000008291; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR HAMAP; MF_01251; UPF0313; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR019824; Leghaemoglobin_Fe_BS.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR024560; UPF0313_C.
DR InterPro; IPR013704; UPF0313_N.
DR NCBIfam; TIGR03904; SAM_YgiQ; 1.
DR PANTHER; PTHR32331; UPF0313 PROTEIN YGIQ; 1.
DR PANTHER; PTHR32331:SF0; UPF0313 PROTEIN YGIQ; 1.
DR Pfam; PF11842; DUF3362; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS00208; PLANT_GLOBIN; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP-
KW Rule:MF_01251};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01251}; Reference proteome {ECO:0000313|Proteomes:UP000008291};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01251}.
FT DOMAIN 374..641
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 680..763
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 388
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 392
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 395
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
SQ SEQUENCE 763 AA; 84248 MW; 1A058D1F65B98B74 CRC64;
MTNALFSHRK HWAARFGTAP FLPMSRAEMA ALGWDACDVI LVAGDAYIDH PSFGMALVGR
LLEAQGYRVG IISQPDWHSA DAFRVLGQPT LYFGVTAGNM DSMVNRYTSD RKIRSDDAYT
ANAEPNKRPD RAVTVYAQRC REAFPGTPVI IGSIEASLRR IAHYDYWSDT VRRSVLPDSK
ADLLIFGNAE RALLDISHRL AKGENIADIR DVRGTGFMVA RDWKPDEAWI EVLSTELDTP
GAIDAHVDPY AMTPAGPTAQ TPEGTASIKT APVRIVPKAE RLAARQELRA RTVIRLPDYD
QVRNNPALYA HASRVFHLES NPGNARALRQ GHGERDVWLN PPPIPLTTPE MDAVYDLPYA
RAPHPSYGDA KIPAWEMIRF SINIMRGCFG GCTFCSITEH EGRIIQSRSE DSVIREIEAI
RDKTPGFTGV ISDLGGPTAN MYRMACKSKQ IEQSCRRLSC VFPDICENMT TDHTPLIQMY
RRARAVPGIK KIQISSGLRY DLAVRSPEYV KELVQHHVGG YLKIAPEHTE AGPLSKMMKP
GMGAYERFKD MFEAASKAVG KKQYLIPYFI AAHPGTTDED MLNLALWLKK HDFRLDQVQT
FLPTPMALAT AMYHTEKNPL KKVLGGKAET VPVVRQGRTR RLHKAFLRYH DPDNWPLLRQ
ALKDMGREDL IGNGKKHLIP LYQPAGTGRQ PEGARSPCAA RAASGQAHGS KAGAGKPAAN
KAAAVNTSAS KPSAGKPAPA SRGRPPQVPK HPLSQRRGGG KSR
//
