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Database: UniProt
Entry: Q3SZP0
LinkDB: Q3SZP0
Original site: Q3SZP0 
ID   IRF1_BOVIN              Reviewed;         322 AA.
AC   Q3SZP0; Q865L0;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-SEP-2014, entry version 77.
DE   RecName: Full=Interferon regulatory factor 1;
DE            Short=IRF-1;
GN   Name=IRF1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Davis J.S., Hou X.;
RT   "Molecular cloning of Bos taurus interferon regulatory factor 1.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcriptional regulator which displays a remarkable
CC       functional diversity in the regulation of cellular responses.
CC       These include the regulation of IFN and IFN-inducible genes, host
CC       response to viral and bacterial infections, regulation of many
CC       genes expressed during hematopoiesis, inflammation, immune
CC       responses and cell proliferation and differentiation, regulation
CC       of the cell cycle and induction of growth arrest and programmed
CC       cell death following DNA damage. Stimulates both innate and
CC       acquired immune responses through the activation of specific
CC       target genes and can act as a transcriptional activator and
CC       repressor regulating target genes by binding to an interferon-
CC       stimulated response element (ISRE) in their promoters. Its target
CC       genes for transcriptional activation activity include: genes
CC       involved in anti-viral response, such as IFN-alpha/beta,
CC       DDX58/RIG-I, TNFSF10/TRAIL, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and
CC       RSAD2/viperin; antibacterial response, such as NOS2/INOS; anti-
CC       proliferative response, such as p53/TP53, LOX and CDKN1A;
CC       apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune
CC       response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA
CC       damage responses and DNA repair, such as POLQ/POLH; MHC class I
CC       expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and
CC       B2M and MHC class II expression, such as CIITA. Represses genes
CC       involved in anti-proliferative response, such as BIRC5/survivin,
CC       CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as
CC       FOXP3, IL4, ANXA2 and TLR4. Stimulates p53/TP53-dependent
CC       transcription through enhanced recruitment of EP300 leading to
CC       increased acetylation of p53/TP53. Plays an important role in
CC       immune response directly affecting NK maturation and activity,
CC       macrophage production of IL12, Th1 development and maturation of
CC       CD8+ T-cells. Also implicated in the differentiation and
CC       maturation of dendritic cells and in the suppression of regulatory
CC       T (Treg) cells development. Acts as a tumor suppressor and plays a
CC       role not only in antagonism of tumor cell growth but also in
CC       stimulating an immune response against tumor cells (By
CC       similarity).
CC   -!- ENZYME REGULATION: Activated by MYD88 (By similarity).
CC   -!- SUBUNIT: Monomer. Homodimer. Interacts with EP300, PIAS3 and MYD88
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By
CC       similarity). Note=MYD88-associated IRF1 migrates into the nucleus
CC       more efficiently than non-MYD88-associated IRF1 (By similarity).
CC   -!- PTM: Phosphorylated by CK2 and this positively regulates its
CC       activity (By similarity).
CC   -!- PTM: Sumoylation represses the transcriptional activity and
CC       displays enhanced resistance to protein degradation. Inactivates
CC       the tumor suppressor activity. Elevated levels in tumor cells.
CC       Major site is Lys-276. Sumoylation is enhanced by PIAS3 (By
CC       similarity). Desumoylated by SENP1 in tumor cells and appears to
CC       compete with ubiquitination on C-terminal sites (By similarity).
CC   -!- PTM: Ubiquitinated. Appears to compete with sumoylation on C-
CC       terminal sites (By similarity).
CC   -!- SIMILARITY: Belongs to the IRF family.
CC   -!- SIMILARITY: Contains 1 IRF tryptophan pentad repeat DNA-binding
CC       domain.
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DR   EMBL; AY204899; AAO46998.1; -; mRNA.
DR   EMBL; BC102766; AAI02767.1; -; mRNA.
DR   RefSeq; NP_001178190.1; NM_001191261.2.
DR   RefSeq; XP_003584774.1; XM_003584726.2.
DR   UniGene; Bt.102576; -.
DR   UniGene; Bt.87587; -.
DR   UniGene; Bt.89067; -.
DR   ProteinModelPortal; Q3SZP0; -.
DR   SMR; Q3SZP0; 7-111.
DR   STRING; 9913.ENSBTAP00000041732; -.
DR   PRIDE; Q3SZP0; -.
DR   Ensembl; ENSBTAT00000044218; ENSBTAP00000041732; ENSBTAG00000031231.
DR   GeneID; 337917; -.
DR   GeneID; 789216; -.
DR   KEGG; bta:337917; -.
DR   KEGG; bta:789216; -.
DR   CTD; 3659; -.
DR   eggNOG; NOG42582; -.
DR   GeneTree; ENSGT00740000114956; -.
DR   HOGENOM; HOG000037937; -.
DR   HOVERGEN; HBG003455; -.
DR   InParanoid; Q3SZP0; -.
DR   KO; K09444; -.
DR   OrthoDB; EOG72ZCFD; -.
DR   TreeFam; TF328512; -.
DR   Reactome; REACT_215067; Factors involved in megakaryocyte development and platelet production.
DR   Reactome; REACT_218986; TRAF6 mediated IRF7 activation.
DR   NextBio; 20929556; -.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000790; C:nuclear chromatin; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IEA:Ensembl.
DR   GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Ensembl.
DR   GO; GO:0006915; P:apoptotic process; ISS:UniProtKB.
DR   GO; GO:0043374; P:CD8-positive, alpha-beta T cell differentiation; IEA:Ensembl.
DR   GO; GO:0007050; P:cell cycle arrest; ISS:UniProtKB.
DR   GO; GO:0035458; P:cellular response to interferon-beta; IEA:Ensembl.
DR   GO; GO:0071260; P:cellular response to mechanical stimulus; IEA:Ensembl.
DR   GO; GO:0051607; P:defense response to virus; ISS:UniProtKB.
DR   GO; GO:0060333; P:interferon-gamma-mediated signaling pathway; ISS:UniProtKB.
DR   GO; GO:0045590; P:negative regulation of regulatory T cell differentiation; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0032728; P:positive regulation of interferon-beta production; ISS:UniProtKB.
DR   GO; GO:0045084; P:positive regulation of interleukin-12 biosynthetic process; IEA:Ensembl.
DR   GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Ensembl.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0032481; P:positive regulation of type I interferon production; ISS:UniProtKB.
DR   GO; GO:2000564; P:regulation of CD8-positive, alpha-beta T cell proliferation; ISS:UniProtKB.
DR   GO; GO:0034124; P:regulation of MyD88-dependent toll-like receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR017431; Interferon_reg_fac-1/2.
DR   InterPro; IPR019817; Interferon_reg_fac_CS.
DR   InterPro; IPR001346; Interferon_reg_fact_DNA-bd_dom.
DR   InterPro; IPR011991; WHTH_DNA-bd_dom.
DR   Pfam; PF00605; IRF; 1.
DR   PIRSF; PIRSF038196; IFN_RF1/2; 1.
DR   PRINTS; PR00267; INTFRNREGFCT.
DR   SMART; SM00348; IRF; 1.
DR   PROSITE; PS00601; IRF_1; 1.
DR   PROSITE; PS51507; IRF_2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Activator; Antiviral defense; Complete proteome;
KW   Cytoplasm; DNA-binding; Immunity; Innate immunity; Isopeptide bond;
KW   Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription;
KW   Transcription regulation; Tumor suppressor; Ubl conjugation.
FT   CHAIN         1    322       Interferon regulatory factor 1.
FT                                /FTId=PRO_0000223674.
FT   DNA_BIND      5    113       IRF tryptophan pentad repeat.
FT   MOD_RES      78     78       N6-acetyllysine (By similarity).
FT   CROSSLNK    276    276       Glycyl lysine isopeptide (Lys-Gly)
FT                                (interchain with G-Cter in SUMO) (By
FT                                similarity).
FT   CROSSLNK    296    296       Glycyl lysine isopeptide (Lys-Gly)
FT                                (interchain with G-Cter in SUMO) (By
FT                                similarity).
FT   CONFLICT    302    302       L -> W (in Ref. 1; AAO46998).
SQ   SEQUENCE   322 AA;  36168 MW;  273CA27DAE5DB7BB CRC64;
     MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK DACLFRSWAI
     HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPPLTKSQR
     KERKSKSSRD ARSKAKKKPY GEYSPDTFSD GLSSSTLPDD HSNYTVRSYM GQDLDIERTL
     TPALSPCGVS STLPNWSIPV EIVPDSTSDL YNFQVSPMPS TSEAATDEDE EGKLTEDIMK
     LLEQTGWQQT SVDGKGYLLN EPGAQPTSVY GEFSCKEEPE VDSPGGYIGL ISSDMKNMDP
     SLLDSLLTPV RLPSIQAIPC AP
//
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